Responses of adult crayfish to macro-nutrients intake alteration during juvenile stage on metabolism and intestinal microbiota DOI Creative Commons
Yan Shui, Shengyan Su,

Zenghong Xu

et al.

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 34, P. 101896 - 101896

Published: Dec. 22, 2023

Red swamp crayfish Procambarus clarkii is becoming an ecologically and economically important crustacean species in China. In present study, whether the macro-nutrients intake intervention during early life resulting long-term influences on nutritional use metabolism were evaluated view of concept programming effects. Juvenile underwent a 14 days high-carbohydrate (43%) low-protein (17%) stimulus, following 70 routine dietary feeding (carbohydrate 15%, protein 36%), until adulthood. Short- (14 days) (84 effects respectively terms growth performances, digestive enzymes activities, body compositions, intestinal microbiota (long-term only). Data showed that short term, it enhanced activities amylase lipase but reduced activity trypsase hepatopancreas. long decreased performances (SR WGR) modified diversity obviously (p < 0.05). Throughout period increased crude level. All results indicated events caused impacts nutrient thus affect physiology short, work provided evidences to support existence juvenile crayfish.

Language: Английский

A first glimpse into the m6A modification machinery of shrimp: Genomic features, expression patterns and potential roles in molting regulation DOI Creative Commons

Yiguo Lei,

Qingtian Zeng,

Guangsheng Tan

et al.

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 29, P. 101493 - 101493

Published: Feb. 9, 2023

N6-methyladenosine (m6A) is the most abundant type of RNA modification in eukaryotes, yet information on m6A methylation enzymatic machinery decapod crustaceans quite scarce. Here, three essential genes for modification, including two methyltransferases (LvMETTL3 and LvWTAP) a m6A-binding protein (LvYTHDF2), were identified from Pacific whiteleg shrimp Litopenaeus vannamei. Their genomic structures dissected an evolutionary context. Common transcription factor binding sites conserved functional motifs identified, but also possessed some unique features, illustrating lineage-specific diversification regulatory elements functions these genes. The displayed ubiquitous tissue expression profiles, showed dynamic changes at different molting stages. Following suppression LvMETTL3, LvWTAP or LvYTHDF2 by interference, rate was significantly accelerated, molt inhibiting hormone MIH plummeted, while major ecdysone signaling pathway EcR, RXR E75 all significant induction. results indicated that may regulate process via pathway. current study provided first molecular characterization several key components penaeid shrimp, established foundation further exploration roles development growth.

Language: Английский

Citations

7
Integration of transcriptome and whole-genome re-sequencing analyses reveal growth-related candidate genes in Procambarus clarkii DOI

Zihao Zhou,

Yanhe Li, Yu‐Dong Shen

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 49, P. 101198 - 101198

Published: Feb. 1, 2024

Language: Английский

Citations

2
Folic acid improves the growth and immune response of crayfish (Procambius clarkii) by regulating glucose metabolism DOI Creative Commons
Xiaona Xu,

Shuangshuang Ping,

Fen Wang

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 36, P. 102077 - 102077

Published: April 15, 2024

Folic acid (FA) is an essential vitamin for the growth and health of aquatic organisms in aquaculture industry. The purpose this study was to investigate effects potential mechanisms action dietary FA on immune response red swamp crayfish (Procambius clarkii). Crayfish (n = 360; 3 tanks 20 each treatment) were fed diets containing different levels (0 [control], 0.5, 1, 2, 4, 8 mg/kg dry feed weight) 60 d. Broken-line analysis based final weight specific rate revealed that optimal level promoting 2 mg/kg. Villus length, lipase activity, antioxidant superoxide dismutase activity significantly increased under treatment. Transcriptome identified 177 upregulated 298 downregulated differentially expressed genes (DEGs) between 0 (control) groups. Gene Ontology Kyoto Encyclopedia Genes Genomes analyses showed most enriched pathways related glucose metabolism. Among DEGs involved metabolism, Tpi1 (triosephosphate isomerase 1) Pck1 (phosphoenolpyruvate carboxykinase treatment, respectively. Our findings suggest requirement may enhance nutrient bioavailability regulate metabolism improve performance.

Language: Английский

Citations

2
Genome-wide investigation of toll-like receptor genes (TLRs) in Procambarus clarkia and their expression pattern in response to black may disease DOI

Haifu Wan,

Shumei Mu,

Baohua Duan

et al.

Fish & Shellfish Immunology, Journal Year: 2022, Volume and Issue: 131, P. 775 - 784

Published: Nov. 2, 2022

Language: Английский

Citations

12
Insights into chromosomal evolution and sex determination of Pseudobagrus ussuriensis (Bagridae, Siluriformes) based on a chromosome-level genome DOI Creative Commons
Chuankun Zhu, Haiyang Liu, Zhengjun Pan

et al.

