Sex-specific role of RNA-binding protein, pAUF1, on prolonged hypersensitivity after repetitive ischemia with reperfusion injury DOI

Meranda M. Quijas,

Luis F. Queme,

Samantha T. Woodke

et al.

Pain, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Repetitive ischemia with reperfusion (I/R) injury is a common cause of myalgia. Ischemia injuries occur in many conditions that differentially affect males and females including complex regional pain syndrome fibromyalgia. Our preclinical studies have indicated primary afferent sensitization behavioral hypersensitivity caused by I/R may be due to sex-specific gene expression the dorsal root ganglia (DRG) distinct upregulation growth factors cytokines affected muscles. To determine how these unique programs established sex-dependent manner model more closely mimics clinical scenarios, we used developed prolonged ischemic myalgia mice whereby animals experience repeated compared results unbiased targeted screening strategies male female DRG. Several proteins were found expressed DRG, phosphorylated AU-rich element RNA-binding protein (pAUF1), which known regulate expression. Nerve-specific siRNA-mediated knockdown AUF1 inhibited only, whereas overexpression DRG neurons increased pain-like responses. was able specifically inhibit I/R-induced potentially downstream prolactin receptor signaling. Data suggest such as pAUF1 underlie effects on modulates after through This study aid finding differences related evolution acute chronic muscle development between sexes.

Language: Английский

Pain-sensorimotor interactions: New perspectives and a new model DOI Creative Commons
Greg M. Murray, Barry J. Sessle

Neurobiology of Pain, Journal Year: 2024, Volume and Issue: 15, P. 100150 - 100150

Published: Jan. 1, 2024

Language: Английский

Citations

5
Transcriptional profiles of non-neuronal and immune cells in mouse trigeminal ganglia DOI Creative Commons

Jennifer Mecklenburg,

Sergey A. Shein,

Mostafa Malmir

et al.

Frontiers in Pain Research, Journal Year: 2023, Volume and Issue: 4

Published: Oct. 31, 2023

Non-neuronal cells constitute 90%-95% of sensory ganglia. These cells, especially glial and immune play critical roles in the modulation neurons. This study aimed to identify, profile, summarize types trigeminal ganglion (TG) non-neuronal naïve male mice using published our own data generated by single-cell RNA sequencing, flow cytometry, immunohistochemistry. TG has five namely, glial, fibroblasts, smooth muscle, endothelial, cells. There is an agreement among publications for endothelial Based on gene profiles, were classified as myelinated non-myelinated Schwann satellite Mpz dominant expression Fabp7 specific SCG. Two Col1a2+ fibroblasts located throughout distinguished. muscle blood vessels detected well-defined markers. Our reported three macrophages (Mph) four neutrophils (Neu) TG. Mph neuronal bodies nerve fibers sub-grouped unique transcriptomic profiles with Ccr2, Cx3cr1, Iba1 A comparison databases showed that type 1 similar choroid plexus-low (CPlo) border-associated (BAMs). Type 2 highest prediction score CPhi BAMs, while 3 distinct. S100a8+ Neu dura surrounding clustering expressions Csf3r, Ly6G, Ngp, Elane, Mpo. Integrative analysis datasets indicated Neu-1, Neu-2, Neu-3 are brain Neu-1 group, Neu-4 a resemblance monocyte-derived Overall, summarized composition myeloid cell could provide essential fundamental information studies plasticity, interactomic networks between neurons function during variety pain conditions head neck regions.

Language: Английский

Citations

12
Sports and Immunity, from the recreational to the elite athlete DOI Creative Commons
Richard Baskerville,

L M Castell,

Stéphane Bermon

et al.

Infectious Diseases Now, Journal Year: 2024, Volume and Issue: 54(4), P. 104893 - 104893

Published: March 24, 2024

The pivotal role of the immune system in physical activity is well-established. While interactions are complex, they tend to constitute discrete responses. Moderate intensity exercise causes leukocytosis with a mild anti-inflammatory cytokine profile and immunoenhancement. Above threshold intensity, lactate-mediated IL-6 release proinflammatory state followed by depressed inflammatory state, which stimulates adaptation longer term cardiometabolic enhancement. Exercise-related responses modulated sex, age immunonutrition. At all ability levels, these factors collectively affect balance between enhancement or overload dysfunction. Excessive training, mental stress insufficient recovery risks cell exhaustion hypothalamic pituitary axis (HPA) causing immunodepression negative impacts on performance general health. Participation sport provides additional benefits terms ensuring regularity, social inclusion, well-being healthier life choices diet reduced smoking alcohol, thereby consolidating healthy lifestyles Significant differences exist recreational professional athletes inherent characteristics, training resilience stresses arising from competition schedules, travel-related infections stress. Exercise immunology examines central immunity physiology straddles multiple disciplines ranging neuroendocrinology nutrition genetics, aim guiding train optimally safely. This review brief outline main exercise, some influencing factors, current guidance maintaining

