ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(21), P. 24996 - 25003
Published: Oct. 25, 2024
Observing
changes
in
cell
morphology
and
membranes
is
the
most
direct
way
to
monitor
effect
of
drug
treatment
on
tumor
cells.
Here,
a
surface-enhanced
Raman
spectroscopy
(SERS)
approach
was
developed
directly
reveal
fingerprint
information
cancer
under
different
altered
states
response
drugs
by
using
silver
nanoparticle-based
magnetic
plasmonic
(M-AgNPs)
probe.
The
M-AgNP
probe
can
quickly
spontaneously
gather
over
membrane
within
5
min.
Then,
acquired,
enabling
us
successfully
distinguish
differences
between
normal
apoptotic
before
after
treatment,
according
their
SERS
spectral
profiles.
facilitates
identification
effects
complex
biological
scenarios.
This
membrane-specific,
molecular-level
analytical
platform
offers
advantage
immediate
application
with
no
need
for
pretreatments
easy
operate.
Label-free
analysis
helps
identify
binding
sites
specific
due
variations,
which
holds
great
promise
understanding
targets
has
wide
range
potential
applications
pharmacodynamics
studies.
MedComm,
Journal Year:
2023,
Volume and Issue:
4(1)
Published: Jan. 11, 2023
Cancer
is
a
complex
disease
associated
with
combination
of
abnormal
physiological
process
and
exhibiting
dysfunctions
in
multiple
systems.
To
provide
effective
treatment
diagnosis
for
cancer,
current
strategies
simultaneously
focus
on
various
tumor
targets.
Based
the
rapid
development
nanotechnology,
nanocarriers
have
been
shown
to
exhibit
excellent
potential
cancer
therapy.
Compared
nanoparticles
single
functions,
multifunctional
are
believed
be
more
aggressive
potent
context
targeting.
However,
not
simply
an
upgraded
version
original
function,
but
involves
sophisticated
system
proper
backbone,
optimized
modification
sites,
simple
preparation
method,
efficient
function
integration.
Despite
this,
many
well-designed
promising
therapeutic
emerged
recently.
Here,
give
detailed
understanding
analyzation
currently
developed
nanoparticles,
their
platform
structures
organic
or
inorganic
backbones
were
systemically
generalized.
We
emphasized
functionalization
strategies,
which
additional
functions
nanoparticle.
also
discussed
application
that
involved
nanoformulations
functional
crosstalk.
This
review
thus
provides
overview
construction
advances
nanoparticles.
ACS Sustainable Chemistry & Engineering,
Journal Year:
2022,
Volume and Issue:
10(26), P. 8400 - 8410
Published: June 21, 2022
Substrate
materials
possessing
high
sensitivity
and
storage
stability
are
vital
for
the
practical
analytical
applications
of
surface-enhanced
Raman
scattering
(SERS)
techniques.
In
this
work,
SERS
substrate
material
(Fe3O4@UiO-66-NH2@Au)
on
a
magnetic
metal–organic
framework
(Fe3O4@UiO-66-NH2)
decorated
with
gold
nanoparticles
(AuNPs)
was
constructed.
The
as-prepared
combined
properties
abundant
hotspots
between
AuNPs
excellent
adsorption
performance
UiO-66-NH2
trace
molecules.
Meanwhile,
presence
Fe3O4
can
be
helpful
to
separate
quickly,
generation
coffee
ring
effect
weakened
under
action
an
external
field.
results
showed
that
new
have
performance.
used
TBZ
detection;
it
exhibited
good
linearity
in
range
from
0.025
2
ppm,
limit
detection
(LOD)
could
down
6.5
ppb.
signal
intensity
stable
after
22
days
at
room
temperature.
We
constructed
sensitive,
stable,
rapid
method
based
which
also
has
great
potential
food
environmental
pollution
applications.
ACS Nano,
Journal Year:
2022,
Volume and Issue:
16(9), P. 14055 - 14065
Published: Aug. 15, 2022
The
visualization
of
protein
dimerization
on
live
cells
is
an
urgent
need
and
vital
importance
for
facile
monitoring
the
signal
transduction
during
intercellular
communication.
Herein,
a
highly
sensitive
specific
SERS
strategy
simultaneously
imaging
dual
homodimerizations
membrane
proteins
single
was
proposed
by
networking
AuNPs-based
dual-recognition
probes
(dual-RPs)
tags
via
proximity
ligation-assisted
catalytic
hairpin
assembly
(CHA).
dual-RPs
were
prepared
comodifying
hairpin-structured
ssDNAs
H1-Met
H1-TβRII
50
nm
AuNPs
two
Met
TβRII
respectively
labeling
their
corresponding
Raman
molecules
single-stranded
DNAs
H2-Met
or
H2-TβRII
15
AuNPs.
ligated
proximally
aptamers,
dimerizations
resulted
in
CHA-based
to
form
15Au-50Au
network
nanostructures
with
significantly
enhanced
effect.
visualizing
established
good
performance
(i.e.,
Met-Met
TβRII-TβRII)
confirmed.
Furthermore,
dimerization-based
signaling
pathways
between
cancer
stromal
stem
observed
SERS,
which
indicates
that
method
high-sensitivity
dimerizations-based
multiple
transductions
natural
complex
cellular
microenvironment.
Nanoscale,
Journal Year:
2022,
Volume and Issue:
14(14), P. 5314 - 5328
Published: Jan. 1, 2022
Surface
enhanced
Raman
scattering
(SERS)
from
biomolecules
in
living
cells
enables
the
sensitive,
but
also
very
selective,
probing
of
their
biochemical
composition.
This
minireview
discusses
developments
SERS
over
past
years
proof-of-principle
to
observe
a
status
characterization
molecule-nanostructure
and
molecule-molecule
interactions
cellular
processes
that
involve
wide
variety
compartments.
Progress
applying
as
bioanalytical
tool
cells,
gain
better
understanding
physiology
harness
selectivity
SERS,
has
been
achieved
by
combination
live
cell
with
several
different
approaches.
They
range
organelle
targeting,
spectroscopy
relevant
molecular
models,
optimization
plasmonic
nanostructures
application
machine
learning
help
us
unify
information
defined
an
extremely
complex
system.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(2), P. 676 - 684
Published: Jan. 3, 2024
Identification
of
the
phosphatidylserine
(PS)
discrepancies
occurring
on
cellular
membrane
during
apoptotic
processes
is
utmost
importance.
However,
monitoring
quantity
PS
molecules
in
real-time
at
a
single-cell
level
currently
remains
challenging
task.
Here,
we
demonstrate
this
objective
by
leveraging
specific
binding
and
reversible
interaction
exhibited
zinc(II)
dipyridinamine
complex
(ZnDPA)
with
PS.
Lipoic
acid-functionalized
ZnDPA
(LP-ZnDPA)
was
subsequently
immobilized
onto
surface
an
atomic
force
microscopy
cantilever
to
form
probe,
ALP-ZnDPA,
enabling
PS-specific
dynamic
imaging
detection
mode.
By
utilizing
technique,
can
not
only
create
heat
map
expression
submicron
resolution
but
also
quantify
number
present
single
cell's
limit
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