Where Biology Meets Engineering: Scaling Up Microbial Nutraceuticals to Bridge Nutrition, Therapeutics, and Global Impact

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 566 - 566

Published: March 2, 2025

The global nutraceutical industry is experiencing a paradigm shift, driven by an increasing demand for functional foods and dietary supplements that address malnutrition chronic diseases such as obesity, diabetes, cardiovascular conditions, cancer. Traditional plant- animal-derived nutraceuticals face limitations in scalability, cost, environmental impact, paving the way microbial biotechnology sustainable alternative. Microbial cells act bio-factories, converting nutrients like glucose amino acids into valuable products polyunsaturated fatty (PUFAs), peptides, other bioactive compounds. By harnessing their natural metabolic capabilities, microorganisms efficiently synthesize these compounds, making production effective approach development. This review explores transformative role of platforms nutraceuticals, emphasizing advanced fermentation techniques, synthetic biology, engineering. It addresses challenges optimizing strains, ensuring product quality, scaling while navigating regulatory frameworks. Furthermore, highlights cutting-edge technologies CRISPR/Cas9 genome editing, adaptive evolution strain enhancement, bioreactor innovations to enhance yield efficiency. With focus on sustainability precision, positioned game-changer industry, offering eco-friendly scalable solutions meet health needs. integration omics exploration novel sources hold potential revolutionize this field, aligning with growing consumer innovative products.

Language: Английский

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Recent progress in adaptive laboratory evolution of industrial microorganisms

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2022, Volume and Issue: 50(1)

Published: Nov. 2, 2022

Adaptive laboratory evolution (ALE) is a technique for the selection of strains with better phenotypes by long-term culture under specific pressure or growth environment. Because ALE does not require detailed knowledge variety complex and interactive metabolic networks, only needs to simulate natural environmental conditions in design pressure, it has advantages broad adaptability, strong practicability, more convenient transformation strains. In addition, provides powerful method studying evolutionary forces that change phenotype, performance, stability strains, resulting productive industrial beneficial mutations. recent years, been widely used activation microbial pathways phenotypic optimization, efficient utilization substrates, optimization tolerance toxic substance, biosynthesis target products, which conducive production excellent characteristics. this paper, typical examples applications development research progress technology are reviewed, followed discussion its prospects.

Language: Английский

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Effect of adaptive laboratory evolution of engineered Escherichia coli in acetate on the biosynthesis of succinic acid from glucose in two-stage cultivation

Bioresources and Bioprocessing, Journal Year: 2024, Volume and Issue: 11(1)

Published: April 5, 2024

Abstract Escherichia coli MLB (MG1655 Δ pflB ldhA ), which can hardly grow on glucose with little succinate accumulation under anaerobic conditions. Two-stage fermentation is a in the first stage used for cell growth and second product production. The ability of consumption production conditions be improved significantly by using acetate as solo carbon source aerobic condition during two-stage fermentation. Then, adaptive laboratory evolution (ALE) growing was applied here. We assumed that activities related enzymes might further this study. E. MLB46-05 evolved from it had an phenotype acetate. Interestingly, MLB46-05, yield tolerance succinic acid were significantly. According to transcriptome analysis, upregulation glyoxylate cycle activity stress regulatory factors are possible reasons elevated yield. And increased made more tolerant high concentrations succinate. Finally, strain produced 111 g/L 0.74 g/g glucose. Synopsis

Language: Английский

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Adaptive Laboratory Evolution of Microorganisms: Methodology and Application for Bioproduction

Microorganisms, Journal Year: 2022, Volume and Issue: 11(1), P. 92 - 92

Published: Dec. 29, 2022

Adaptive laboratory evolution (ALE) is a useful experimental methodology for fundamental scientific research and industrial applications to create microbial cell factories. By using ALE, cells are adapted the environment that researchers set based on their objectives through serial transfer of populations in batch cultivations or continuous cultures fitness (i.e., growth) under such an increases. Then, omics analyses evolved mutants, including genome sequencing, transcriptome, proteome metabolome analyses, performed. It expected can understand evolutionary adaptation processes, applications, microorganisms exhibit increased carbon source availability, stress tolerance, production target compounds analysis data. In this review article, methodologies ALE introduced. Moreover, application creation as factories has also been

