Habitat correlates of cave-dwelling: A radiation-scale analysis of skin traits and comparative transcriptomics of theSinocyclocheiluscavefish DOI Creative Commons
X Luo, 陈兵 Chen Bing, Tingru Mao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 17, 2023

ABSTRACT With 78 species, Sinocyclocheilus cavefish constitute the largest radiation in world. They exhibit remarkable morphological diversity across three habitat types: surface (Surface morphs, Normal-eyed, variably colored), exclusively-cave-dwelling (Stygobitic Eyeless, depigmented), and intermediate between cave (Stygophilic Micro-eyed, partially depigmented). Distinctive traits of include variations eye skin conditions associated with their habitat, despite role sensing environmental changes, its correlates are less understood, compared to well-studied conditions. Here, we analyzed correlation morphology utilizing transcriptomics-based methods. We generated RNA-sequencing data for nine species integrated those existing from five additional species. These 14 represent primary clades major habitats these cavefish. Data on color scale were 7374 orthologous genes identified. Using a comparative transcriptomics approach, identified 1,348 differentially expressed (DEGs) morphotypes. GO KEGG enrichment analyses suggest that have evolved different strategies energy metabolism, immunity, oxidative stress habitats. also found 329 positive selection (PSGs) mainly involved apoptosis, necrosis, indicating potential adaptations The maximum likelihood phylogenetic tree, based 1369 single-copy was largely concordant currently established RAD-seq mt-DNA phylogenies, but few exceptions. Species higher dependence present lighter coloration, fewer dark blotches, diminished coverage. PCA cluster analysis suggested cave-dwelling characterized by absence black similar expression patterns, convergence adaptation. Variations tyrosine metabolism may explain pigmentation differences among diverse Our study highlights significance shaping variation, while offering insights into molecular mechanisms driving habitat-specific . findings underscore transcriptional variation adapting environments contribute future studies evolution ecology

Language: Английский

Species delimitation, discovery and conservation in a tiger beetle species complex despite discordant genetic data DOI Creative Commons
Daniel P. Duran, Robert Laroche, Stephen J. Roman

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 19, 2024

Abstract In an age of species declines, delineating and discovering biodiversity is critical for both taxonomic accuracy conservation. recent years, there has been a movement away from using exclusively morphological characters to delineate describe taxa increase in the use molecular markers diversity or through integrative taxonomy, which employs traditional characters, as well genetic other data. Tiger beetles are charismatic, conservation concern, much work done on delineation subspecies, but few these have tested with analyses. this study, we morphologically based hypotheses polymorphic tiger Eunota circumpicta (LaFerté-Sénectère, 1841) complex multilocus genomic mtDNA We find multiple cryptic within previous concept , some were historically recognized subspecies. found that datasets did not identify same units was most at odds all patterns. Overall, new diversity, raises important concerns, provide working example testing subspecies validity despite discordant

Language: Английский

Citations

4
Sinocyclocheilus longicornus (Cypriniformes, Cyprinidae), a new species of microphthalmic hypogean fish from Guizhou, Southwest China DOI Creative Commons
Cheng Xu, Tao Luo, Jia-Jun Zhou

et al.

ZooKeys, Journal Year: 2023, Volume and Issue: 1141, P. 1 - 28

Published: Jan. 17, 2023

Sinocyclocheiluslongicornussp. nov. is described from the Pearl River basin in Hongguo Town, Panzhou City, Guizhou Province, Southwest China. Based on presence of long horn-like structure back head, assigned to Sinocyclocheilusangularis species group. distinguished its congeners by a combination morphological characters: (1) single, relatively head; (2) pigmentation absent; (3) reduced eyes; (4) dorsal-fin rays, ii, 7; (5) pectoral-fin i, 13; (6) anal-fin iii, 5; (7) pelvic-fin (8) lateral line pores 38-49; (9) gill rakers well developed, nine first arch; and (10) tip adpressed pelvic fin not reaching anus.

Language: Английский

Citations

11
The complete mitochondrial genomes and phylogenetic analysis of two Chinese endemic cave fishes, Sinocyclocheilus guilinensis and S. huangtianensis (Cypriniformes: Cyprinidae) DOI Creative Commons

Guojin Yao,

You He,

Chenhong Li

et al.

