Bacterial hosts and horizontal transfer characteristics of clinically important tet(X)-variant genes in municipal wastewater treatment plants using epicPCR-directed cultivation strategy DOI

Shiting Dai,

Ziming Han, Shihai Liu

et al.

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122658 - 122658

Published: Oct. 18, 2024

Language: Английский

Wastewater-based epidemiology framework: Collaborative modeling for sustainable disease surveillance DOI Creative Commons
Néstor DelaPaz-Ruíz, Ellen-Wien Augustijn, Mahdi Farnaghi

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 968, P. 178889 - 178889

Published: Feb. 19, 2025

Language: Английский

Citations

1
Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method DOI Creative Commons
Marlee Shaffer, Devin North, Kyle Bibby

et al.

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 4, 2025

Abstract Wastewater-based surveillance has emerged as a powerful approach to monitoring infectious diseases within community. Typically, wastewater samples are concentrated before viral analyses improve sensitivity. Current concentration methods vary in time requirements, costs, and efficiency. Here, we evaluated the efficiency bias of novel approach, Nanotrap Microbiome Particles (NMP), wastewater. NMP was target-specific, with significantly lower concentrations bacterial indicator HF183 Carjivirus (formerly crAssphage) relative direct extraction (1.2 × 10 5 vs. 3.4 GC/mL 2.0 1.2 GC/mL, respectively), but higher fecal Pepper Mild Mottle Virus (PMMoV) (1.4 8.4 3 GC/mL). Targeted metagenomic sequencing showed that resulted more unique species reads per sample than extractions ( p < 0.001) by detecting went undetected extractions. Key families identified high abundances were Adenoviridae, Caliciviridae, Herpesviridae, Papillomaviridae, Polyomaviridae. differential ability for concentrating clinically relevant families, suggesting technology should be optimized specific targets implementation.

Language: Английский

Citations

0
Revisiting the Potential Role of Urine in Wastewater Surveillance for COVID-19 and Beyond DOI Creative Commons
Aaron Bivins

Environmental Science & Technology Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

0
Temporal, Spatial, and Methodological Considerations in Wastewater-Based Epidemiology for Sexually Transmitted Infections DOI
William Chen, Kyle Bibby

ACS ES&T Water, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Language: Английский

Citations

0
Making waves: The benefits and challenges of responsibly implementing wastewater-based surveillance for rural communities DOI
Alasdair Cohen, Peter J. Vikesland, Amy Pruden

et al.

Water Research, Journal Year: 2023, Volume and Issue: 250, P. 121095 - 121095

Published: Dec. 30, 2023

Language: Английский

Citations

6
Estimating rates of change to interpret quantitative wastewater surveillance of disease trends DOI
David Holcomb, Ariel Christensen, Kelly Hoffman

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175687 - 175687

Published: Aug. 22, 2024

Language: Английский

Citations

1
Development of a triplex RT-qPCR assay for simultaneous quantification of Japanese encephalitis, Murray Valley encephalitis, and West Nile viruses for environmental surveillance DOI Creative Commons
Yawen Liu, Wendy Smith,

Metasebia Gebrewold

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(10)

Published: Aug. 20, 2024

ABSTRACT The co-circulation of mosquito-borne Japanese encephalitis virus (JEV), Murray Valley (MVEV), and West Nile (WNV) has impacted human animal health in multiple countries worldwide. To facilitate early warnings surveillance the presence these viral infectious agents environment, a triplex reverse transcription-quantitative PCR (RT-qPCR) was developed for simultaneous quantification JEV, MVEV, WNV potential hotspots such as piggery urban wastewater environmental water samples. performance RT-qPCR assay compared with that simplex counterparts, all using same primer probe sequences. quantifiable results showed concordance rate 93.9%–100% (Cohen’s kappa) between assays. mean concentrations exogenous assays were remarkably similar piggery/urban However, impacts matrix effects (i.e., sample composition inhibition) samples on accurate viruses need to be considered. Taken together, this newly will allow more rapid cost-efficient analysis data interpretation. application may valuable tool complement existing disease mosquito approaches used safeguard both humans animals. IMPORTANCE poses significant threats globally. In study, Results demonstrated high sensitivity assays, indicating its efficacy surveillance. This cost-effective offers vital timely monitoring samples, enhancing our ability mitigate outbreaks public health.

Language: Английский

Citations

0
Temporal, spatial, and methodological considerations in evaluating the viability of measles wastewater surveillance DOI
William Chen, Kyle Bibby

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 959, P. 178141 - 178141

Published: Dec. 21, 2024

Language: Английский

Citations

0
Estimating Rates of Change to Interpret Quantitative Wastewater Surveillance of Disease Trends DOI Creative Commons
David Holcomb, Ariel Christensen, Kelly Hoffman

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 25, 2024

Abstract Background Wastewater monitoring data can be used to estimate disease trends inform public health responses. One commonly estimated metric is the rate of change in pathogen quantity, which typically correlates with clinical surveillance retrospective analyses. However, accuracy estimation approaches has not previously been evaluated. Objectives We assessed performance for estimating rates wastewater loads by generating synthetic time series were known. Each approach was also evaluated on real-world data. Methods Smooth and their first derivatives jointly sampled from Gaussian processes (GP) independent errors added generate viral load measurements; range hyperparameter error variance varied produce nine simulation scenarios representing different potential patterns. The directions magnitudes estimates four (two established two developed this work) compared GP derivative evaluate classification quantitative accuracy. implemented SARS-CoV-2 collected January 2021 – May 2023 at 25 sites North Carolina, USA. Results All inconsistently identified correct direction trend given sign derivative. Across all simulated patterns, between a quarter half indicated wrong direction, regardless approach. proportion classified as plateaus (statistically indistinguishable zero) Carolina considerably method but site. Discussion Our results suggest that measurements alone might provide sufficient reliably track real-time. Instead, could combined additional improve predictions other outcomes.

Language: Английский

Citations

0
Bacterial hosts and horizontal transfer characteristics of clinically important tet(X)-variant genes in municipal wastewater treatment plants using epicPCR-directed cultivation strategy DOI

Shiting Dai,

Ziming Han, Shihai Liu

et al.

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122658 - 122658

Published: Oct. 18, 2024

Language: Английский

Citations

0