A C2H2-type zinc finger protein TaZFP8-5B negatively regulates disease resistance DOI Creative Commons
Lin Huang, R. Xie,

Yanling Hu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 23, 2024

Zinc finger proteins (ZFPs) are important regulators in abiotic and biotic stress tolerance plants. However, the role of ZFPs wheat responding to pathogen infection is poorly understood. In this study, we found TaZFP8-5B was down-regulated by Puccinia striiformis f. sp. tritici (Pst) infection. possesses a single C2H2-type zinc domain with plant-specific QALGGH motif, an EAR motif (LxLxL) at C-terminus. The represses trans-activation ability TaZFP8-5B. Knocking down expression TaZFP8 virus-induced gene silencing increased resistance Pst, whereas TaZFP8-5B-overexpressing reduced stripe rust rice Magnaporthe oryzae, suggesting that plays negative modulation plant immunity. Using bimolecular fluorescence complementation, split-luciferase, yeast two-hybrid assays, showed interacted calmodulin-like protein TaCML21. Knock-down TaCML21 Pst. This study characterized function its interacting Our findings provide new perspective on regulatory module made up TaCML21-TaZFP8-5B

Language: Английский

Powdery mildew effectors AVRA1 and BEC1016 target the ER J‐domain protein HvERdj3B required for immunity in barley DOI Creative Commons
Zizhang Li, Valeria Velásquez‐Zapata, J. Mitch Elmore

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(5)

Published: May 1, 2024

The barley powdery mildew fungus, Blumeria hordei (Bh), secretes hundreds of candidate secreted effector proteins (CSEPs) to facilitate pathogen infection and colonization. One these, CSEP0008, is directly recognized by the nucleotide-binding leucine-rich-repeat (NLR) receptor MLA1 therefore designated AVR

Language: Английский

Citations

7
Functional characterization of four soybean C2H2 zinc-finger genes in Phytophthora resistance DOI Creative Commons
Yuting Chen, Xinyue Liu,

Yanyan Zhou

et al.

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 20, 2025

Soybean (Glycine max) is one of the most important industrial and oilseed crops; however, yield threatened by invasion various pathogens. stem root rot, caused Phytophthora sojae, a destructive disease that significantly damages soybean production worldwide. C2H2 zinc finger protein (C2H2-ZFP) large transcription factor family in plants plays crucial roles stress response hormone signal transduction. Given its importance, we analyzed expression patterns C2H2-ZFP genes to P. sojae infection selected four candidate explore their molecular characteristics functions related resistance. Subcellular localization analysis indicated three ZFPs (GmZFP2, GmZFP3, GmZFP4) were localized nucleus, while GmZFP1 was found both nucleus plasma membrane. Dual-luciferase transient revealed all possessed transcriptional repression activation. Further N. benthamiana leaves demonstrated GmZFP2 induced significant cell death reactive oxygen species (ROS) accumulation. enhanced resistance pathogens hairy roots. This study provides insights functional characterization demonstrates positive role soybeans.

Language: Английский

Citations

0
Population genomics and molecular epidemiology of wheat powdery mildew in Europe DOI Creative Commons

Jigisha Jigisha,

Jeanine Ly,

Nikolaos Minadakis

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(5), P. e3003097 - e3003097

Published: May 2, 2025

Agricultural diseases are a major threat to sustainable food production. Yet, for many pathogens we know exceptionally little about their epidemiological and population dynamics, this knowledge gap is slowing the development of efficient control strategies. Here study genomics molecular epidemiology wheat powdery mildew, disease caused by biotrophic fungus Blumeria graminis forma specialis tritici (Bgt). We sampled Bgt across two consecutive years, 2022 2023, compiled genomic dataset 415 isolates from 22 countries in Europe surrounding regions. identified single epidemic unit north Europe, consisting highly homogeneous population. Conversely, south hosts smaller local populations which less interconnected. In addition, show that structure can be largely predicted prevalent wind patterns. several loci were under selection recent past, including fungicide targets avirulence genes. Some these common between populations, while others not, suggesting different selective pressures. reconstructed evolutionary history one loci, AvrPm17 , coding an effector recognized receptor Pm17. found evidence soft sweep on standing genetic variation. Multiple haplotypes, partially escape recognition Pm17, spread rapidly throughout continent upon its introduction early 2000s. also new virulent variant, emerged more recently evade Pm17 resistance altogether. Overall, highlight potential surveillance resolving dynamics agricultural pathogens, as well guiding

Language: Английский

Citations

0
An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew DOI Creative Commons
Chao Ma,

Xiubin Tian,

Zhenjie Dong

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

Citations

3
The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum–Plasma Membrane Contact Sites and Interacts With AVRPM3b2/c2 Through Its LRR Domain DOI Creative Commons
Jonatan Isaksson, Lukas Kunz, Simon Flueckiger

et al.