Language: Английский

Citations

4
The influence of sex on activity in voluntary wheel running, forced treadmill running, and open field testing in mice DOI Creative Commons
Adam J. Janowski, Giovanni Berardi, Kazuhiro Hayashi

et al.

Physiological Reports, Journal Year: 2025, Volume and Issue: 13(4)

Published: Feb. 1, 2025

Abstract Physical activity is commonly used for both measuring and treating dysfunction. While preclinical work has been historically biased towards males, the inclusion of males females gaining popularity. With increasing sexes, it imperative to determine sex differences in common behavioral assays. This was a secondary analysis healthy naïve mice baseline three assays: voluntary wheel running (32 mice), forced treadmill (178 open field (88 mice). In running, showed greater distance run, time, bout duration, speed, but no difference total bouts. time exhaustion, maximum speed attained. field, active over 30 min; however, male downward trajectory final 20 min testing, whereas did not. These data suggest that demonstrate comparable intensity female do not match females' duration activity, especially volitional tasks. Researchers utilizing these assays should account as they could mask true findings an experiment.

Language: Английский

Citations

0
The Role of Exercise on Glial Cell Activity in Neuropathic Pain Management DOI Creative Commons
Willians Fernando Vieira, Caroline Cristiano Real, Daniel Oliveira Martins

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 487 - 487

Published: March 24, 2025

Chronic pain is a widespread global health problem with profound socioeconomic implications, affecting millions of people all ages. Glial cells (GCs) in pathways play essential roles the processing signals. Dysregulation GC activity contributes to chronic states, making them targets for therapeutic interventions. Non-pharmacological approaches, such as exercise, are strongly recommended effective management. This review examines link between regular physical (PA), and glial cell-mediated processing, highlighting its potential strategy managing pain. Exercise not only improves overall quality life but also influences function GCs. Recent research highlights ability exercise mitigate neuroinflammatory responses modulate GCs by reducing activation microglia astrocytes, well modulating expression biomarkers, thereby attenuating hypersensitivity. Here, we summarize new insights into role non-pharmacological intervention relief

Language: Английский

Citations

0
Advances in Gouty Arthritis Management: Integration of Established Therapies, Emerging Treatments, and Lifestyle Interventions DOI Open Access

Ting-Kuo Yao,

Ru‐Ping Lee, Wen‐Tien Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10853 - 10853

Published: Oct. 9, 2024

Gouty arthritis, a prevalent inflammatory condition characterized by the deposition of monosodium urate crystals within joints, often results in debilitating pain and inflammation. Conventional therapeutic approaches, including nonsteroidal anti-inflammatory drugs, corticosteroids, urate-lowering agents such as allopurinol febuxostat, have limitations adverse effects, drug interactions, suboptimal patient compliance. This review presents comprehensive overview both established emerging strategies, developed between 2019 2024, for gouty arthritis; focuses on their mechanisms action, efficacy, safety profiles. Novel approaches include pharmaceutical plant additives (e.g., Citrullus colocynthis, Atractylodes lancea), canakinumab ozone therapy, complementary therapies warm ginger compresses, Qingpeng ointment, various lifestyle modifications. These strategies offer promising alternatives to conventional treatments targeting uric acid metabolism, pathways, crystal formation, potentially reducing reliance standard medications minimizing effects. Although demonstrated significant efficacy gout flares, others polyphenol-rich foods favorable Further research, large-scale clinical trials, is warranted validate these findings integrate into practice improve outcomes quality life.

Language: Английский

Citations

3
Brain-derived neurotrophic factor contributes to activity-induced muscle pain in male but not female mice DOI
Kazuhiro Hayashi, Joseph B. Lesnak, Ashley N. Plumb

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 120, P. 471 - 487

Published: June 24, 2024

Language: Английский

Citations

2
The Role of Exercise in the Alleviation of Neuropathic Pain Following Traumatic Spinal Cord Injuries: A Systematic Review and Meta-analysis DOI Creative Commons
Amirmohammad Toloui, Hamzah Adel Ramawad, Pantea Gharin

et al.

