Transwell-based microphysiological platform for high-resolution imaging of airway tissues DOI Open Access
Amanzhol Kurmashev, Julia Alicia Boos, Benoît‐Joseph Laventie

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 22, 2023

Abstract Transwell-based airway models have become increasingly important to study the effects of respiratory diseases and drug treatment at air-liquid interface lung epithelial barrier. However, underlying mechanisms tissue cell level often remain unclear, as transwell inserts feature limited live-cell imaging compatibility. Here, we report on a novel microphysiological platform for cultivation transwell-based tissues providing possibility alternate between liquid-liquid interfaces. While recapitulates physiological conditions model, enables live-imaging high spatiotemporal resolution. The plastics-based microfluidic insertion recuperation inserts, which allows analysis under standardized well plate conditions. We used device monitor infections Pseudomonas aeruginosa in human stem-cell-derived bronchial tissue. continuously imaged progression P. infection real time resolution, provided insights into bacterial spreading invasion apical surface, breaching destruction over time. culture system is powerful tool visualize elucidate key processes developing facilitate testing development.

Language: Английский

Recent frontiers in biofabrication for respiratory tissue engineering DOI Creative Commons
Amanda Zimmerling, Nuraina Anisa Dahlan,

Yan Zhou

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 40, P. e00342 - e00342

Published: April 21, 2024

Respiratory tissue engineering offers a robust framework for studying cell-cell and host-pathogen interactions in tissue-like environment platform lung regeneration disease mechanisms. However, the challenge of replicating dynamic three-dimensional (3D) microenvironments is huge obstacle with existing technology. Current animal models two-dimensional cell culture do not replicate vivo conditions seen human lungs, thus research utilizing these techniques often fails to help alleviate global burden respiratory diseases. has been drawing significant attention over past decade. Particularly emergence severe acute syndrome coronavirus 2 (SARS-CoV-2), many inspiring developments advances have reported. This review presents recent focusing on 3D bioprinting, organ-on-a-chip, organoid technologies. It also provides an overview attempts integrate biomechanical stimulus aim improving integrity constructs enhancing cellular propagation. addresses challenges inherent discusses future prospects this field, urging continuing innovation investment toward success increasing clinical relevance.

Language: Английский

Citations

5
Ultraviolet germicidal irradiation: Advances in viral inactivation and vaccine development DOI Creative Commons
Kathleen K. M. Glover, Sunday S. Nunayon, Lexuan Zhong

et al.

Indoor Environments, Journal Year: 2025, Volume and Issue: unknown, P. 100099 - 100099

Published: May 1, 2025

Citations

0
Multiomic Investigations into Lung Health and Disease DOI Creative Commons
Sarah E. Blutt, Cristian Coarfa, Josef Neu

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 2116 - 2116

Published: Aug. 19, 2023

Diseases of the lung account for more than 5 million deaths worldwide and are a healthcare burden. Improving clinical outcomes, including mortality quality life, involves holistic understanding disease, which can be provided by integration multi-omics data. An enhanced comprehensive multiomic datasets provides opportunities to leverage those inform treatment prevention diseases classifying severity, prognostication, discovery biomarkers. The main objective this review is summarize use multiomics investigations in machine learning computational methods. This also discusses disease models, animal organoids, single-cell lines, study health disease. We provide examples where have deeper insight into etiopathogenesis resulted improved preventative therapeutic interventions.

Language: Английский

Citations

6
Multiomic Investigations into Lung Health and Disease DOI Open Access
Sarah E. Blutt, Cristian Coarfa, Josef Neu

et al.

Published: May 9, 2023

Diseases of the lung account for more than 5 million deaths worldwide and are a burden to healthcare. Improving clinical outcomes including mortality quality life involves holistic understanding etiopathogenesis, which can be provided by multi-omics integration data. An enhanced large comprehensive datasets provides opportunities mine those features that contribute prevention amelioration disease. In this review, we evaluate disease models animal models, organoids single cell lines as mechanisms study multiomics in health We provide examples diseases where investigations have deeper insight into pathogenesis has resulted improved preventive therapeutic interventions.

Language: Английский

Citations

4
A prospects tool in virus research: Analyzing the applications of organoids in virus studies DOI
Qi Shen, Yuhan Zhou, Yanqiu Zhou

et al.