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 1, 2025

ABSTRACT Plant nucleotide‐binding leucine‐rich repeat (NLR) proteins are intracellular immune receptors that directly or indirectly perceive pathogen‐derived effector to induce an response. NLRs display diverse subcellular localisations, which associated with the capacity of receptor confer disease resistance and recognise its corresponding avirulence effector. In wheat, NLR PM3b recognises wheat powdery mildew AVRPM3 b2/c2 we examined molecular mechanism underlying this recognition. We show other PM3 variants localise endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (EPCS), while localises nucleocytoplasmic space. Additionally, found interacts in planta through LRR domain. further demonstrate full‐length interaction is considerably weaker than for isolated domain susceptible variant PM3CS, indicating activation leads dissociation complex. line this, observed a strong between P‐loop mutant was unable initiate cell death response, when inactive used. propose transiently residues conserved among variants, amino acids necessary full signalling unique PM3b. Our data suggests localisation differ from well‐studied highlights mechanistic diversity NLR‐mediated responses against pathogens plants.

Language: Английский

Citations

0
The barley MLA13-AVRA13 heterodimer reveals principles for immunoreceptor recognition of RNase-like powdery mildew effectors DOI Creative Commons
Aaron W. Lawson, Andrea Flores-Ibarra, Yu Cao

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Abstract Co-evolution between cereals and pathogenic grass powdery mildew fungi is exemplified by sequence diversification of an allelic series barley resistance genes encoding Mildew Locus A (MLA) nucleotide-binding leucine-rich repeat (NLR) immunoreceptors with N-terminal coiled-coil domain (CNLs). Each immunoreceptor recognises a matching, strain-specific effector encoded avirulence gene ( AVR ). We present here the cryo-EM structure MLA13 in complex its cognate A13 -1. The adopts RNase-like fold when bound to planta, similar crystal structures other effectors unbound receptors. -1 interacts via basal loops C-terminal repeats (LRRs) central winged helix (WHD). Co-expression structure-guided substitution variants show that receptor–effector interface plays essential role mediating immunity-associated plant cell death. Furthermore, combining structural information from MLA13–AVR heterocomplex alignments MLA receptors, we engineered single amino acid MLA7 enables expanded detection virulent variant -V2. In contrast pentameric conformation previously reported effector-activated CNL resistosomes, was purified resolved as stable heterodimer planta expression system. Our study suggests common principle for binding MLAs highlights utility engineering immune receptors broadening their pathogen recognition capabilities.

Language: Английский

Citations

0
TaANK-TPR1 enhances wheat resistance against stripe rust via controlling gene expression and protein activity of NLR protein TaRPP13L1 DOI
Shuangyuan Guo, Feng Zhang, Xiaona Du

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Development and application of the GenoBaits®WheatSNP16K array to accelerate wheat genetic research and breeding DOI Creative Commons
Shengjie Liu,

Mingjie Xiang,

Xiaoting Wang

et al.

Plant Communications, Journal Year: 2024, Volume and Issue: 6(1), P. 101138 - 101138

Published: Sept. 24, 2024

Language: Английский

Citations

2
Analysis of a global wheat panel reveals a highly diverse introgression landscape and provides evidence for inter-homoeologue chromosomal recombination DOI Creative Commons
Matthias Heuberger, Zoe Bernasconi, Mahmoud Said

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(10)

Published: Sept. 28, 2024

Language: Английский

Citations

2
Population genomics and molecular epidemiology of wheat powdery mildew in Europe DOI Creative Commons

Jigisha Jigisha,

Jeanine Ly,

Nikolaos Minadakis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract Agricultural diseases are a major threat to sustainable food production. Yet, for many pathogens we know exceptionally little about their epidemiological and population dynamics, this knowledge gap is slowing the development of efficient control strategies. Here study genomics molecular epidemiology wheat powdery mildew, disease caused by biotrophic fungus Blumeria graminis forma specialis tritici (Bgt). We sampled Bgt two consecutive years, 2022 2023, from 22 countries in Europe surrounding regions, compiled genomic dataset 415 isolates. found one single epidemic unit north Europe, consisting highly homogeneous population. Conversely, south hosts smaller local populations which less interconnected. In addition, show that structure can be largely predicted prevalent wind patterns. identified several loci were under selection recent past, including fungicide targets avirulence genes. Some these common between populations, while others not, suggesting different selective pressures. reconstructed evolutionary history loci, AvrPm17 , coding an effector recognized receptor Pm17. evidence soft sweep on standing genetic variation. Multiple haplotypes, partially escape recognition Pm17, spread rapidly throughout continent upon its introduction early 2000s. also new virulent variant, emerged more recently evade Pm17 resistance altogether. Overall, highlight potential surveillance resolving dynamics agricultural pathogens, as well guiding

Language: Английский

Citations

2