Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge DOI Creative Commons
Maarten D. Verhoeven, Per Halkjær Nielsen, Morten Simonsen Dueholm

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(12)

Published: Dec. 5, 2023

Biological wastewater treatment relies on complex microbial communities that assimilate nutrients and break down pollutants in the wastewater. Knowledge about physiology metabolism of bacteria plants (WWTPs) may therefore be used to improve efficacy economy treatment. Our current knowledge is largely based 16S rRNA gene amplicon profiling, fluorescence

Language: Английский

Detoxification of copper and zinc from anaerobic digestate effluent by indigenous bacteria: Mechanisms, pathways and metagenomic analysis DOI

Hongbin Yan,

Zhiqiang Gu, Qi Zhang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133993 - 133993

Published: March 9, 2024

Language: Английский

Citations

36
Microbial extracellular polymeric substances in the environment, technology and medicine DOI
Hans‐Curt Flemming, Eric D. van Hullebusch, Brenda J. Little

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Language: Английский

Citations

27
Comprehensive review on recent trends and perspectives of natural exo-polysaccharides: Pioneering nano-biotechnological tools DOI

Onkar Nath Tiwari,

Md. Nazneen Bobby,

Vanitha Kondi

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130747 - 130747

Published: March 12, 2024

Language: Английский

Citations

11
Coupling extracellular glycan composition with metagenomic data in papermill and brewery anaerobic granular sludges DOI Creative Commons
Anna Doloman, Stefan de Bruin, Mark C.M. van Loosdrecht

et al.

Water Research, Journal Year: 2024, Volume and Issue: 252, P. 121240 - 121240

Published: Feb. 2, 2024

Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding microorganisms biosynthesis pathways responsible glycan production. In this study, we analysed samples from sludges treating papermill brewery wastewater, examining glycans composition using metagenome-assembled genomes (MAGs) to explore potential biochemical associated with their Uronic acids were predominant constituents extracellular polymeric substances (EPS) produced by sludges, comprising up 60 % total polysaccharide content. MAGs affiliated Anaerolineacae, Methanobacteriaceae Methanosaetaceae represented majority microbial community (30-50 reads per MAG). Based on analysis MAGs, it appears that Anaerolinea sp. members Methanobacteria class involved production exopolysaccharides within sludges. These findings shed light functional roles industrial treatment systems.

Language: Английский

Citations

9
Non-filamentous bulking of activated sludge induced by graphene oxide: Insights from extracellular polymeric substances DOI

Muchen Yin,

Yaodong Wu, Dongyue Li

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 399, P. 130574 - 130574

Published: March 11, 2024

Language: Английский

Citations

8
Reinvestigating the Composition of Alginate-Like Exopolymers Extracted from Activated Sludge DOI Creative Commons
Ji Li, Xiaodi Hao,

Rozalia Persiani

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(7), P. 3007 - 3015

Published: June 27, 2024

Over the past decade, a significant amount of research work on extracellular polymeric substances has been done "alginate-like exopolysaccharides" (ALE, also called exopolymers"). The term was used based FAO (Food and Agriculture Organization) biopolymer identification test. Although various chemical analyses have conducted to characterize extracted ALE, it remained unclear whether ALE contains two sugar monomers alginates. Aiming obtain direct answer question: are there alginates in from sludge, activated sludge collected wastewater treatment plants different countries, where previously extracted, characterized, reported literature. polymers were these sludges fractionated according standard protocol. monomer composition each fraction analyzed, with special attention presence mannuronic acid (M) guluronic (G), which compose alginate biopolymers. None found EPS, indicating no resembling sludges. possibility other glycan components, such as lipopolysaccharides investigated confirmed.

Language: Английский

Citations

7
Mechanism Involved in Polyvinyl Chloride Nanoplastics Induced Anaerobic Granular Sludge Disintegration: Microbial Interaction Energy, EPS Molecular Structure, and Metabolism Functions DOI
Peng Zheng, Yan Li,

Youpeng Cheng

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(26), P. 11542 - 11553

Published: June 13, 2024

Nanoplastics (NPs) are emerging pollutants and have been reported to cause the disintegration of anaerobic granular sludge (AnGS). However, mechanism involved in AnGS was not clear. In this study, polyvinyl chloride nanoplastics (PVC-NPs) were chosen as target NPs their long-term impact on structure investigated. Results showed that increasing PVC-NPs concentration resulted inhibition acetoclastic methanogens, syntrophic propionate, butyrate degradation, well disintegration. At presence 50 μg·L

Language: Английский

Citations

5
Mechanisms of microbial co-aggregation in mixed anaerobic cultures DOI Creative Commons
Anna Doloman, Diana Z. Sousa

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: July 4, 2024

Abstract Co-aggregation of anaerobic microorganisms into suspended microbial biofilms (aggregates) serves ecological and biotechnological functions. Tightly packed aggregates metabolically interdependent bacteria archaea play key roles in cycling carbon nitrogen. Additionally, applications, such as wastewater treatment, provide a complete metabolic network to convert complex organic material. Currently, experimental data explaining the mechanisms behind co-aggregation anoxic environments is scarce scattered across literature. To what extent does this process resemble aerobic environments? Does limited availability terminal electron acceptors drive mutualistic relationships, contrary commensal relationships observed oxygen-rich And do co-aggregating archaea, which depend on each other harvest bare minimum Gibbs energy from energy-poor substrates, use similar cellular those used by pathogenic that form biofilms? Here, we an overview current understanding why how mixed communities co-aggregate discuss potential future scientific advancements could improve study aggregates. Key points • Metabolic dependency promotes aggregation Flagella, pili, adhesins role formation Cyclic di-GMP/AMP signaling may trigger polysaccharides production anaerobes

Language: Английский

Citations

5
Metagenomic analysis unveils the underexplored roles of prokaryotic viruses in a full-scale landfill leachate treatment plant DOI
Tianyi Chen,

Chunfang Deng,

Zongzhi Wu

et al.

Water Research, Journal Year: 2023, Volume and Issue: 245, P. 120611 - 120611

Published: Sept. 10, 2023

Language: Английский

Citations

11
Revisiting microbial exopolysaccharides: a biocompatible and sustainable polymeric material for multifaceted biomedical applications DOI

Neelakanta Sarvashiva Kiran,

Chandrashekar Yashaswini,

Sudarshan Singh

et al.

3 Biotech, Journal Year: 2024, Volume and Issue: 14(4)

Published: March 4, 2024

Language: Английский

Citations

4