Sex-specific role of RNA-binding protein, pAUF1, on prolonged hypersensitivity after repetitive ischemia with reperfusion injury DOI

Meranda M. Quijas,

Luis F. Queme,

Samantha T. Woodke

et al.

Pain, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Repetitive ischemia with reperfusion (I/R) injury is a common cause of myalgia. Ischemia injuries occur in many conditions that differentially affect males and females including complex regional pain syndrome fibromyalgia. Our preclinical studies have indicated primary afferent sensitization behavioral hypersensitivity caused by I/R may be due to sex-specific gene expression the dorsal root ganglia (DRG) distinct upregulation growth factors cytokines affected muscles. To determine how these unique programs established sex-dependent manner model more closely mimics clinical scenarios, we used developed prolonged ischemic myalgia mice whereby animals experience repeated compared results unbiased targeted screening strategies male female DRG. Several proteins were found expressed DRG, phosphorylated AU-rich element RNA-binding protein (pAUF1), which known regulate expression. Nerve-specific siRNA-mediated knockdown AUF1 inhibited only, whereas overexpression DRG neurons increased pain-like responses. was able specifically inhibit I/R-induced potentially downstream prolactin receptor signaling. Data suggest such as pAUF1 underlie effects on modulates after through This study aid finding differences related evolution acute chronic muscle development between sexes.

Language: Английский

Sex specific role of RNA-binding protein, AUF1, on prolonged hypersensitivity after repetitive ischemia with reperfusion injury DOI Creative Commons

Meranda M. Quijas,

Luis F. Queme,

Alex A. Weyler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 9, 2023

Repetitive ischemia with reperfusion (I/R) injury is a common cause of myalgia. I/R injuries occur in many conditions that differentially affect males and females including complex regional pain syndrome fibromyalgia. Our preclinical studies have indicated primary afferent sensitization behavioral hypersensitivity due to may be sex specific gene expression the DRGs distinct upregulation growth factors cytokines affected muscles. In order determine how these unique programs established dependent manner model more closely mimics clinical scenarios, we utilized newly developed prolonged ischemic myalgia mice whereby animals experience repeated forelimb compared results unbiased targeted screening strategies male female DRGs. Several proteins were found expressed DRGs, AU-rich element RNA binding protein (AUF1), which known regulate expression. Nerve siRNA-mediated knockdown AUF1 inhibited only, while overexpression DRG neurons increased some pain-like responses. Further, was able specifically inhibit induced but not males. Data suggests like underlie effects on modulate after injury. This study aid finding receptor differences related evolution acute chronic muscle development between sexes.

Language: Английский

Citations

1
Is exercise therapy the first-line treatment for chronic pain? DOI Creative Commons

Emiko Senba

Neurobiology of Pain, Journal Year: 2024, Volume and Issue: 15, P. 100154 - 100154

Published: Jan. 1, 2024

Language: Английский

Citations

0
The influence of sex on activity in voluntary wheel running, forced treadmill running, and open field testing DOI Creative Commons
Adam J. Janowski, Giovanni Berardi, Kazuhiro Hayashi

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 14, 2024

Physical activity is commonly used for both measuring and treating dysfunction. While preclinical work has been historically biased towards males, the use of male female animals gaining popularity after multiple NIH initiatives. With increasing inclusion sexes, it become imperative to determine sex differences in common behavioral assays. The purpose this study was baseline 3 assays: voluntary wheel running, forced treadmill open field testing.

Language: Английский

Citations

0
Future research and applications DOI
Jiří Gallo, Stuart B. Goodman, Michiaki Takagi

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 169 - 182

Published: Jan. 1, 2024

Language: Английский

Citations

0
Sex-specific role of RNA-binding protein, pAUF1, on prolonged hypersensitivity after repetitive ischemia with reperfusion injury DOI

Meranda M. Quijas,

Luis F. Queme,

Samantha T. Woodke

et al.

Pain, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Repetitive ischemia with reperfusion (I/R) injury is a common cause of myalgia. Ischemia injuries occur in many conditions that differentially affect males and females including complex regional pain syndrome fibromyalgia. Our preclinical studies have indicated primary afferent sensitization behavioral hypersensitivity caused by I/R may be due to sex-specific gene expression the dorsal root ganglia (DRG) distinct upregulation growth factors cytokines affected muscles. To determine how these unique programs established sex-dependent manner model more closely mimics clinical scenarios, we used developed prolonged ischemic myalgia mice whereby animals experience repeated compared results unbiased targeted screening strategies male female DRG. Several proteins were found expressed DRG, phosphorylated AU-rich element RNA-binding protein (pAUF1), which known regulate expression. Nerve-specific siRNA-mediated knockdown AUF1 inhibited only, whereas overexpression DRG neurons increased pain-like responses. was able specifically inhibit I/R-induced potentially downstream prolactin receptor signaling. Data suggest such as pAUF1 underlie effects on modulates after through This study aid finding differences related evolution acute chronic muscle development between sexes.

Language: Английский

Citations

0