Neurospine, Journal Year: 2023, Volume and Issue: 20(3), P. 1073 - 1087

Published: Sept. 22, 2023

The objective of this systematic review and meta-analysis was to assess the efficacy exercise in neuropathic pain following traumatic spinal cord injuries.The search conducted MEDLINE, Embase, Scopus, Web Science by end 2022. Two independent researchers included articles based on inclusion exclusion criteria. A standardized mean difference calculated for each data they were pooled calculate an overall effect size. To heterogeneity between studies, I2 chi-square tests utilized. In case heterogeneity, meta-regression performed identify potential source.Fifteen preclinical studies included. Meta-analysis demonstrated that significantly improves mechanical allodynia (standardized [SMD], -1.59; 95% confidence interval [CI], -2.16 -1.02; p < 0.001; = 90.37%), thermal hyperalgesia (SMD, 1.95; CI, 0.96-2.94; 0.001), cold -2.92; -4.4 -1.43; 0.001). improvement is more animals with a compression model SCI (meta-regression coefficient, -1.33; -1.84 -0.57; 0.001) mild (p Additionally, prominent if training started 7 8 days postinjury (coefficient, -2.54; -3.85 -1.23; continued every day -1.99; -3.07 -0.9; Likewise, voluntary size -1.45; -2.67 -0.23; 0.02).Exercise effective amelioration pain. This voluntary, continuous initiated subacute phase SCI.

Language: Английский

Citations

6
Transcriptional Profiles of Non-neuronal and Immune Cells in Mouse Trigeminal Ganglia DOI Open Access

Jennifer Mecklenburg,

Sergey A. Shein,

Anahit H. Hovhannisyan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 19, 2023

Non-neuronal cells constitute 90-95% of sensory ganglia. These play critical roles in modulation nociceptive signal transmissions by neurons. Accordingly, the aim this review-study was to identify, profile and summarize TG non-neuronal cell types naïve male mice using published our own data generated single-cell RNA sequencing (scRNA-seq), flow cytometry (FC) immunohistochemistry (IHC). contains 5 cells: glial, fibroblasts, smooth muscle, endothelial immune cells. There is agreement among publications for muscle Based on gene profiles, glial were classified as Schwann satellite (SGC). Mpz had dominant expression cells, Fabp7 specific SCG. Two Col1a2 + fibroblasts located throughout distinguished profiles. representing blood vessels detected with well recognized markers. Our study split reported single group into 3 macrophages 4 neutrophils. Macrophages neuronal bodies nerve fibers, sub-grouped unique transcriptomic profiles Ccr2 , Cx3cr1 Iba1 S100a8 neutrophils dura surrounding clustering expressions Csf3r Ly6G, Ngp, Elane Mpo . Overall, summarized here dataset could provide essential fundamental information studies plasticity, interactomic network between neurons function during variety pain conditions head neck region.

Language: Английский

Citations

2
Brain-derived neurotrophic factor contributes to activity-induced muscle pain in male but not female mice DOI Creative Commons
Kazuhiro Hayashi, Joseph B. Lesnak, Ashley N. Plumb

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 2, 2023

Abstract Activity-induced muscle pain increases release of interleukin-1β (IL-1β) in macrophages and the development is prevented by blockade IL-1β. Brain derived neurotrophic factor (BDNF) released from sensory neurons response to IL-1β mediates both inflammatory neuropathic pain. Thus, we hypothesized that metabolites during fatiguing contractions activate IL-1β, which subsequently secrete BDNF. To test this hypothesis, used an animal model activity-induced induced repeated intramuscular acidic saline injections combined with contractions. Intrathecal or injection inhibitors BDNF-Tropomyosin receptor kinase B (TrkB) signaling, ANA-12 TrkB-Fc, reduced decrease withdrawal thresholds male, but not female, mice when given before 24hr after, 1 week after induction model. BDNF messenger ribonucleic acid (mRNA) was significantly increased L4–L6 dorsal root ganglion (DRG), spinal horn gastrocnemius muscle, either male female mice. No changes TrkB mRNA p75 neurotrophin were observed. protein expression via immunohistochemistry retrogradely labelled afferent DRG neurons, at sexes. In cultured DRG, fatigue summary, release, primary contribute only males, while there no sex differences observed

Language: Английский

Citations

2