DNA Research, Journal Year: 2022, Volume and Issue: 29(4)

Published: June 25, 2022

Pseudobagrus ussuriensis is an aquaculture catfish with significant sexual dimorphism. In this study, a chromosome-level genome size of 741.97 Mb was assembled for female P. ussuriensis. A total 26 contigs covering 97.34% the whole-genome assembly were obtained N50 28.53 and L50 11. 24,075 protein-coding genes identified, 91.54% (22,039) being functionally annotated. Based on assembly, four chromosome evolution clusters catfishes identified formation process chromosomes predicted. 55 sex-related quantitative trait loci (QTLs) phenotypic variance explained value 100% located 8 (chr08). The QTLs other previously sex-specific markers in sex-determining region 16.83 (from 6.90 to 23.73 Mb) chr08, which predicted as X chromosome. comprised 554 genes, 135 differently expressed between males females/pseudofemales, 16 candidate screened out. results study provided useful genetic, genomic evolutionary studies ussuriensis, also be further sex-determination mechanism analysis sex-control breeding fish.

Language: Английский

Citations

9
Whole genome evaluation analysis and preliminary Assembly of Oratosquilla oratoria (Stomatopoda: Squillidae) DOI

Xiaoli Sun,

Gang Wang, Jie Yang

et al.

Molecular Biology Reports, Journal Year: 2023, Volume and Issue: 50(5), P. 4165 - 4173

Published: March 9, 2023

Language: Английский

Citations

5
The genome of a sea spider corroborates a shared Hox cluster motif in arthropods with reduced posterior tagma DOI Creative Commons
Νικόλαος Παπαδόπουλος, Siddharth Kulkarni, Christian Baranyi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Background: Chelicerate evolution is contentiously debated, with recent studies challenging traditional phylogenetic hypotheses and scenarios of major evolutionary events, like terrestrialization. Sea spiders (Pycnogonida) represent the uncontested marine sister group all other chelicerates, featuring a - likely plesiomorphic indirect development mode. Accordingly, pycnogonids hold potential to provide crucial insight into early chelicerate genome ancestral principles body patterning. Leveraging this potential, however, has hitherto been hampered by lack high-quality genomic resources for pycnogonids. Results: We employed long-read sequencing proximity ligation data assemble first near chromosome-level sea spider Pycnogonum litorale, complemented developmental transcriptomes high-fidelity Iso-Seq dataset. The assembly size 471Mb in 57 pseudochromosomes, repeat content 61.05%, 15,497 predicted protein-coding genes, highly complete (95.8% BUSCO Arthropoda score, 95.7% conserved microRNA families present). identified single, intact Hox gene cluster that lacks abdominal-A/Hox9, suggesting loss gene. Conclusions: Our transcriptomic establish P. litorale as key research organism modern on evolution, development, phylogeny. presence single further strengthens inference no whole-genome duplication occurred at base tree. abdA suggests combination posterior tagmata reduction common theme arthropod it shared other, distantly related taxa vestigial opisthosoma/abdomen.

Language: Английский

Citations

1
Full-Length Transcriptome of Red Swamp Crayfish Hepatopancreas Reveals Candidate Genes in Hif-1 and Antioxidant Pathways in Response to Hypoxia-Reoxygenation DOI
Yu Xu, Hai Lin,

Weihui Yan

et al.

Marine Biotechnology, Journal Year: 2022, Volume and Issue: 24(1), P. 55 - 67

Published: Jan. 8, 2022

Language: Английский

Citations

7
Identification of quantitative trait loci for growth traits in red swamp crayfish (Procambarus clarkii) DOI Creative Commons

Junxiao Sun,

Cuirong Luo,

Bo Peng

et al.

Aquaculture and Fisheries, Journal Year: 2023, Volume and Issue: 8(6), P. 727 - 736

Published: Jan. 21, 2023

Genetic breeding is an important approach to improve the economic traits of aquaculture animals. Unfortunately, genetic basis for red swamp crayfish, including growth-related traits, has not yet been reported. In present study, two full-sib families and natural populations were employed identify quantitative trait loci (QTLs) crayfish. total, 28 QTLs five body weight, length, carapace width, abdomen repeatedly identified in more than families/populations, by means association analysis; nine these displayed a heterosis effect. The phenotypic variations investigated explained ranged from 4.2% 19.0%. Eight additive three further successfully validated using comparative analysis favoured alleles between small big size crayfish groups population. conclusion, this study which great significance their application future molecular species.

Language: Английский

Citations

3
Abundance and Diversification of Repetitive Elements in Decapoda Genomes DOI Open Access
Christelle Rutz, Lena Bonassin, Arnaud Kress

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(8), P. 1627 - 1627

Published: Aug. 15, 2023

Repetitive elements are a major component of DNA sequences due to their ability propagate through the genome. Characterization Metazoan repetitive profiles is improving; however, current pipelines fail identify significant proportion divergent repeats in non-model organisms. The Decapoda order, for which repeat content analyses largely lacking, characterized by extremely variable genome sizes that suggest an important presence elements. Here, we developed new standardized pipeline annotate organisms, applied twenty and six other Crustacea genomes. Using this tool, identified 10% more than standard pipelines. were abundant species Crustacea, with very large number highly repeated satellite families. Moreover, demonstrated high correlation between assembly size transposable different dynamics Dendrobranchiata Reptantia. patterns reflect phylogenetic relationships distinct evolutionary trajectories within Crustacea. In summary, our results highlight impact on evolution value novel annotation pipeline, will provide baseline future comparative analyses.

Language: Английский

Citations

3