Language: Английский

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Enhanced fermentative γ-aminobutyric acid production by a metabolic engineered Corynebacterium glutamicum

Biotechnology and Bioprocess Engineering, Journal Year: 2024, Volume and Issue: 29(1), P. 129 - 140

Published: Feb. 1, 2024

Language: Английский

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Balancing the (R)-pantoate and β-alanine pathway of Escherichia coli by modular co-culture engineering to produce D-pantothenic acid from glycerol

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105964 - 105964

Published: Jan. 1, 2025

Language: Английский

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Isolation of derivatives from the food-grade probiotic Lactobacillus johnsonii CNCM I-4884 with enhanced anti- Giardia activity

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: March 27, 2025

Giardiasis, a widespread intestinal parasitosis affecting humans and animals, is growing concern due to the emergence of drug-resistant strains G. intestinalis. Probiotics offer promising alternative for preventing treating giardiasis. Recent studies have shown that probiotic Lactobacillus johnsonii CNCM I-4884 inhibits intestinalis growth both in vitro vivo. This protective effect largely mediated by bile salt hydrolase (BSH) enzymes, which convert conjugated acids (BAs) into free forms are toxic parasite. The objective this study was use adaptive evolution develop stress-resistant derivatives L. I-4884, with aim improving its anti-Giardia activity. Twelve enhanced resistance BAs reduced autolysis were generated. Among them, derivative M11 exhibited highest BSH Genomic proteomic analyses revealed two SNPs upregulation global stress response SigB, likely contributed increased overproduction. Finally, efficacy validated murine model In conclusion, our results demonstrate an effective strategy generate robust food-grade bacteria improved health benefits.

Language: Английский

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Adaptive evolution and reverse engineering to explore the low pH tolerance mechanisms of Streptomyces albulus

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Streptomyces albulus is well-known as a cell factory for producing ε-poly-L-lysine (ε-PL), but its ability to produce effectively requires an environment with pH of about 4.0. Unfortunately, prolonged exposure low compromises the cellular integrity S. albulus, leading decrease in efficiency ε-PL biosynthesis. To enhance tolerance and investigate mechanisms, we employed adaptive laboratory evolution (ALE) technology evolve GS114 strain by progressively lowering environmental pH. This process ultimately yielded mutant ALE3.6, which exhibited significantly improved at 3.6 achieved 37.9% increase production compared parental under optimal fermentation condition. The physiological evaluation ALE3.6 indicated pronounced enhancement membrane wall conditions. identify key genes involved tolerance, whole-genome resequencing quantitative real-time PCR, pinpointed desA, gatD, mamU critical contributors. We further validated roles these through reverse engineering, both efficiency. Finally, elucidated response mechanisms stress. study enhances understanding species, particularly regarding valuable biochemical products challenging conditions.IMPORTANCEIn this study, viability staged while simplifying previously studied fed-batch strategy. identified associated strains' phenotypes utilizing engineering. Subsequently,

Language: Английский

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Advances in engineering and applications of microbial glutamate decarboxylases for gamma-aminobutyric acid production

Enzyme and Microbial Technology, Journal Year: 2025, Volume and Issue: 188, P. 110652 - 110652

Published: April 6, 2025

Language: Английский

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Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in Escherichia Coli

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(11), P. 4656 - 4664

Published: March 7, 2023

γ-Aminobutyric acid (GABA) is used widely in various fields, such as agriculture, food, pharmaceuticals, and biobased chemicals. Based on glutamate decarboxylase (GadBM4) derived from our previous work, three mutants, GadM4-2, GadM4-8, GadM4-31, were obtained by integrating enzyme evolution high-throughput screening methods. The GABA productivity through whole-cell bioconversion using recombinant Escherichia coli cells harboring mutant GadBM4-2 was enhanced 20.27% compared to that of the original GadBM4. Further introduction central regulator GadE resistance system enzymes deoxyxylulose-5-phosphate-independent pyridoxal 5'-phosphate biosynthesis pathway resulted a 24.92% improvement productivity, reaching 76.70 g/L/h without any cofactor addition with greater than 99% conversion ratio. Finally, when one-step applied for catalysis 5 L bioreactor, titer reached 307.5 ± 5.94 g/L 61.49 crude l-glutamic (l-Glu) substrate. Thus, biocatalyst constructed above combined method represents an effective approach industrial production.

Language: Английский

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