Macrophage plasticity: signaling pathways, tissue repair, and regeneration DOI Creative Commons
Lingfeng Yan, Jue Wang,

Xin Cai

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: Aug. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Language: Английский

Skin Wound Healing: Normal Macrophage Function and Macrophage Dysfunction in Diabetic Wounds DOI Creative Commons
Savannah Margaret Aitcheson, Francesca D. Frentiu, Sheree E. Hurn

et al.

Molecules, Journal Year: 2021, Volume and Issue: 26(16), P. 4917 - 4917

Published: Aug. 13, 2021

Macrophages play a prominent role in wound healing. In the early stages, they promote inflammation and remove pathogens, debris, cells that have apoptosed. Later repair process, dampen secrete factors regulate proliferation, differentiation, migration of keratinocytes, fibroblasts, endothelial cells, leading to neovascularisation closure. The macrophages coordinate this process are complex: originate from different sources distinct phenotypes with diverse functions act at various times process. individuals diabetes altered, displaying hyperresponsiveness inflammatory stimulants increased secretion pro-inflammatory cytokines. They also reduced ability phagocytose pathogens efferocytose undergone apoptosis. This leads capacity and, as efferocytosis is trigger for their phenotypic switch, it reduces number M2 reparative wound. can lead diabetic foot ulcers (DFUs) forming contributes risk not healing becoming infected, potentially, amputation. Understanding macrophage dysregulation DFUs how these might be along associated inflammation, will ultimately allow better therapies complement current treatment increase DFU's rates.

Language: Английский

Citations

276
Anti‐inflammatory hydrogel dressings and skin wound healing DOI Creative Commons
Can Huang, Lanlan Dong,

Baohua Zhao

et al.

Clinical and Translational Medicine, Journal Year: 2022, Volume and Issue: 12(11)

Published: Nov. 1, 2022

Abstract Hydrogels are promising and widely utilized in the biomedical field. In recent years, anti‐inflammatory function of hydrogel dressings has been significantly improved, addressing many clinical challenges presented ongoing endeavours to promote wound healing. Wound healing is a cascaded highly complex process, especially chronic wounds, such as diabetic severe burn which adverse endogenous or exogenous factors can interfere with inflammatory regulation, leading disruption process. Although insufficient inflammation uncommon, excessive infiltration an almost universal feature impedes histological repair predictable biological step chronological order. Therefore, resolving essential. past 5 extensive research conducted on address healing, specifically by efficiently scavenging free radicals, sequestering chemokines promoting M 1 ‐to‐M 2 polarization macrophages, thereby regulating this study, we introduced novel demonstrated innovative methods for their preparation application achieve enhanced addition, summarize most important properties required discuss our analysis potential yet be addressed.

Language: Английский

Citations

237
Microenvironment‐Based Diabetic Foot Ulcer Nanomedicine DOI Creative Commons

Fang Huang,

Xiangyu Lu, Yan Yang

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(2)

Published: Nov. 24, 2022

Diabetic foot ulcers (DFU), one of the most serious complications diabetes, are essentially chronic, nonhealing wounds caused by diabetic neuropathy, vascular disease, and bacterial infection. Given its pathogenesis, DFU microenvironment is rather complicated characterized hyperglycemia, ischemia, hypoxia, hyperinflammation, persistent However, current clinical therapies for dissatisfactory, which drives researchers to turn attention advanced nanotechnology address therapeutic bottlenecks. In last decade, a large number multifunctional nanosystems based on have been developed with positive effects in therapy, forming novel concept "DFU nanomedicine". systematic overview nanomedicine still unavailable literature. This review summarizes microenvironmental characteristics DFU, presents main progress wound healing, summaries state-of-the-art strategies DFU. Furthermore, challenges future perspectives this field discussed prospected, aiming fuel foster development nanomedicines successfully.

Language: Английский

Citations

200
Macrophage-Mediated Inflammation in Skin Wound Healing DOI Creative Commons

Alireza Hassanshahi,

Mohammad Moradzad, Saman Ghalamkari

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(19), P. 2953 - 2953

Published: Sept. 21, 2022

Macrophages are key immune cells that respond to infections, and modulate pathophysiological conditions such as wound healing. By possessing phagocytic activities through the secretion of cytokines growth factors, macrophages pivotal orchestrators inflammation, fibrosis, repair. orchestrate process healing transitioning from predominantly pro-inflammatory (M1-like phenotypes), which present early post-injury, anti-inflammatory (M2-like appear later skin repair closure. In this review, different cellular molecular aspects macrophage-mediated discussed, alongside important macrophage subtypes, metabolism, plasticity, epigenetics. We also highlight previous studies demonstrating interactions between these factors for optimal Understanding harnessing activity capability may help advance new approaches improving skin.

Language: Английский

Citations

164
Inflammatory Microenvironment of Skin Wounds DOI Creative Commons
Zhen Wang,

Qi Fang,

Han Luo

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 1, 2022

Wound healing is a dynamic and highly regulated process that can be separated into three overlapping interdependent phases: inflammation, proliferation, remodelling. This review focuses on the inflammation stage, as it key stage of wound plays vital role in local immune response determines progression healing. Inflammatory cells, main effector cells inflammatory response, have been widely studied, but little attention has paid to immunomodulatory effects non-inflammatory extracellular matrix. In this review, we attempt deepen our understanding wound-healing microenvironment by focusing interactions between matrix, well their regulating during stage. We hope findings will provide new ideas for promoting tissue regeneration through regulation.

