Microchimica Acta, Journal Year: 2021, Volume and Issue: 188(4)
Published: March 4, 2021
Language: Английский
Journal of Applied Physics, Journal Year: 2021, Volume and Issue: 129(19)
Published: May 17, 2021
Surface-enhanced Raman scattering (SERS) technique has shown extraordinary features for biomedical applications. The implementation of SERS nanotags opened a new era bioimaging and detections. As powerful tool, provide favorable properties such as fingerprint spectrum, narrow peak linewidth, good photostability, high spatial resolution accompanied by various rational designs nanoparticles. They have proven useful imaging agents in vivo, ex vitro detection cancerous cells tissues. This tutorial provides the basic principles nanotags, including recent progress SERS-based applications, well outlooks into future developments toward practical clinical SERS.
Language: Английский
Citations
65
Analytical Chemistry, Journal Year: 2020, Volume and Issue: 92(17), P. 11755 - 11762
Published: Aug. 11, 2020
Molecular diagnosis and accurate damage analysis of complex genomic DNAs in tumor cells are crucial to the theranostics cancers but still a huge challenge. Herein, by designed preparation uniform plasmonic sunflower-like assembly gold (Au) nanostructure that is capable efficient DNA capture providing high-density gap-plasmon "hot spots" for adequate surface-enhanced Raman spectroscopy (SERS) enhancement, we succeeded sensitive reliable label-free SERS detection electrostimulus-induced apoptotic cancer at base level first time. The results showed external electrostimulus (at 1.2 V, 5 min) was almost harmless normal healthy cells, it caused pronounced double strand break adenine cell DNAs, which effectively destroyed reproduction transcription ultimately induced apoptosis. developed sensing platform method promising study genetically related diseases.
Language: Английский
Citations
49
Food Chemistry, Journal Year: 2021, Volume and Issue: 358, P. 129898 - 129898
Published: April 20, 2021
Language: Английский
Citations
34
Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(4), P. 2183 - 2190
Published: Jan. 7, 2021
Monitoring externalization of phosphatidylserine (PS) and gaining insights into molecular events cell membrane damage are significant for programmed death studies. Herein, by encapsulating zeolitic imidazole frameworks-8 with plasmonic gold nanoparticles to form micron-nano composites using them as a single-particle surface-enhanced Raman spectroscopy (SERS) substrate, we succeeded in real-time discriminating monitoring the PS on membranes during electrostimulus-induced apoptosis. The composite probe provides rich "hot spots" robust anchoring capacity membranes, achieving capture label-free SERS detection externalized PS. By this method, dynamic process differences between cancerous cells normal were clearly revealed which was more serious than that cells. This method is versatile uncovering related mechanisms. work also broadens application metal–organic framework materials advanced biomedical applications.
Language: Английский
Citations
28
Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 192, P. 113539 - 113539
Published: Aug. 3, 2021
Language: Английский
Citations
28
The Analyst, Journal Year: 2020, Volume and Issue: 145(22), P. 7162 - 7185
Published: Jan. 1, 2020
This tutorial review explores how three of the most common methods for introducing nanoparticles to single cells surface-enhanced Raman scattering measurements can be adapted experiments with complex tissues.
Language: Английский
Citations
32
Asian Journal of Pharmaceutical Sciences, Journal Year: 2021, Volume and Issue: 17(1), P. 87 - 101
Published: Nov. 14, 2021
Spinal cord injury (SCI) causes Ca2+ overload, which can lead to inflammation and neuronal apoptosis. In this study, we prepared a nanovesicle derived from macrophage membrane (MVs), encapsulated sodium alginate (SA) naloxone (NAL) inhibit protect neurons by reducing the free Ca2+concentration at SCI site. Based on transmission electron microscopy (TEM) image, sample (NAL-SA-MVs) had particle size of approximately 134 ± 11 nm exhibited sustained release effect. The encapsulation rate NAL SA was 82.07% 3.27% 72.13% 2.61% in NAL-SA-MVs, respectively. Targeting tests showed that NAL-SA-MVs could accumulate large quantities enhance concentration lesion sites. vivo vitro studies indicated decrease Ca2+, should further alleviate inflammatory response Anti-inflammation results demonstrated reduce pro-inflammation factors (iNOS, TNF-α, IL-1β, IL-6) increase expression anti-inflammation (IL-10) cell animal level. Concurrently, fluorescence, flow cytometry western blot characterization apoptotic condition significantly inhibited. addition, motor function C57 mice were improved after treatment. conclusion, it is suggested has tremendous potential treatment SCI.
Language: Английский
Citations
27
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 5, 2024
Abstract Nanomedicine has shown great anticancer potential by disrupting redox homeostasis and increasing the levels of oxidative stress, but therapeutic effect is limited factors including intrinsic self‐protection mechanism tumors. Cancer cell death can be induced exploration different mechanisms, such as apoptosis, pyroptosis, necroptosis, cuproptosis, ferroptosis. The merging nanotechnology with biomedicine provided tremendous opportunities to construct death‐based nanomedicine for innovative cancer therapy. Nanocarriers are not only used targeted delivery inducers, also components induce achieve efficient tumor treatment. This review focuses on seven modalities mediated nanomaterials, ferroptosis, cuprotosis, immunogenic death, autophagy. mechanisms these described in detail, well preparation nanomaterials that them they exert their effects. Finally, this work describes future development based current knowledge related nanomaterials.
Language: Английский
Citations
4
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126177 - 126177
Published: April 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(5), P. 2087 - 2093
Published: Jan. 26, 2024
Due to its direct effect on biomolecules and cells, electrical stimulation (ES) is now widely used regulate cell proliferation, differentiation, neurostimulation even in the clinic for pain relief, treatment of nerve damage, muscle rehabilitation. Conventional ES mostly studied populations, but heterogeneity cancer cells results inability access response individual ES. Therefore, detecting extracellular pH change (ΔpHe) after at single-cell level important application tumor therapy. In this study, cellular ΔpHe periodic impulse electrostimulation (IES) was monitored situ by using a polyaniline (PANI)-modified gold microelectrode array. The PANI sensor had excellent sensitivity (53.68 mV/pH) linear correlation coefficient (R2 = 0.999) over range 5.55–7.41. showed different degrees IES with intervals potential. A shorter pulse interval higher potential could effectively enhance increase ΔpHe. At 0.5 V stimulation, increased decreasing interval. However, if long enough, 0.7 V, there no significant additional due insufficient stimulus strength. Based above conclusions, prepared arrays (MEAs) were capable stimulating single which contributed deeper
Language: Английский
Citations
3