Drug repositioning in the AI-driven era: data, approaches, and challenges DOI
Jing Wang,

Siming Kong,

Xiaochen Bo

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

The advent of artificial intelligence (AI) has revolutionized drug repositioning, transforming it into an indispensable strategy for accelerating discovery. This chapter offers in-depth exploration the multifaceted landscape repositioning in AI era, emphasizing profound influence on this domain and providing a roadmap future research. Beginning with brief summary data that form bedrock field, biomedical databases encompassing drugs, diseases, molecular targets, clinical are introduced detail. Then experimental computational approaches underpin further dissected, ranging from binding assays or phenotypic screening to multi-omics methodologies silico technologies, emphasis AI-driven methods. Subsequently, successful cases across diverse diseases highlighted. Finally, importance fully leveraging address challenges is underscored.

Language: Английский

Organoid models: applications and research advances in colorectal cancer DOI Creative Commons

Yijie Wu,

Sha Yu,

Xingpo Guo

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 7, 2025

This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, high incidence mortality rates are introduced, emphasizing importance organoids as a model. Second, this provides detailed introduction to concept, biological properties, organoids, including their strengths mimicking structural functional aspects organs. article further analyzes adult stem cell-derived pluripotent discusses advancements for basic research, drug development, personalized treatment evaluation prediction, regenerative medicine. Finally, prospects applying technology its significant value improving patient survival rates. In conclusion, systematically explains highlighting tremendous potential promising

Language: Английский

Citations

0
From organoids to organoids-on-a-chip: Current applications and challenges in biomedical research DOI Creative Commons
Kailun Liu,

Xiaowei Chen,

Zhen Fan

et al.

Chinese Medical Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract The high failure rates in clinical drug development based on animal models highlight the urgent need for more representative human biomedical research. In response to this demand, organoids and organ chips were integrated greater physiological relevance dynamic, controlled experimental conditions. This innovative platform—the organoids-on-a-chip technology—shows great promise disease modeling, discovery, personalized medicine, attracting interest from researchers, clinicians, regulatory authorities, industry stakeholders. review traces evolution organoids-on-a-chip, driven by necessity advanced biological models. We summarize applications of simulating pathological phenotypes therapeutic evaluation technology. section highlights how integrating technologies chips, such as microfluidic systems, mechanical stimulation, sensor integration, optimizes organoid cell types, spatial structure, functions, thereby expanding their applications. conclude addressing current challenges offering insights into prospects. advancement is poised enhance fidelity, standardization, scalability. Furthermore, integration cutting-edge interdisciplinary collaborations will be crucial progression

Language: Английский

Citations

0
Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review DOI Creative Commons
Lai Wei,

Hu Geliang,

Bin Xu

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: March 3, 2025

Abstract As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of relies mainly on a type unknown composition scaffold, namely Matrigel, which may pose problems studying effect mechanical properties organoids. Hydrogels, material with adjustable properties, can adapt to current studies. In this review, we summarized synthesis recent advance developing definite hydrogel scaffolds organoid identified critical parameters regulating properties. addition, classified by different like stiffness viscoelasticity, concluded development tumor We hope review enhances understanding hydrogels provides more practical approaches investigating them.

Language: Английский

Citations

0
Multimodal Profiling of Iron Heterogeneity at the Nanoscale DOI
Junzhe Zhang, Wanqin Dai, Weiyou Yang

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Life is inherently heterogeneous, and visualizing this heterogeneity in the spatial distribution of biometals offers valuable insights into various biological processes. While biometal mapping provides superior resolution compared to other bioanalytical techniques, it alone cannot fully explain functional roles health disease. In study, we introduced a novel method using specially designed sample grids facilitate beam-, X-ray-, ion-beam-based imaging on same tissue section. This innovative approach aligns integrates nanoscale-resolved iron profiles across spatial, chemical, isotopic dimensions. By combining these analyses, achieved unprecedented detail, revealing complex regulatory framework homeostasis liver tissues following overload. These findings demonstrate that enhancing both information content analysis can overcome current limitations, providing new molecular mechanisms underlying functions.

