Engineering carbon assimilation in plants

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Carbon assimilation is a crucial part of the photosynthetic process, wherein inorganic carbon, typically in form CO 2 , converted into organic compounds by living organisms, including plants, algae, and subset bacteria. Although several carbon fixation pathways have been elucidated, Calvin–Benson–Bassham (CBB) cycle remains fundamental to metabolism, playing pivotal role biosynthesis starch sucrose cyanobacteria. However, Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO), key carboxylase enzyme CBB cycle, exhibits low kinetic efficiency, substrate specificity, high temperature sensitivity, all which potential limit flux through this pathway. Consequently, RuBisCO needs be present at very concentrations, one factors contributing its status as most prevalent protein on Earth. Numerous attempts made optimize catalytic efficiency thereby promote plant growth. Furthermore, limitations process highlight benefits engineering or discovering more efficient mechanisms, either improving itself introducing alternative pathways. Here, we review advances artificial engineering, integration synthetic biology, genetic metabolic pathway optimization, intelligence order create plants capable performing photosynthesis. We additionally provide perspective current challenges solutions alongside personal opinion promising future directions emerging field.

Language: Английский

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C1 photochemotrophy – rethinking one-carbon metabolism in phototrophs

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Extracellular respiration is a latent energy metabolism in Escherichia coli

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Two highly specific growth-coupled biosensor for glycolaldehyde detection across micromolar and millimolar concentrations

Synthetic Biology, Journal Year: 2025, Volume and Issue: 10(1)

Published: Jan. 1, 2025

Abstract Glycolaldehyde (GA), the smallest sugar, has significant potential as a biomass-derived platform chemical and is key metabolite in several synthetic pathways for one-carbon metabolism new-to-nature photorespiration. This study introduces two metabolic schemes engineering Escherichia coli into GA biosensors. Through creating GA-dependent auxotrophies, we link growth of these strains to biosynthesis essential vitamin pyridoxal-5-phosphate, 2-ketoglutarate, respectively. We characterized optimized quantification externally added from 2 µM 1.5 mM. also demonstrate capability detect that produced intracellularly through different routes substrates such xylose, ethylene glycol, glycolate. Our biosensors offer complementary sensitivities features, opening up applications biology, which proof-of-principle by providing first vivo demonstration reduction glycolate route using engineered enzymes.

Language: Английский

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Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Auxotrophic metabolic sensors (AMS) are microbial strains modified so that biomass formation correlates with the availability of specific metabolites. These essential for bioengineering (e.g., in growth-coupled designs) but creating them is often a time-consuming and low-throughput process can be streamlined by silico analysis. Here, we present systematic workflow designing, implementing, testing versatile AMS based on Escherichia coli. Glyoxylate, key metabolite (synthetic) CO2 fixation carbon-conserving pathways, served as test analyte. Through iterative screening compact model, identify non-trivial designs result six wide sensitivity range glyoxylate, spanning three orders magnitude detected analyte concentration. We further adapt these E. coli sensing glycolate demonstrate their utility both pathway engineering (testing module carbon assimilation via glyoxylate) environmental monitoring (quantifying produced photosynthetic microalgae). Adapting this to different metabolites could facilitate design implementation diverse biotechnological applications.

Language: Английский

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Design and implementation of aerobic and ambient CO2-reduction as an entry-point for enhanced carbon fixation

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 1, 2025

Abstract The direct reduction of CO 2 into one-carbon molecules is key to highly efficient biological -fixation. However, this strategy currently restricted anaerobic organisms and low redox potentials. In study, we introduce the CORE cycle, a synthetic metabolic pathway that converts formate at aerobic conditions ambient levels, using only NADPH as reductant. Combining theoretical design analysis, enzyme bioprospecting high-throughput screening, modular assembly adaptive laboratory evolution, realize cycle in vivo demonstrate supports growth E. coli by supplementing C1-metabolism serine biosynthesis from . We further analyze potential new entry-point for carbon photorespiration autotrophy. Overall, our work expands solution space reduction, offering promising approach enhance fixation processes such photosynthesis, opening avenues

Language: Английский

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Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103195 - 103195

Published: Sept. 16, 2024

One-carbon (C1) feedstocks, such as carbon monoxide (CO), formate (HCO

Language: Английский

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Increasing Rubisco as a simple means to enhance photosynthesis and productivity now without lowering nitrogen use efficiency

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Summary Global demand for food may rise by 60% mid‐century. A central challenge is to meet this need using less land in a changing climate. Nearly all crop carbon assimilated through Rubisco, which catalytically slow, reactive with oxygen, and major component of leaf nitrogen. Developing more efficient forms or engineering CO 2 concentrating mechanisms into C 3 crops competitively repress oxygenation, are endeavors, could hugely increase photosynthetic productivity (≥ 60%). New technologies bringing closer, but improvements remain the discovery phase have not been reduced practice. simpler shorter‐term strategy that fill time gap, smaller increases ( c . 10%) Rubisco content. This has demonstrated initial field trials, improving 4 crops. Combining three‐dimensional canopies metabolic models infers 20% canopy photosynthesis 14% sugarcane (C ) 9% soybean ). consistent observed rice, maize, sorghum sugarcane. Upregulation calculated require nitrogen per unit yield although achieved transgenically date, might be gene editing produce transgene‐free gain function mutations breeding.

Language: Английский

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Extracellular Respiration is a Latent Energy Metabolism inEscherichia coli

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 3, 2024

ABSTRACT Diverse microbial species utilize redox shuttles to exchange electrons with their environment through mediated extracellular electron transfer (EET). This process maintains homeostasis and supports anaerobic survival across diverse communities. Although EET has been extensively leveraged for bioelectrocatalysis bioelectronics decades, fundamental questions remain about how these are reduced within cells bioenergetic implications. knowledge gap limits our understanding of the physiological roles in various microbes hampers development efficient electrochemical technologies. To address this, we developed a methodology integrating genome editing, electrochemistry, systems biology investigate mechanism implications bacteria. Using this approach, uncovered Escherichia coli . In absence alternative sinks, cycling 2-hydroxy-1,4-naphthoquinone (HNQ) via cytoplasmic nitroreductase enabled E. respire grow on an electrode. Genome-scale metabolic modeling suggested that HNQ- offers more energetically favorable route supporting growth than canonical fermentation. Transcriptome analysis revealed perturbations response HNQ identified rapid adaptations support growth. work demonstrates can independently classical transport chains fermentative pathways, unveiling new type energy metabolism.

Language: Английский

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Spiers Memorial Lecture: Engineering biocatalysts

Faraday Discussions, Journal Year: 2024, Volume and Issue: 252, P. 9 - 28

Published: Jan. 1, 2024

Enzymes are being engineered to catalyze chemical reactions for many practical applications in chemistry and biotechnology. The approaches used surveyed this short review, emphasizing methods accessing reactivities not expressed by native protein scaffolds. successful generation of completely

Language: Английский

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