Transcriptional regulation and overexpression of GST cluster enhances pesticide resistance in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Oct. 19, 2023

The rapid evolution of resistance in agricultural pest poses a serious threat to global food security. However, the mechanisms through metabolic regulation are largely unknown. Here, we found that GST gene cluster was strongly selected North China (NTC) population, and it significantly genetically-linked lambda-cyhalothrin resistance. Knockout using CRISPR/Cas9 increased sensitivity knockout strain lambda-cyhalothrin. Haplotype analysis revealed no non-synonymous mutations or structural variations cluster, whereas GST_119 GST_121 were overexpressed NTC population. Silencing co-silencing with RNAi larval We also identified additional GATAe transcription factor binding sites promoter NTC_GST_119. Transient expression Hi5 cells activated NTC_GST_119 Xinjiang (XJ)_GST_119 transcription, but transcriptional activity higher than XJ_GST_119. These results demonstrate regulatory region result complex changes which enhances field-populations. This study deepens our knowledge evolutionary mechanism adaptation under environmental stress provides potential targets for monitoring integrated management.

Language: Английский

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A midgut transcriptional regulatory loop favors an insect host to withstand a bacterial pathogen

The Innovation, Journal Year: 2024, Volume and Issue: 5(5), P. 100675 - 100675

Published: July 14, 2024

•Increased titer of the insect hormone 20-hydroxyecdysone (20E) facilitates an host, Plutella xylostella, to defeat its bacterial pathogen Bacillus thuringiensis (Bt).•Glucose dehydrogenase (GLD) was identified as a new ecdysone-degrading enzyme that can metabolize 20E.•A midgut miRNA initiated epigenetic regulatory pathway represses GLD activity and elevates 20E resist Bt pathogen.•An as-yet uncharacterized negative feedback loop reduces excess balance hormonal homeostasis.•This study provides insights into immunological landscape classical hormones molecular basis host-pathogen coevolution. Mounting evidence suggests associated with growth development also participate in defense. We have discovered previously undescribed transcriptional control modulates availability worldwide pest (Plutella xylostella), allowing it major virulence factor (Bt). A reduction inhibitor (PxDfd) increases expression microRNA (miR-8545), which turn newly ecdysteroid-degrading glucose (PxGLD). Downregulation PxGLD degradation increase concurrently triggers mitigate overproduction. The moderately elevated activates MAPK signaling tolerance/resistance. These findings deepen our understanding functions attributed these help inform potential future strategies be employed pests.

Language: Английский

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The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner)

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0318154 - e0318154

Published: Jan. 29, 2025

Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera globally distributed crop pest affecting over 300 species. H. has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding genetic basis provides insights to develop tools, such as molecular markers, that can be used slow or prevent evolution resistance. We explore architecture widely insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) wild-caught samples quantitative trait locus (QTL) mapping controlled cross susceptible resistant laboratory strains. Both approaches identified on chromosome 2, revealing SNPs within 976 bp monitor field flubendiamide. This was only region linkage mapping, though GWAS revealed additional sites associated with Other loci by populations contained known detoxification genes from ATP-binding cassette family, ABCA1, ABCA3, ABCF2 MDR1. Our findings an oligogenic architecture, contrasting previous reports monogenic ryanodine receptor . work elucidates evolving will contribute developing effective management strategies.

Language: Английский

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Rapid Adaptation and Interspecific Introgression in the North American Crop Pest Helicoverpa zea

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(7)

Published: June 27, 2024

Insect crop pests threaten global food security. This threat is amplified through the spread of nonnative species and adaptation native to control measures. Adaptations such as pesticide resistance can result from selection on variation within a population, or gene flow another population. We investigate these processes in an economically important noctuid pest, Helicoverpa zea, which has evolved wide range pesticides. Its sister armigera, first detected invasive Brazil 2013, introduced pyrethroid-resistance CYP337B3 South American H. zea via adaptive introgression. To understand whether this could contribute North America, we sequenced 237 genomes across 10 sample sites. report armigera introgression into Two individuals sampled Texas 2019 carry haplotypes 4 Mbp region containing CYP337B3. Next, identify signatures panmictic population nonadmixed identifying selective sweep at second cytochrome P450 gene: CYP333B3. estimate that its derived allele conferred ∼5% fitness advantage show explains independently observed rare nonsynonymous CYP333B3 mutations approaching fixation over ∼20-year period. also detect putative kinesin associated with Bt resistance. Overall, document two mechanisms rapid adaptation: introduction fitness-enhancing alleles interspecific introgression, intraspecific variation.

