Review: Citizen science: How to extend reciprocal benefits from the project community to the broader socio-ecological system — R1/PR7 DOI Creative Commons
Aurore Receveur, Lucie Poulet, Benjamin Dalmas

et al.

Published: March 22, 2022

Quantitative plant biology is a growing field, thanks to the substantial progress of models and artificial intelligence dealing with big data. However, collecting large enough datasets not always straightforward. The citizen science approach can multiply workforce, hence helping researchers data collection analysis, while also facilitating spread scientific knowledge methods volunteers. reciprocal benefits go far beyond project community: By empowering volunteers increasing robustness results, method spreads socio-ecological scale. This review aims demonstrate that has huge potential (i) for development different tools collect analyse much larger datasets, (ii) by their involvement in governance (iii) system share knowledge, cascade effect help ‘facilitators’.

Language: Английский

'Qualitative' and 'quantitative' methods and approaches across subject fields: implications for research values, assumptions, and practices DOI Creative Commons
Nick Pilcher,

Martin Cortazzi

Quality & Quantity, Journal Year: 2023, Volume and Issue: 58(3), P. 2357 - 2387

Published: Sept. 30, 2023

Abstract There is considerable literature showing the complexity, connectivity and blurring of 'qualitative' 'quantitative' methods in research. Yet these concepts are often represented a binary way as independent dichotomous categories. This evident many key textbooks which used research courses to guide students newer researchers their training. paper analyses such textbook representations 25 resources published English (supported by an outline survey 23 written German, Spanish French). We then compare with perceptions, gathered through semi-structured interviews, university (n = 31) who work wide range arts science disciplines. The analysis what say compared participants report they do practice shows some common features, might be assumed, but there significant contrasts contradictions. differences tend align other recent underline complexity associated terms. suggest ways future could question positively deconstruct order free up directions for practice, so that investigations can use both quantitative or qualitative approaches more nuanced practices appropriate specific field given context investigations.

Language: Английский

Citations

28
Imaging the living plant cell: From probes to quantification DOI Creative Commons
Léia Colin, Raquel Martin‐Arevalillo, Simone Bovio

et al.

The Plant Cell, Journal Year: 2021, Volume and Issue: 34(1), P. 247 - 272

Published: Sept. 23, 2021

At the center of cell biology is our ability to image and its various components, either in isolation or within an organism. Given importance, biological imaging has emerged as a field own, which inherently highly interdisciplinary. Indeed, biologists rely on physicists engineers build new microscopes techniques, chemists develop better probes, mathematicians computer scientists for analysis quantification. Live collectively involves all techniques aimed at live samples. It rapidly evolving field, with countless dyes being continuously developed. Some these methods reagents are readily amenable plant samples, while others not require specific modifications field. Here, we review some recent advances cells. In particular, discuss solutions that use membrane-bound organelles, cytoskeleton hormones, mechanical properties cells tissues. We only consider per se, but also how construction fluorescent probes pipelines driving biology.

Language: Английский

Citations

41
Citizen science: How to extend reciprocal benefits from the project community to the broader socio-ecological system DOI Creative Commons
Aurore Receveur, Lucie Poulet, Benjamin Dalmas

et al.

Quantitative Plant Biology, Journal Year: 2022, Volume and Issue: 3

Published: Jan. 1, 2022

Abstract Quantitative plant biology is a growing field, thanks to the substantial progress of models and artificial intelligence dealing with big data. However, collecting large enough datasets not always straightforward. The citizen science approach can multiply workforce, hence helping researchers data collection analysis, while also facilitating spread scientific knowledge methods volunteers. reciprocal benefits go far beyond project community: By empowering volunteers increasing robustness results, method spreads socio-ecological scale. This review aims demonstrate that has huge potential (i) for development different tools collect analyse much larger datasets, (ii) by their involvement in governance (iii) system share knowledge, cascade effect help ‘facilitators’.

Language: Английский

Citations

25
Looking beyond the gene network – metabolic and mechanical cell drivers of leaf morphogenesis DOI Creative Commons
Hokuto Nakayama, Hiroyuki Koga, Yuchen Long

et al.

Journal of Cell Science, Journal Year: 2022, Volume and Issue: 135(8)

Published: April 15, 2022

ABSTRACT The above-ground organs in plants display a rich diversity, yet they grow to characteristic sizes and shapes. Organ morphogenesis progresses through sequence of key events, which are robustly executed spatiotemporally as an emerging property intrinsic molecular networks while adapting various environmental cues. This Review focuses on the multiscale control leaf morphogenesis. Beyond list known genetic determinants underlying growth shape, we focus instead novel mechanisms metabolic biomechanical regulations that coordinate plant cell non-cell-autonomously. reveals how metabolism mechanics not solely passive outcomes regulation but play instructive roles Such integrative view also extends fluctuating cues evolutionary adaptation. synthesis calls for more balanced morphogenesis, where shapes considered from standpoints geometry, genetics, energy mechanics, properties cellular expression these different properties.