Language: Английский

Citations

106
Novel bi-layered dressing patches constructed with radially-oriented nanofibrous pattern and herbal compound-loaded hydrogel for accelerated diabetic wound healing DOI
Shaohua Wu, Wenwen Zhao,

Mingchao Sun

et al.

Applied Materials Today, Journal Year: 2022, Volume and Issue: 28, P. 101542 - 101542

Published: June 4, 2022

Language: Английский

Citations

94
Intelligent microneedle patch with prolonged local release of hydrogen and magnesium ions for diabetic wound healing DOI Creative Commons

Pei Wang,

Jiayingzi Wu, Haiyan Yang

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 24, P. 463 - 476

Published: Jan. 6, 2023

Diabetes mellitus, an epidemic with a rapidly increasing number of patients, always leads to delayed wound healing associated consistent pro-inflammatory M1 polarization, decreased angiogenesis and increased reactive oxygen species (ROS) in the microenvironment. Herein, poly (lactic-co-glycolic acid) (PLGA)-based microneedle patch loaded magnesium hydride (MgH2) (MN-MgH2) is manufactured for defeating diabetic wounds. The application contributes transdermal delivery prolonged release MgH2 that can generate hydrogen (H2) ions (Mg2+) after reaction body fluids. released H2 reduces production ROS, transforming pathological microenvironment induced by diabetes mellitus. Meanwhile, Mg2+ promotes polarization pro-healing M2 macrophages. Consequently, cell proliferation migration are improved, tissue regeneration enhanced. Such intelligent provides novel way accelerating through steadily preserving releasing locally sustainably.

Language: Английский

Citations

94
Macrophage polarization in diabetic wound healing DOI
Xingqian Wu, Wenjie He, Xingrui Mu

et al.

Burns & Trauma, Journal Year: 2022, Volume and Issue: 10

Published: Jan. 1, 2022

Language: Английский

Citations

91
Exosome/metformin-loaded self-healing conductive hydrogel rescues microvascular dysfunction and promotes chronic diabetic wound healing by inhibiting mitochondrial fission DOI Creative Commons
Yue Zhang, Meng Li, Yunchuan Wang

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 26, P. 323 - 336

Published: March 15, 2023

Chronic diabetic wounds remain a globally recognized clinical challenge. They occur due to high concentrations of reactive oxygen species and vascular function disorders. A promising strategy for wound healing is the delivery exosomes, comprising bioactive dressings. Metformin activates endothelial growth factor pathway, thereby improving angiogenesis in hyperglycemic states. However, multifunctional hydrogels loaded with drugs substances synergistically promote repair has been rarely reported, mechanism their combinatorial effect exosome metformin remains unclear. Here, we engineered dual-loaded possessing tissue adhesive, antioxidant, self-healing electrical conductivity properties, wherein 4-armed SH-PEG cross-links Ag+, which minimizes damage goods investigated promotion repair. Multiwalled carbon nanotubes exhibiting good were also incorporated into generate hydrogen bonds thiol group, creating stable three-dimensional structure loading. The model present study suggests that PEG/Ag/CNT-M + E hydrogel promotes by triggering cell proliferation relieving peritraumatic inflammation injury. involves reducing level interfering mitochondrial fission, protecting F-actin homeostasis alleviating microvascular dysfunction. Hence, propose drug-bioactive substance combination therapy provide potential developing function-associated strategies treating chronic wounds.

Language: Английский

Citations

82
Hypoxic ADSC-derived exosomes enhance wound healing in diabetic mice via delivery of circ-Snhg11 and induction of M2-like macrophage polarization DOI Open Access
Rongfeng Shi,

Yinpeng Jin,

Suming Zhao

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 153, P. 113463 - 113463

Published: Aug. 5, 2022

Diabetes mellitus is a major cause of blindness and chronic ulcers in the working age population worldwide. Former research have found that differentially expressed circular RNAs (circRNAs) are associated with hyperglycemia (HG)-induced endothelial cell damage. And adipose-derived stem cells (ADSCs)-exosome transplant more therapeutic effect to enhance wound healing diabetic mice by delivery circRNA. The current investigation employed high-throughput sequencing identify circRNAs abnormally progenitor (EPCs) under HG conditions. regulatory mechanism predicted targets one circRNA, circ-Snhg11, were investigated utilizing bioinformatics analyses, luciferase reporter assays, angiogenic differentiation flow cytometric apoptosis analysis, RT-qPCR. result show circ-Snhg11 expression decreased EPCs conditions overexpression suppressed HG-induced damage M1-like macrophage polarization. miR-144-3p HIF-1α identified as downstream which was further verified analysis. or inhibition reversed protective on dysfunction, evidenced increased apoptosis, abnormal vascular differentiation, secretion inflammatory factors. In addition, regarding M2-like polarization These findings suggest promotes through sponging. Our data demonstrate exosome from ADSCs suppresses induces via miR-144-3p/HIF-1α axis.

Language: Английский

Citations

81