Mitochondrial DNA Part B, Journal Year: 2025, Volume and Issue: 10(3), P. 167 - 172

Published: Feb. 2, 2025

Sinocyclocheilus is a group of cyprinid fishes endemic to China distributed in the karst region southwest China. In this study, complete mitogenomes guilinensis Ji, 1985 and huangtianensis Zhu et al. Citation2011 are reported characterized. Both genomes contain 13 protein-coding genes, 22 tRNAs, 2 rRNAs, non-coding control region, with lengths 16,576 bp 16,578 bp, respectively. Phylogenetic analysis shows that S. earliest branching species jii group, while sister groups. These mitochondrial valuable for studying systematics Sinocyclocheilus.

Language: Английский

Citations

0
A new species of Sinocyclocheilus (Cypriniformes, Cyprinidae) from the Beipan-Jiang in Guizhou, China DOI Creative Commons
L Q Wang, Qi Luo, Renyi Zhang

et al.

ZooKeys, Journal Year: 2025, Volume and Issue: 1238, P. 99 - 114

Published: May 13, 2025

Sinocyclocheilus zhenningensis sp. nov. from the Dabang-He of Beipan-Jiang Basin in Zhenning County, Guizhou Province, China, is described based on morphological and molecular evidence. The new species can be distinguished morphologically all congeners this genus by: (1) normal eyes (5.8%–8.2% SL), presence pigmentation, with a humpback no horn structure; (2) pelvic fin rays ii-8, dorsal-fin iii-8, stiff stout last unbranched ray; (3) body depth (26.5%–30.1% SL) covered irregular black spots, scaled, but scales buried beneath skin; (4) lateral line complete, slightly curved ventrally, 42–47 pored scales; (5) pectoral short, not reaching anterior base fin. Based mitochondrial cytochrome b ( Cytb ) gene phylogenetic tree, S. strongly supported as sister to cyphotergous - multipunctatus subclade. minimum genetic distance between its congeneric 3.8%.

Language: Английский

Citations

0
Evolution in the dark: Unexpected genetic diversity and morphological stasis in the blind, aquifer-dwelling catfish Horaglanis DOI Creative Commons
Rajeev Raghavan, Remya L. Sundar,

C. P. Arjun

et al.

Vertebrate Zoology, Journal Year: 2023, Volume and Issue: 73, P. 57 - 74

Published: Jan. 25, 2023

The lateritic aquifers of the southern Indian state Kerala harbour a unique assemblage enigmatic stygobitic fishes which are encountered very rarely, only when they surface during digging and cleaning homestead wells. Here, we focus on one most unusual members this group, catfish Horaglanis , genus rarely-collected, tiny, blind, pigment less, strictly aquifer-residing species. A six-year exploratory citizen-science backed survey supported by molecular phylogenetic analysis reveals novel insights into diversity, distribution population structure . is characterized high levels intraspecific interspecific genetic divergence, with phylogenetically distinct species recovered above 7.0% genetic-distance threshold in mitochondrial cytochrome oxidase subunit 1 gene. Contrasting deep however, remarkable stasis external morphology. We identify describe new cryptic species, populi lineage that sister group all currently known All four represented multiple haplotypes. Mismatch populations have not experienced recent expansions.

Language: Английский

Citations

9
Description of a new troglobitic Sinocyclocheilus (Pisces, Cyprinidae) species from the upper Yangtze River Basin in Guizhou, South China DOI Creative Commons
Wei‐Han Shao,

Guang-Yuan Cheng,

Xiaolong Lu

et al.

Zoosystematics and Evolution, Journal Year: 2024, Volume and Issue: 100(2), P. 515 - 529

Published: May 14, 2024

Sinocyclocheilus guiyang , a new troglobitic species from subterranean tributary of the upper Yangtze Basin in Guiyang City, Guizhou Province, China is described present study. The distinguishable its congeneric by combination following characters: tip maxillary barbel reaching to posterior edge orbit; forehead horn absent; eye absent (or highly reduced) and pectoral fins not significantly extending beyond base pelvic fin. Molecular evidence, based on mitochondrial cytochrome b (cyt ) gene, further supports validity also reveals close relationship with S. cyphotergous multipunctatus punctatus sanxiaensis. In addition, faces high risk extinction, underscoring urgency for habitat protection measures within limited range.

