Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Clinical Microbiology Reviews, Journal Year: 2023, Volume and Issue: 37(1)

Published: Dec. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Language: Английский

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Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods

Communications Medicine, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 7, 2025

Understanding factors associated with antimicrobial resistance (AMR) distribution across populations is a necessary step in planning mitigation measures. While associations between AMR and socioeconomic-status (SES), including employment education have been increasingly recognized low- middle-income settings, connections are less clear high-income countries where SES remains an important influence on other health outcomes. We explored the relationship Calgary, Canada using spatially-resolved wastewater-based surveillance of resistomes detected by metagenomics eight socio-economically diverse urban neighborhoods. Resistomes were established shotgun-sequencing wastewater pellets, qPCR targeted-AMR genes. status was 2021 Canadian census data. Conducting this comparison during height COVID-related international travel restrictions (Dec. 2020–Oct. 2021) allowed hypotheses linking to be assessed limited confounding. These compared sewage metagenomes from 244 cities around world, linked Human Development Index (HDI). Wastewater Calgary's socioeconomically neighborhoods exhibit highly similar resistomes, no quantitative differences (p > 0.05), low Bray-Curtis dissimilarity, significant correlations SES. By comparison, dissimilarity observed globally-sourced < underscoring homogeneity sub-populations. The analysis alongside resistome further reveals lower burden Calgary relative world. This particularly pronounced for most clinically-relevant genes (e.g., beta-lactamases, macrolide-lincosamide-streptogramin). work showcases effectiveness inclusive comprehensive exploring interplay AMR. Antimicrobial occurs when treatments fail microbes continue grow. result acquiring increasing public threat. Some studies suggested association socioeconomic factors. amount can monitored investigating presence specific indicative wastewater. To explore within country publicly funded care, we collected large city. Conducted over eleven months COVID-19-related restrictions, did not observe residents or types also globe, significantly differed along socio-economic parameters. Overall, our findings revealed dynamic, context dependent. Lee et al. investigate whether city associates (SES) resident granular, neighborhood-based approach. No correlation seen, contrast data disproportionately concentrate

Language: Английский

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Subsewershed Analyses of the Impacts of Inflow and Infiltration on Viral Pathogens and Antibiotic Resistance Markers Across a Rural Sewer System

Water Research, Journal Year: 2025, Volume and Issue: 276, P. 123230 - 123230

Published: Jan. 30, 2025

Language: Английский

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Evaluating various composite sampling modes for detecting pathogenic SARS-CoV-2 virus in raw sewage

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 23, 2023

Inadequate sampling approaches to wastewater analyses can introduce biases, leading inaccurate results such as false negatives and significant over- or underestimation of average daily viral concentrations, due the sporadic nature input. To address this challenge, we conducted a field trial within University Tennessee residence halls, employing different composite modes that encompassed time intervals (1 h, 2 4 6 24 h) across various windows (morning, afternoon, evening, late-night). Our primary objective was identify optimal approach for generating representative samples SARS-CoV-2 from raw wastewater. Utilizing reverse transcription-quantitative polymerase chain reaction, quantified levels RNA pepper mild mottle virus (PMMoV) in sewage. findings consistently demonstrated PMMoV RNA, an indicator human fecal contamination water environment, exhibited higher abundance lower variability compared pathogenic RNA. Significantly, both greater 1 h individual throughout entire period, contrasting with stability observed other time-based samples. Through comprehensive analysis using Quade Nonparametric ANCOVA test date, concentration site covariates, concluded sampler during focused morning window is pragmatic cost-effective strategy achieving single day wastewater-based epidemiology applications. This method has potential significantly enhance accuracy reliability data collected at community level, thereby contributing more informed public health decision-making pandemic.

Language: Английский

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Implementing an adaptive, two-tiered SARS-CoV-2 wastewater surveillance program on a university campus using passive sampling

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 168998 - 168998

Published: Nov. 29, 2023

Language: Английский

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Early warning COVID-19 outbreak in long-term care facilities using wastewater surveillance: correlation, prediction, and interaction with clinical and serological statuses

The Lancet Microbe, Journal Year: 2024, Volume and Issue: 5(10), P. 100894 - 100894

Published: Aug. 23, 2024

The unprecedented COVID-19 pandemic has highlighted the strategic value of wastewater-based surveillance (WBS) SARS-CoV-2. This multisite 28-month-long study focused on WBS for older residents in 12 long-term care facilities (LTCFs) Edmonton (AB, Canada) by assessing relationships between COVID-19, WBS, and serostatus during pandemic.

Language: Английский

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The Influence of Sports Habits on Stress Levels of Medical Faculty Students in Surakarta City

Journal La Medihealtico, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 8

Published: Jan. 15, 2024

Stress is a problem in everyday life that familiar to everyone, especially medical students. Exercise can reduce stress and improve mental health. If exercise carried out routinely regularly, it the activity of sympathetic nerves hypothalamic pituitary adrenals. This study aims determine effect habits on levels students at Faculty Medicine Surakarta City. The research used an analytical observational design with cross sectional approach, sample size 136 respondents consisting 4 groups/generations. uses observation, interviews questionnaires. Obtaining data from direct several who were samples this was identify IPAQ (International Physical Activity Questionnaire) which classified into 3, namely light, moderate, high level experienced by using KPDS (Kessler questionnaire Psychological Distress Scale). results tested Spearman rank correlation coefficient test. show there influence Medical students, value sig. (1-tailed) 0.009, smaller than critical limit α = 0.01 (0.009 < 0.01), meaning significant relationship or between levels.

Language: Английский

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Amplitude multiplexed wastewater surveillance for campus health: tracking SARS-CoV-2, influenza A, and norovirus

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: March 15, 2024

This study implements wastewater surveillance on a university campus to monitor highly infectious communal diseases utilizing ddPCR and overcoming the limitation of two fluorescent channels reader by employing an amplitude multiplex.

Language: Английский

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Early Warning COVID-19 Outbreak in Long-Term Care Facilities Using Wastewater Surveillance: Correlation, Prediction, and Interaction with Clinical and Serological Statuses

Published: Jan. 1, 2024

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Language: Английский

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Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighbourhoods

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Understanding factors associated with antimicrobial resistance (AMR) distribution across populations is a necessary step in planning optimal mitigation measures. While associations between AMR and socioeconomic-status (SES), including like income, employment, education, have been increasingly recognized low- middle-income settings, this interplay less clear high-income countries. We explored the relationship SES Calgary, Canada using spatially-resolved wastewater-based surveillance of resistomes detected by metagenomics socio-economically diverse urban neighbourhoods. Conducting comparison during height COVID-related international travel restrictions (Dec.2020-Oct.2021) allowed hypotheses linking to be assessed limited confounding. Wastewater metagenomes from eight neighbourhoods exhibited highly similar resistomes, no quantitative differences (p > 0.05), low Bray-Curtis dissimilarity, significant correlations SES. By comparison, dissimilarity was observed globally-sourced 244 cities < underscoring homogeneity Calgary’s sub-populations. The analysis alongside Calgary resistome further revealed lower burden relative other around world particularly pronounced for some most clinically-relevant genes (e.g., beta-lactamases, macrolide-lincosamide-streptogramin). This work showcases effectiveness inclusive comprehensive exploring AMR.

Language: Английский

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