Language: Английский

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Molecular Mechanisms Underlying Resistance to Bacillus thuringiensis Cry Toxins in Lepidopteran Pests: An Updated Research Perspective

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 155 - 155

Published: Jan. 10, 2025

Genetically modified crops that produce insecticidal proteins from Bacillus thuringiensis (Bt) are currently the most efficient and safest method of pest control worldwide. However, prolonged planting period has led to a reduction in efficacy Bt due evolution resistance field. This review paper examines status lepidopteran pests under field conditions, elucidates molecular mechanism underlying their Cry toxins, discusses management strategies based on these mechanisms. Extensive research demonstrated mutations alterations expression patterns midgut receptor genes closely associated with resistance. As our understanding mechanisms progresses, several innovative approaches such as DNA detection techniques, engineering combining toxin RNAi technology have been developed for effective measures. Future will further unravel intricate this phenomenon develop scientifically sound integrated strategies.

Language: Английский

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Morphogenetic Identification of a New Record Condica capensis (Lepidoptera: Noctuidae) in Yunnan, China

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 130 - 130

Published: Jan. 29, 2025

Condica capensis (Lepidoptera: Noctuidae), a newly identified pest in Yunnan Province, China, poses threat to safflower crops. Discovered Nanhua County November 2023, the damages at multiple life stages, especially during its larval stage, when it feeds on leaves, tender stems, and flower filaments, sometimes causing entire plant die. Morphological molecular analyses, including mitochondrial cytochrome C oxidase I (COI) gene sequencing, confirmed identity as C. capensis, new species record for Yunnan. The study also documented pest’s cycle, reproductive behavior, natural enemies, highlighting potential biological control using parasitic wasps such Cotesia sp. This research emphasizes need accurate identification monitoring develop effective, sustainable management strategies. As cultivation grows Yunnan, managing is critical safeguarding local agriculture preventing broader agricultural threats.

Language: Английский

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Genomic predictions of invasiveness and adaptability of the cotton bollworm in response to climate change

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Dynamic Analysis of an Instantaneous/Non‐Instantaneous Unilateral Diffusion Prey–Predator Model With Impulsive Control Strategy

Mathematical Methods in the Applied Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

ABSTRACT In this paper, we propose and study an instantaneous/non‐instantaneous unilateral diffusion predator–prey model with impulsive chemical biological control strategies. Using the theory comparison theorem of differential equations, obtain sufficient conditions for local asymptotic stability global prey‐free periodic solution. Then, prove permanence system a condition system. Finally, some numerical simulations are provided to verify accuracy our theoretical results further explore complex dynamical behaviors These provide basis coordinating relationship between control.

Language: Английский

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Population genomics reveals population structure and local adaptation of the Helicoverpa armigera lineage in Xinjiang, China

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract BACKGROUND The cotton bollworm, Helicoverpa armigera , is a global pest of many crops, particularly cotton. Populations in Xinjiang (north‐western China) are distinct from other major lineages eastern China and elsewhere. Cotton grown wide range climatic conditions but little known about H. population structure gene exchange, either within or between it the populations. RESULTS Population analyses showed significant flow throughout western‐most collections, Kashi Shawan, were most divergent, both each collections. Demographic history dynamics revealed peak ancestral size for populations during relatively warm humid period 5000–10 000 years ago. Selective sweep found local adaptation genes including several CYP9A two trehalose transport 1 ( Tret1 ) genes, Shawan CYP6AE plus basic juvenile hormone‐suppressible protein BJSP‐1 Methuselah genes. Spikes introgression cyc tim per related to circadian rhythm also detected and/or CONCLUSION genetically differentiated at whole‐genome level. Despite some evidence introgression, they show detoxification, various stress tolerances development. Management strategies against should consider these regional differences. © 2025 Society Chemical Industry.

Language: Английский

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Accurate identification of Helicoverpa armigera–Helicoverpa zea hybrids using genome admixture analysis: implications for genomic surveillance

Frontiers in Insect Science, Journal Year: 2024, Volume and Issue: 4

Published: Feb. 23, 2024

Helicoverpa armigera , the cotton bollworm moth, is one of world’s most important crop pests, and spreading throughout New World from its original range in Old World. In Brazil, invasive H. has been reported to hybridize with local populations zea . The correct identification armigera-H. hybrids understanding origin, spread future outlook for regions that are affected by outbreaks, given hybridization can potentially facilitate pesticide resistance host plant via introgression genes. Here, we present a genome admixture analysis high quality sequences generated two F1 different labs. Our pipeline predicts 48.8% 48.9% hybrids, confirming accuracy. Genome five were as part study show no evidence hybridization. Interestingly, four genomes previous predicted possess proportion genetic material. Using unsupervised clustering identify non-hybridized genomes, 8511 ancestry informative markers (AIMs) identified. Their relative frequencies consistent minor component however origin remains be established. We size genomic reference datasets critical accurate prediction. Consequently, discuss potential pitfalls suggest measures will improve such analyses.

Language: Английский

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