Language: Английский

Citations

3
A new cavefish of Sinocyclocheilus (Teleostei: Cypriniformes: Cyprinidae) from the Nanpanjiang River in Guizhou, China DOI
Qi Luo, Qian Tang, Lei Deng

et al.

Journal of Fish Biology, Journal Year: 2023, Volume and Issue: 104(2), P. 484 - 496

Published: June 24, 2023

Abstract A new species, Sinocyclocheilus xingyiensis , is described based on specimens collected from a karst cave in Guizhou Province, China. The authors used an integrated taxonomic approach, including morphological and molecular data, to identify the species as member of Sinocyclocheilu angularis group, it can be distinguished all other members this group by combination following features: two pairs long barbels pectoral fins, 42–46 lateral‐line scales, 7 (13–14) outer (inner) side first gill arch 35 (14–15 + 4 16 − 17) vertebrae. Phylogenetic analyses cytochrome b (cyt ) gene fragment suggest that S. sister lineage flexuosdorsalis. genetic distance (Kimura 2‐parameter) between groups cyt ranged 1.2% 15.4%.

Language: Английский

Citations

7
Sensory evolution in a cavefish radiation: patterns of neuromast distribution and associated behaviour inSinocyclocheilus(Cypriniformes: Cyprinidae) DOI
陈兵 Chen Bing, Tingru Mao, Yewei Liu

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1984)

Published: Oct. 12, 2022

The genus Sinocyclocheilus, comprising a large radiation of freshwater cavefishes, are well known for their presence regressive features (e.g. variable eye reduction). Fewer constructive known, such as the expansion lateral line system (LLS), which is involved in detecting water movements. precise relationship between LLS and cave adaptation not understood. Here, we examine morphology LLS-mediated behaviour Sinocyclocheilus species characterized by broad variation size, habitat geographical distribution. Using live-staining techniques automated behavioural analyses, examined 26 quantified neuromast organ number, density asymmetry within phylogenetic context. We then how these morphological may relate to wall-following, an established cave-associated mediated line. show that most demonstrated laterality (i.e. asymmetry) organs on head, often biased right. also found wall-following was distinctive, particularly among eyeless species. Patterns appear correlate with degree loss, This work reveals evolution convergent across distant cavefish taxa mediate asymmetric enable survival stark subterranean microenvironments.

Language: Английский

Citations

11
Sinocyclocheilus xiejiahuai (Cypriniformes, Cyprinidae), a new cave fish with extremely small population size from western Guizhou, China DOI Creative Commons
Fan Cui, Man Wang,

JiaJia Wang

et al.

ZooKeys, Journal Year: 2024, Volume and Issue: 1214, P. 119 - 141

Published: Oct. 3, 2024

This study describes a new species, Sinocyclocheilus xiejiahuai sp. nov ., discovered within cave located in Hongguo Town, Panzhou City, Guizhou Province, southwestern China, with the type locality Nanpanjiang River basin. Phylogenetic trees reconstructed based on mitochondrial genes show that species represents an independent evolutionary lineage large genetic differences, 1.9%–13.8% Cyt b , from congeners. Morphologically, this can be differentiated 79 currently classified under genus by several characteristics: absence of horn-like structures and indistinct elevation at head-dorsal junction, irregular black markings body lateral scaleless, eyes large, eye diameter 13% head length, dorsal-fin rays, iii, 6½, last unbranched ray strong, serrations along posterior margin, pectoral-fin i, 13, anal-fin 5, pelvic-fin 7, line pores 74, gill rakers well developed, nine first arch, pectoral fins short, tip not reaching to origin. The number has been increased 80 since description species.

Language: Английский

Citations

2
Protection and exploration of the scientific potential of Chinese cavefish DOI Open Access
Li Ma,

Yang Jun-xing,

Fa-Kai Lei

et al.

动物学研究, Journal Year: 2023, Volume and Issue: 44(4), P. 675 - 677

Published: Jan. 1, 2023

Language: Английский

Citations

5