Language: Английский

Citations

24
Skotomorphogenesis exploits threonine to promote hypocotyl elongation DOI Creative Commons
Hiromitsu Tabeta, Yasuhiro Higashi, Yozo Okazaki

et al.

Quantitative Plant Biology, Journal Year: 2022, Volume and Issue: 3

Published: Jan. 1, 2022

Mobilisation of seed storage reserves is important for seedling establishment in

Language: Английский

Citations

18
An auxin homeostat allows plant cells to establish and control defined transmembrane auxin gradients DOI
Markus Geisler, Ingo Drèyer

New Phytologist, Journal Year: 2024, Volume and Issue: 244(4), P. 1422 - 1436

Published: Sept. 15, 2024

Summary Extracellular auxin maxima and minima are important to control plant developmental programs. Auxin gradients provided by the concerted action of proteins from three major plasma membrane (PM) transporter classes AUX1/LAX, PIN ATP‐BINDING CASSETTE subfamily B (ABCB) transporters. But neither genetic nor biochemical modeling approaches have been able reliably assign individual roles interplay these types. Based on thermodynamic properties transporters, we show here mathematical computational simulations that different types allows adjustment specific transmembrane gradients. The dynamic flexibility ‘auxin homeostat’ comes at cost an energy‐consuming cycling’ across membrane. An unexpected finding was potential functional ABCB‐PIN synchronization appears allow optimization trade‐off between speed PM gradient one hand ATP consumption disturbance general anion homeostasis other. In conclusion, our analyses provide fundamental insights into constraints transport in plants.

Language: Английский

Citations

3
The 1972 Meadows report: A wake-up call for plant science DOI Creative Commons
Olivier Hamant

Quantitative Plant Biology, Journal Year: 2023, Volume and Issue: 4

Published: Jan. 1, 2023

The 1972 Meadows report, 'the limits to growth', predicted a global socio-economic tipping point during the twenty-first century. Now supported by 50 years of empirical evidence, this work is tribute systems thinking and an invitation take current environmental crisis for what it is: neither transition nor bifurcation, but inversion. For instance, we used matter (e.g., fossil fuel) save time; will use time preserve bioeconomy). We were exploiting ecosystems fuel production; production feed ecosystems. centralised optimise; decentralise support resilience. In plant science, new context calls research on complexity multiscale robustness benefits variability), also extending scientific approaches participatory research, art science). Taking turn reverses many paradigms becomes responsibility scientists as world increasingly turbulent.

Language: Английский

Citations

8
A quantitative model for spatio-temporal dynamics of root gravitropism DOI Creative Commons
Amir Porat, M Rivière, Yasmine Meroz

et al.

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(2), P. 620 - 630

Published: Oct. 23, 2023

Plant organs adapt their morphology according to environmental signals through growth-driven processes called tropisms. While much effort has been directed towards the development of mathematical models describing tropic dynamics aerial organs, these cannot provide a good description roots due intrinsic physiological differences. Here we present model informed by gravitropic experiments on Arabidopsis thaliana roots, assuming subapical growth profile and apical sensing. The quantitatively recovers full spatio-temporal observed in experiments. An analytical solution enables us evaluate proprioceptive sensitivities while also allowing corroborate requirement for proprioception root dynamics. Lastly, find that are analogous damped harmonic oscillator, providing intuition regarding source oscillatory behavior importance efficient control. In all, provides not only quantitative dynamics, but framework future investigation complex media.

Language: Английский

Citations

8
An auxin homeostat allows plant cells to establish and control defined transmembrane auxin gradients DOI Creative Commons
Markus Geisler, Ingo Drèyer

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Summary Extracellular auxin maxima and minima are important to control plant developmental programs. Auxin gradients provided by the concerted action of proteins from three major plasma membrane transporter classes AUX1/LAX, PIN ABCB transporters. But neither genetic nor biochemical modelling approaches have been able reliably assign individual roles interplay these types. Based on thermodynamic properties transporters, we show here mathematical modeling computational simulations that different types allow adjustment specific transmembrane gradients. The dynamic flexibility “auxin homeostats” comes at cost an energy-consuming cycling” across membrane. An unexpected finding was functional ABCB-PIN coupling appears optimization trade-off between speed gradient one hand ATP consumption disturbance general anion homeostasis other. In conclusion, our analyses provide fundamental insights into constraints transport in plants. Plain language summary phytohormone controls essentially development. Plant cells produce export it establish patterns local maxima. Although several known contribute this process, mechanism which a defined can be produced is not clear. This study now uses based features transporters illustrate characteristics homeostat”. allows indispensable basis for polarized fluxes within tissues.

Language: Английский

Citations

2
Leaf-size control beyond transcription factors: Compensatory mechanisms DOI Creative Commons
Hiromitsu Tabeta,

Shizuka Gunji,

Kensuke Kawade

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 23, 2023

Plant leaves display abundant morphological richness yet grow to characteristic sizes and shapes. Beginning with a small number of undifferentiated founder cells, evolve

Language: Английский

Citations

6