Acta Tropica, Journal Year: 2024, Volume and Issue: 254, P. 107182 - 107182

Published: March 11, 2024

Language: Английский

Citations

1
Transwell‐Based Microfluidic Platform for High‐Resolution Imaging of Airway Tissues DOI Creative Commons
Amanzhol Kurmashev, Julia Alicia Boos, Benoît‐Joseph Laventie

et al.

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: 9(20)

Published: July 10, 2024

Abstract Transwell‐based airway models have become increasingly important in studying the effects of respiratory diseases and drug treatment at air–liquid interface lung epithelial barrier. However, underlying mechanisms tissue cell level often remain unclear, as transwell inserts feature limited live‐cell imaging compatibility. Here, a novel microfluidic platform is reported for cultivation transwell‐based tissues providing possibility to alternate between liquid–liquid interfaces. While recapitulates physiological conditions model, enables live high spatiotemporal resolution. The plastics‐based insertion recuperation inserts, which allows analysis under standardized well plate conditions. device used monitor infections Pseudomonas aeruginosa human stem‐cell‐derived bronchial tissue. progression P. infection real‐time resolution continuously imaged, provides insights into bacterial spreading invasion on apical surface, breaching destruction over time. culture system powerful tool visualize elucidate key processes developing facilitate testing development.

Language: Английский

Citations

1
Selection of the Most Suitable Culture Medium for Patient-Derived Lung Cancer Organoids DOI Creative Commons
Melissa Acosta-Plasencia, Yangyi He, Daniel Martı́nez

et al.

Cells Tissues Organs, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Sept. 5, 2024

Introduction: Patient-derived organoids have emerged as a promising in vitro model for precision medicine, particularly cancer, but also noncancer-related diseases. However, the optimal culture medium culturing patient-derived lung has not yet been agreed upon. This study aimed to shed light on selection of media developing studies using organoids. Methods: Tumor and normal paired tissue from 71 resected non-small cell cancer patients were processed organoid culture. Lung (LCOs) derived tumor (LNOs) nonneoplastic tissue. Three different compared: permissive (PCM), limited (LCM), minimum basal (MBM). We assessed their effectiveness establishing cultures, promoting growth viability, compared differential phenotypic characteristics. Results: While PCM was associated with highest success rate useful long-term expansion, MBM best option avoid overgrowth The density, size, viability LNOs reduced LCM severely affected MBM. cultured tend differentiate bronchospheres, while alveolosphere differentiation can be observed those LCM. morphological phenotype LCO influenced by election. Mesenchymal when used. Conclusion: work highlights importance considering research objectives selecting most suitable growing

Language: Английский

Citations

1
Stem Cells/Lung Organoids and Lung Rejuvenation in the Treatment of Chronic Obstructive Pulmonary Disease and Pulmonary Emphysema DOI
Francesco Nucera, Philip M. Hansbro, Lauren H. Fairley

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 94 - 114

Published: Jan. 1, 2024

Language: Английский

Citations

0
Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infection DOI Creative Commons
Shashi Kant Tiwari, Tariq M. Rana

Heliyon, Journal Year: 2023, Volume and Issue: 9(9), P. e19601 - e19601

Published: Aug. 29, 2023

The lack of physiologically relevant in vitro models has hampered progress understanding human lung development and disease. Here, we describe a protocol which induced pluripotent stem cells (hiPSCs) undergo stepwise differentiation into definitive endoderm (>88% population) to three-dimensional (3D) organoids (LORGs), contain both epithelial mesenchymal cellular architecture display proximal distal airway patterning. These LORGs can maintained for more than 90 days by re-embedding the Matrigel. We show utility disease modeling drug screening infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) treatment antiviral drugs.

Language: Английский

Citations

1
Organoids in COVID-19: can we break the glass ceiling? DOI Creative Commons
Chiu Wang Chau, Ryohichi Sugimura

Journal of Leukocyte Biology, Journal Year: 2023, Volume and Issue: 115(1), P. 85 - 99

Published: Aug. 24, 2023

COVID-19 emerged in September 2020 as a disease caused by the virus SARS-CoV-2. The presented pneumonia at first but later was shown to cause multisystem infections and long-term complications. Many efforts have been put into discovering exact pathogenesis of disease. In this review, we aim discuss an emerging tool modeling, organoids, investigation COVID-19. This review will introduce some methods breakthroughs achieved organoids limitations system.

Language: Английский

Citations

0