Small,
Journal Year:
2023,
Volume and Issue:
20(7)
Published: Oct. 3, 2023
Abstract
Previous
on‐chip
technologies
for
characterizing
the
cellular
mechanical
properties
often
suffer
from
a
low
throughput
and
limited
sensitivity.
Herein,
an
inertial
multi‐force
deformability
cytometry
(IMFDC)
is
developed
high‐throughput,
high‐accuracy,
high‐applicability
tumor
cell
mechanotyping.
Three
different
deformations,
including
shear
deformations
stretch
under
forces,
are
integrated
with
IMFDC.
The
3D
focusing
of
cells
enables
to
deform
by
identical
fluid
flow,
10
parameters,
such
as
area,
perimeter,
deformability,
roundness,
rectangle
obtained
in
three
deformations.
IMFDC
able
evaluate
that
sensitive
forces
on
single
chip,
demonstrating
high
applicability
analyzing
lines.
In
identifying
types,
exhibit
responses
sizes
deformability.
A
discrimination
accuracy
≈93%
both
MDA‐MB‐231
MCF‐10A
≈500
s
−1
can
be
achieved
using
multiple‐parameters‐based
machine
learning
model.
Finally,
metastatic
pleural
peritoneal
effusions
characterized,
enabling
practical
application
clinical
cancer
diagnosis.
Lab on a Chip,
Journal Year:
2024,
Volume and Issue:
24(12), P. 3036 - 3063
Published: Jan. 1, 2024
Current
approaches
for
mechanical
measurements
of
single
cells
compromise
between
fidelity
and
throughput.
Development
non-contact
technologies
optimized
theoretical
modelling
will
advance
characterisation
large
cell
populations.
Lab on a Chip,
Journal Year:
2023,
Volume and Issue:
23(11), P. 2531 - 2539
Published: Jan. 1, 2023
As
a
label-free
and
high-throughput
single
cell
analysis
platform,
impedance
flow
cytometry
(IFC)
suffers
from
clogging
caused
by
narrow
microchannel
as
mechanical
constriction
(MC).
Current
sheath
(SC)
solutions
lack
systematic
evaluation
of
the
performance
proper
guidelines
for
fluid.
Herein,
we
hypothesize
that
viscosity
non-conductive
liquid
is
key
to
SC,
propose
employ
viscous
in
SC
unlock
tradeoff
between
sensitivity
throughput,
while
ensuring
measurement
accuracy.
By
placing
MC
series
same
microfluidic
chip,
established
an
platform
prove
hypothesis.
Through
modeling
experiments,
confirmed
accuracy
(error
<
1.60%
±
4.71%)
w.r.t.
MC,
demonstrated
PEG
solution
achieved
improved
(7.92×)
signal-to-noise
ratio
(1.42×)
measurement,
with
maintained
free
clogging.
Viscous
IFC
also
shows
satisfactory
ability
distinguish
different
types
cancer
cells
subtypes
human
breast
cells.
It
envisioned
paves
way
be
really
usable
practice
clogging-free,
accurate,
sensitive
performance.
Analytical Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
This
study
presents
a
novel
spectroelectrochemical
fiber-optic
sensor
method
utilizing
surfactant-induced
electrostatic
films
to
enhance
sensitivity
and
efficiency
in
detecting
dyes
sulfide
ion
for
environmental
monitoring.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 16, 2025
Abstract
Fibrotic
diseases,
such
as
pulmonary
fibrosis,
pose
significant
challenges
in
both
research
and
treatment.
To
address
the
limitations
of
existing
systems,
a
novel
collision‐based
spheroid
mechanical
property
assessment
system
is
developed.
The
utilizes
inertial
fluid
dynamics
to
induce
controlled
collisions
through
uniformly
sized
spheroids,
allowing
strain
be
measured
via
high‐speed
cameras.
In
this
study,
first
validated
using
HEK293T
spheroids
optimize
flow
velocity,
followed
by
an
analysis
deformability
differences
between
two
cell
types
related
fibrosis
(Calu‐1
MRC‐5).
A
co‐culture
model
comprising
lung
cells,
endothelial
fibroblast
different
rations
further
developed,
depending
on
composition
observed.
Finally,
are
treated
with
TGF‐β1(Transforming
Growth
Factor‐β1),
factor
known
activate
cells
excessive
extra
cellular
matrix
(ECM)
accumulation,
Nintedanib,
anti‐fibrotic
drug,
assess
changes
properties.
These
results
effectively
reflect
properties
driven
cell‐cell
cell‐ECM
interactions
highlight
correlation
mechanics
progression
fibrotic
disease.
This
not
only
contributes
deeper
understanding
but
also
serves
powerful
platform
for
accelerating
development
therapies.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(3), P. 1183 - 1191
Published: March 3, 2023
Cellular
mechanical
property
analysis
reflecting
the
physiological
and
pathological
states
of
cells
plays
a
crucial
role
in
assessing
quality
stored
blood.
However,
its
complex
equipment
needs,
operation
difficulty,
clogging
issues
hinder
automated
rapid
biomechanical
testing.
Here,
we
propose
promising
biosensor
assisted
by
magnetically
actuated
hydrogel
stamping
to
fulfill
it.
The
flexible
magnetic
actuator
triggers
collective
deformation
multiple
light-cured
hydrogel,
it
allows
for
on-demand
bioforce
stimulation
with
advantages
portability,
cost-effectiveness,
simplicity
operation.
manipulated
cell
processes
are
captured
integrated
miniaturized
optical
imaging
system,
cellular
parameters
extracted
from
images
real-time
intelligent
sensing.
In
this
work,
30
clinical
blood
samples
different
storage
durations
(<14
days
>14
days)
were
tested.
A
deviation
3.3%
differentiation
system
compared
physician
annotation
demonstrated
feasibility.
This
should
broaden
application
assays
diverse
settings.