Language: Английский

Citations

0
Transforming cancer treatment: integrating patient-derived organoids and CRISPR screening for precision medicine DOI Creative Commons

Ziyi Zhu,

John Paul Shen,

Paul Chi-Lui Ho

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 25, 2025

The persistently high mortality rates associated with cancer underscore the imperative need for innovative, efficacious, and safer therapeutic agents, as well a more nuanced understanding of tumor biology. Patient-derived organoids (PDOs) have emerged innovative preclinical models significant translational potential, capable accurately recapitulating structural, functional, heterogeneous characteristics primary tumors. When integrated cutting-edge genomic tools such CRISPR, PDOs provide powerful platform identifying driver genes novel targets. This comprehensive review delves into recent advancements in CRISPR-mediated functional screens leveraging across diverse types, highlighting their pivotal role high-throughput genomics microenvironment (TME) modeling. Furthermore, this highlights synergistic potential integrating CRISPR immunotherapy, focusing on uncovering immune evasion mechanisms improving efficacy immunotherapeutic approaches. Together, these technologies offer promise advancing precision oncology.

Language: Английский

Citations

0
Multi-omics in immunotherapy research for HNSCC: present situation and future perspectives DOI Creative Commons
Xuan-Hao Liu, Guang-Rui Wang, Nian‐Nian Zhong

et al.

npj Precision Oncology, Journal Year: 2025, Volume and Issue: 9(1)

Published: March 29, 2025

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, significantly impacting patient survival quality of life. The recent emergence immunotherapy has provided new hope for HNSCC patients, improving rates; however, only 15%–20% patients benefit, side effects are inevitable. With advancements in omics technologies growing prevalence bioinformatics research, immune microenvironment become increasingly well understood, molecular mechanisms underlying responses continue to be elucidated. In this review, we summarize commonly used techniques their applications research immunotherapy, including predicting enhancing efficacy, formulating personalized treatment plans, establishing robust preclinical models, identifying targets. Finally, explore future perspective terms sequencing samples, data integration analysis, emerging technologies, clinicopathological features, interdisciplinary approaches.

Language: Английский

Citations

0
Advancing precision medicine: strategies from organoids research DOI
Chenzhong Li,

Qionglin Liang,

Zhong Li

et al.

Cell organoid (Print), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Unveiling the functional roles of patient‐derived tumour organoids in assessing the tumour microenvironment and immunotherapy DOI Creative Commons
Di Chen, Lixia Xu,

Mengjuan Xuan

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(9)

Published: Sept. 1, 2024

Language: Английский

Citations

3
Tumor microenvironment and cancer metastasis: molecular mechanisms and therapeutic implications DOI Creative Commons
Çıgır Biray Avci, Bakiye Göker Bağca, Masoud Nikanfar

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 12, 2024

The tumor microenvironment (TME) plays a crucial role in cancer development and metastasis. This review summarizes the current research on how TME promotes metastasis through molecular pathways, focusing key components, such as cancer-associated fibroblasts, immune cells, endothelial cytokines, extracellular matrix. Significant findings have highlighted that alterations cellular communication within enable cells to evade surveillance, survive, invade other tissues. highlights roles of TGF-β VEGF signaling promoting angiogenesis matrix remodeling, which facilitate Additionally, we explored metabolic reprogramming stromal influenced by nutrient availability TME, drives progression. study also evaluated therapeutic strategies targeting these interactions disrupt By providing multidisciplinary perspective, this suggests understanding basis can lead more effective therapies identify potential avenues for future research. Future should prioritize unraveling complex environment, could novel personalized treatments. Moreover, advancements technologies single-cell analysis, spatial transcriptomics, epigenetic profiling offer promising identifying new targets improving efficacy immunotherapies, particularly context

Language: Английский

Citations

3
Generation of patient-derived glioblastoma organoids: a comparative study of enzymatic digestion and mechanical fragmentation methods DOI
Jian Zhang, Jiping Liu,

Yanghua Shi

et al.

Cell organoid (Print), Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Language: Английский

Citations

2