Dysregulated CD4+ T Cells and microRNAs in Myocarditis

Frontiers in Immunology, Journal Year: 2020, Volume and Issue: 11

Published: March 25, 2020

Myocarditis is a polymorphic disease complicated with indeterminate etiology and pathogenesis, represents one of the most challenging clinical problems lacking specific diagnosis effective therapy. It caused by complex interplay environmental genetic factors, causal links between dysregulated microribonucleic acids (miRNAs) myocarditis have also been supported recent epigenetic researches. Both CD4+ T cells miRNAs play critical roles in pathogenesis myocarditis, classic triphasic model its consists acute infectious, subacute immune, recovery/chronic myopathic phase. are key pathogenic factors underlying development progression effector regulatory subsets respectively promote inhibit autoimmune responses. Furthermore, reciprocal these influences as well. Dysregulated along their mRNA protein targets identified heart biopsies (intracellular miRNAs) body fluids (circulating during myocarditis. These show phase-dependent changes, correlate viral infection, immune status, fibrosis, destruction cardiomyocytes, arrhythmias, cardiac functions outcomes. Thus, promising diagnostic markers therapeutic In this review, we review an emphasis on present summary current knowledge

Language: Английский

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Unveiling ncRNA regulatory axes in atherosclerosis progression

Clinical and Translational Medicine, Journal Year: 2020, Volume and Issue: 9(1)

Published: Jan. 1, 2020

Abstract Completion of the human genome sequencing project highlighted richness cellular RNA world, and opened door to discovery a plethora short long non‐coding RNAs (the dark transcriptome) with regulatory or structural potential, which shifted balance pathological gene alterations from coding RNAs. Thus, disease risk assessment currently has also evaluate expression new such as small micro (miRNAs), (lncRNAs), circular (circRNAs), competing endogenous (ceRNAs), retrogressed elements, 3′UTRs mRNAs, etc. We are interested in pathogenic mechanisms atherosclerosis (ATH) progression patients suffering Chronic Kidney Disease, this review, we will focus role transcriptome (non‐coding RNAs) ATH progression. miRNAs formation axes networks their mRNA targets lncRNAs that function miRNA sponges competitive inhibitors activity. In sense, pay special attention genomic processed pseudogenes Alu repeated have been recently seen sponges, well use derivatives silencing, anti‐ATH therapies. Along discuss technical developments associated research lncRNAs, technologies databases, repositories algorithms predict targets, approaches function, integrative enrichment analysis potential unveil networks.

Language: Английский

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Decoding microRNA drivers in atherosclerosis

Bioscience Reports, Journal Year: 2022, Volume and Issue: 42(7)

Published: June 27, 2022

An estimated 97% of the human genome consists non-protein-coding sequences. As our understanding regulation improves, this has led to characterization a diverse array non-coding RNAs (ncRNA). Among these, micro-RNAs (miRNAs) belong short ncRNA class (22-25 nucleotides in length), with approximately 2500 miRNA genes encoded within genome. From therapeutic perspective, there is interest exploiting as biomarkers disease progression and response treatments, well mimics/repressors novel medicines. have emerged an important RNA master regulators roles identified pathogenesis atherosclerotic cardiovascular disease. Atherosclerosis characterized by chronic inflammatory build-up, driven largely low-density lipoprotein cholesterol accumulation artery wall vascular injury, including endothelial dysfunction, leukocyte recruitment remodelling. Conventional therapy focuses on lifestyle interventions, blood pressure-lowering medications, high-intensity statin antiplatelet agents. However, significant proportion patients remain at increased risk This continued referred residual risk. Hence, new drug targeting atherosclerosis could synergise existing therapies optimise outcomes. Here, we review current role ncRNA, focus miRNA, development atherosclerosis, highlighting biological mechanisms avenues.

Language: Английский

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The MiR-139-5p and CXCR4 axis may play a role in high glucose-induced inflammation by regulating monocyte migration

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 25, 2025

MicroRNAs, a class of small non-coding RNA molecules that regulate gene expression post-transcriptionally, are implicated in various pathological conditions including diabetes mellitus (DM). DM has been increasingly recognized as an inflammatory disease and monocytes play key role propagating inflammation under hyperglycemic conditions. We hypothesize high glucose dysregulates microRNAs to promote monocyte activity, which may contribute the pathogenesis DM. THP-1 were cultured normal (5 mM) (25 RT-qPCR Western blotting performed assay proteins, respectively. Monocytes transfected with microRNA mimics using Lipofectamine RNAiMAX reagent. growth was assessed Calcein-AM dye Boyden chamber applied measure migration. The results showed downregulated miR-139-5p associated increased protein CXCR4, experimentally validated target miR-139-5p. Correspondingly, treatment resulted significant increase cell migration towards SDF-1, cognate ligand for CXCR4. MiR-139-5p overexpression inhibited glucose-induced CXCR4 expression, leading reduced SDF-1. High did not affect growth. In conclusion, miR-139-5p-CXCR4 axis by regulating

Language: Английский

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MicroRNA Expression in Patients with Coronary Artery Disease and Hypertension—A Systematic Review

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6430 - 6430

Published: June 11, 2024

Coronary artery disease (CAD) and hypertension significantly contribute to cardiovascular morbidity mortality. MicroRNAs (miRNAs) have recently emerged as promising biomarkers therapeutic targets for these conditions. This systematic review conducts a thorough analysis of the literature, with specific focus on investigating miRNA expression patterns in patients CAD hypertension. encompasses an unspecified number eligible studies that employed variety patient demographics research methodologies, resulting diverse profiles. highlights complex involvement miRNAs potential advances diagnostic strategies. Future endeavors are imperative validate findings elucidate precise roles progression, offering avenues innovative tools targeted interventions.

