Microbes and Environments,
Journal Year:
2024,
Volume and Issue:
39(4), P. n/a - n/a
Published: Jan. 1, 2024
Myxobacteria,
belonging
to
the
phylum
Myxococcota,
are
ubiquitous
in
soil,
marine,
and
other
environments.
A
recent
metagenomic
sequencing
ana-lysis
showed
that
Myxococcota
predominant
activated
sludge
systems;
however,
their
metabolic
traits
remain
unclear.
In
present
study,
we
exami-ned
potential
biological
functions
of
46
bins
reconstructed
from
samples
four
municipal
sewage
treatment
plants.
The
results
obtained
most
had
an
almost
complete
set
genes
associated
with
glycolysis
TCA
cycle.
Palsa-1104
Polyangiales
contained
glycoside
hydrolase
GH5
peptidase
M23,
which
presumably
involved
lysis
cell
wall
cellular
cytoplasm,
suggesting
some
prey
on
microorganisms.
contact-dependent
predatory
Myxococcus
xanthus
conserved
family
Myxococcaceae,
but
not
families.
Two
phototrophic
gene
clusters,
indicating
for
heterotrophic
autotrophic
metabolism
by
these
microbes.
assessments
social
behavior
sludge,
FruA
C-signal
gene,
regulation
fruiting
body
formation,
were
lacking
bins,
inability
form
bodies.
addition,
multiple
novel
secondary
metabolite
biosynthesis
clusters
may
be
used
predation
bacteria
sludge.
Our
metagenome-based
ana-lyses
provide
insights
into
microbial
interactions
ecosystems.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 5, 2025
The
obligate
necrotrophic
parasite,
Candidatus
Mycosynbacter
amalyticus,
a
member
of
the
Patescibacteria
has
been
isolated
from
wastewater.
Subsequent
efforts
have
directed
toward
unravelling
its
biological
lifecycle
and
attachment
mechanism
facilitating
infection
subsequent
lysis
Actinobacterial
host,
Gordonia
amarae.
Here,
using
electron
cryo-tomography
(CryoET),
we
reveal
molecular
anatomy
parasitic
amalyticus
cells,
uncovering
an
unusual
process.
Through
laboratory-based
evolution
experiments,
generated
eleven
slow-growing
independent
spontaneous
amarae
resistant
mutants.
Mycolic
acids
(MA)
are
key
components
outer
cellular
envelope
G.
other
Actinobacteria,
with
MA
being
physical
attribute
implicated
in
associated
wastewater
foaming.
CryoET
genome
sequencing
exposed
absence
intact
suite
mutations
predominantly
occurring
within
pks13
pptT
genes
biosynthetic
pathway.
Our
findings
suggest
that
structural
integrity
is
critical
for
Ca.
to
host.
Nitrous
oxide-reducing
bacteria
(N2ORB)
are
generally
considered
the
only
biological
sink
for
potent
greenhouse
gas
N2O.
Although
N2O
consumption
activities
by
diverse
heterotrophic
N2ORB
have
been
detected,
knowledge
gaps
remain
about
phylogenies,
physiologies,
and
of
N2ORB.
Here,
we
successfully
enriched
a
methylotrophic
consortium
under
intermittent
oxygen
supplies.
15N
tracer
analysis
showed
that
activity
was
higher
than
its
production
in
presence
either
single
or
multiple
electron
acceptors
(i.e.,
nitrogen
oxides).
The
observed
maximum
80.7
μmol·g-biomass-1·h-1.
Quantitative
PCR
results
clade
I
nosZ
overwhelmed
II
at
high
(0.41
mmol·min-1)
low
(0.08
loading
rates.
dilution
rate
affected
microbial
community
composition
activity.
A
stimulated
active
oxygen-tolerant
boosted
approximately
50%
oxygen.
Metagenomic
unraveled
predominance
novel
N2ORB,
possessing
entire
denitrifying
genes
high-affinity
terminal
oxidase
genes,
from
reactor
with
rate.
unique
physiological
traits
methanol
shed
light
on
function─aerobic
N2ORB─and
pave
way
innovative
mitigation
strategies
applying
powerful
sinks
engineered
systems.
Microbiology Resource Announcements,
Journal Year:
2024,
Volume and Issue:
13(2)
Published: Jan. 11, 2024
Myxobacteria
are
known
as
prolific
producers
of
secondary
metabolites
with
a
unique
and
wide
spectrum
bioactivities.
Here,
we
report
draft
genome
sequences
KH5-1
NO1,
myxobacteria
isolated
from
activated
sludge,
which
consist
9.89
9.86
Mb,
both
have
G
+
C
contents
70.7%.
