Water,
Journal Year:
2023,
Volume and Issue:
15(3), P. 399 - 399
Published: Jan. 18, 2023
Fish
are
among
the
main
target
groups
when
conducting
biomonitoring
and
ecological
assessment
of
aquatic
environments.
Environmental
DNA
(eDNA)
metabarcoding
provides
a
unique
opportunity
to
enhance
throughput,
precision,
standardization.
However,
few
fish
monitoring
studies
based
on
eDNA
have
been
conducted
at
basin
scale.
Taking
Dianchi
Lake
three
inflowing
rivers
(Panlong
River,
Baoxiang
Chai
River)
as
research
objects,
this
study
monitored
diversity
via
metabarcoding,
Index
Biological
Integrity
(F-IBI)
was
constructed
applied
for
environment.
Here,
we
detected
41
species
belonging
9
orders,
15
families,
35
genera
in
sampling
areas,
including
17
native
such
Hemiculter
leucisculus,
Cyprinus
carpio,
Silurus
soldatovi.
Distinct
patterns
were
observed
rivers.
Particularly,
Panlong
River
richer
than
that
Lake,
River.
Water
temperature
(WT),
chemical
oxygen
demand
(COD),
total
nitrogen
(TN),
phosphorus
(TP)
significantly
influenced
distribution
communities
most
sites
(p
<
0.05).
Moreover,
according
F-IBI
system,
25%
defined
‘fine’
or
above,
75%
‘impaired’
below.
This
provided
insights
into
restoration
precise
management
environment
basin.
Biology,
Journal Year:
2022,
Volume and Issue:
11(9), P. 1277 - 1277
Published: Aug. 27, 2022
The
implementation
of
DNA
metabarcoding
and
environmental
(eDNA)
to
the
biodiversity
assessment
biomonitoring
aquatic
ecosystems
has
great
potential
worldwide.
However,
eDNA
are
highly
reliant
on
coverage
barcode
reference
libraries
that
currently
hindered
by
substantial
lack
sequences.
main
objective
this
study
was
analyze
current
for
phytoplankton
species
Mediterranean
ecoregion
in
southeast
Italy
(Apulia
Region)
order
assess
applicability
area.
To
do
so,
we
investigated
three
libraries,
BOLD
Systems,
GenBank
SILVA,
availability
barcodes
examined
species.
gap
analysis
conducted
molecular
gene
markers,
18S,
16S
COI.
results
showed
a
considerable
all
markers.
among
18S
had
greater
libraries.
For
marker,
across
types
32.21-39.68%,
60.12-65.19%
marker
gene,
72.44-80.61
COI
gene.
Afterwards,
interspecific
genetic
distance
most
represented
able
distinguish
80%
mined
lakes
70%
both
marine
transitional
waters.
Conclusively,
work
highlights
importance
filling
gaps
constitutes
basis
towards
advancement
application
biomonitoring.
Water,
Journal Year:
2023,
Volume and Issue:
15(3), P. 399 - 399
Published: Jan. 18, 2023
Fish
are
among
the
main
target
groups
when
conducting
biomonitoring
and
ecological
assessment
of
aquatic
environments.
Environmental
DNA
(eDNA)
metabarcoding
provides
a
unique
opportunity
to
enhance
throughput,
precision,
standardization.
However,
few
fish
monitoring
studies
based
on
eDNA
have
been
conducted
at
basin
scale.
Taking
Dianchi
Lake
three
inflowing
rivers
(Panlong
River,
Baoxiang
Chai
River)
as
research
objects,
this
study
monitored
diversity
via
metabarcoding,
Index
Biological
Integrity
(F-IBI)
was
constructed
applied
for
environment.
Here,
we
detected
41
species
belonging
9
orders,
15
families,
35
genera
in
sampling
areas,
including
17
native
such
Hemiculter
leucisculus,
Cyprinus
carpio,
Silurus
soldatovi.
Distinct
patterns
were
observed
rivers.
Particularly,
Panlong
River
richer
than
that
Lake,
River.
Water
temperature
(WT),
chemical
oxygen
demand
(COD),
total
nitrogen
(TN),
phosphorus
(TP)
significantly
influenced
distribution
communities
most
sites
(p
<
0.05).
Moreover,
according
F-IBI
system,
25%
defined
‘fine’
or
above,
75%
‘impaired’
below.
This
provided
insights
into
restoration
precise
management
environment
basin.