Language: Английский

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Connecting the Dots: How MicroRNAs Link Asthma and Atherosclerosis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3570 - 3570

Published: April 10, 2025

Asthma and atherosclerosis are chronic conditions with distinct pathophysiologies, but overlapping inflammatory mechanisms that suggest a potential common regulatory framework. MicroRNAs (miRNAs), small non-coding RNA molecules modulate gene expression post-transcriptionally, could be key players in linking these disorders. This review outlines how miRNAs contribute to the complex interplay between asthma atherosclerosis, focusing on involved pathways, immune cell regulation vascular remodeling. We discuss specific miRNAs, such as miR-155, miR-21 miR-146a, which have been shown cytokine production T differentiation, impacting respiratory cardiovascular health. The found both emphasize their role biomarkers, also therapeutic targets. Understanding molecular connections may unlock novel approaches for innovative, integrated treatment strategies address significantly improve patient outcomes. Further research is needed explore mechanistic pathways validate translational of miRNA-based interventions preclinical clinical settings.

Language: Английский

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Análise funcional dos miRNAs let-7f-5p, miR-10b-5p, miR-34a-5p, miR-181b-5p e miR-181d-5p em células de leiomioma e leiomiossarcoma uterino

Published: March 18, 2021

MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of

Language: Английский

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Synthesis and characterization of polyurethane films based on castor oil-derived polyols with heparin and low-molecular-weight chitosan for cardiovascular implants

Journal of materials research/Pratt's guide to venture capital sources, Journal Year: 2023, Volume and Issue: 38(13), P. 3349 - 3361

Published: June 18, 2023

Abstract Applications of polyurethanes (PUs) depend on several physicochemical factors due to the presence varied monomers involving ratios. The present study aimed synthesize various chitosan/heparin-inserted PUs examine addition effect mechanical and biological properties PU-based polymeric material. were synthesized from unmodified castor oil isophorone diisocyanate, including chitosan heparin as fillers. FTIR, thermal, characterization materials with without fillers exhibited that can be considered within accepted values. In addition, considering non-cytotoxicity antibacterial activity, these polymers exploited biomaterials. results also showed offer a competitive advantage over other since observed good found optimal range for biomaterial design myocardial or vascular tissue engineering. Graphical abstract

Language: Английский

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Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise

Physiological Reports, Journal Year: 2020, Volume and Issue: 8(9)

Published: May 1, 2020

High-intensity interval (HII) exercise elicits distinct vascular responses compared to a matched dose of moderate intensity continuous (MOD) exercise. However, the acute effects HII MOD on arterial stiffness are incompletely understood. Circulating microRNAs (ci-miRs) may contribute We sought determine intensity-dependent changes in ci-miR potentially underlying stiffness. Ten young, healthy men underwent well-matched, 30-min and bouts. RT-qPCR was used levels seven vascular-related ci-miRs serum obtained immediately before after Arterial measures including carotid femoral pulse wave velocity (cf-PWV), compliance β-stiffness, augmentation index (AIx AIx75) were taken before, 10min 60min Ci-miR-21-5p, 126-3p, 126-5p, 150-5p, 155-5p, 181b-5p increased (p < .05), while ci-miR-150-5p 221-3p = .03 0.056). One hour exercise, cf-PWV trended toward being lower baseline .056) significantly .04). Carotid .049) greater than .02). AIx75 10 min both .05). There significant correlations between some exercise-induced individual AIx/AIx75. These results support hypotheses that altered an manner,

Language: Английский

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Identification of Potential miRNA-mRNA Regulatory Network Contributing to Parkinson’s Disease

Parkinson s Disease, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 12

Published: Sept. 5, 2022

Parkinson's disease (PD) is a common neurodegenerative disease, and the mechanism underlying PD pathogenesis not completely understood. Increasing evidence indicates that microRNAs (miRNAs) play critical regulatory role in of PD. This study aimed to explore miRNA-mRNA network for The differentially expressed miRNAs (DEmis) genes (DEGs) between patients healthy donors were screened from miRNA dataset GSE16658 mRNA GSE100054 downloaded Gene Expression Omnibus (GEO) database. Target DEmis selected when they predicted by three or four online databases overlapped with DEGs GSE100054. Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis then conducted Database Annotation, Visualization Integrated Discovery (DAVID) Metascape analytic tools. correlation was evaluated tool Comparative Toxicogenomics (CTD), protein-protein interaction (PPI) networks built STRING platform. We further investigated expression blood samples collected via qRT-PCR. identified 1505 upregulated 1302 downregulated DEGs, 77 112 preliminarily GEO Further functional 10 PD-related hub genes, including

Language: Английский

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