Selenium
nanoparticles
(SeNPs)
exhibit
significant
potential
in
biomedical
applications
due
to
their
antimicrobial,
anticancer,
and
anti-inflammatory
properties.
In
this
study,
we
synthesized
biocompatible
SeNPs
employed
confocal
Raman
microspectroscopy
map
distribution
within
human
dermal
fibroblast
(HDF)
cells.
possess
a
distinctive
band
placed
outside
the
cellular
fingerprint
region,
which
facilitates
its
detection
precise
imaging.
Viability
assays
revealed
that
cytotoxic
effects
only
at
highest
concentrations
for
long
exposure
times
while
resulting
no
harmful
during
all
of
other
treatments.
For
first
time,
achieved
three-dimensional
(3D)
mapping
cells,
providing
insights
into
penetration.
Additionally,
two-dimensional
(2D)
performed
different
sublethal
demonstrated
dynamic
uptake
confirmed
internalization.
These
findings
highlight
effectiveness
imaging
therapeutic
applications,
offering
an
additional
approach
studying
nanoparticle–cell
interactions.
TrAC Trends in Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
153, P. 116623 - 116623
Published: April 12, 2022
The
application
of
Raman
spectroscopic
methods
in
life
science
applications
has
entered
a
new
era
due
to
advances
instrumentation,
miniaturization
and,
most
importantly,
increased
interdisciplinary
dialogue
between
spectroscopists
and
end
users
such
as
clinicians
or
pharmacists.
Advances
laser
sampling
technologies,
optical
instruments
data
analysis
provide
possibilities
for
sensing
chemical
imaging.
based
technologies
are
being
driven
by
these
developments,
opening
advanced
pharmaceutical
development
manufacturing.
Due
to,
non-destructive
label
free
quality
testing
drug
formulations,
is
particular
interest
process-related
aspects
bioprocessing.
trend
personalized
medicine,
particularly
pediatric
geriatric
the
number
prescriptions
individually
tailored
medicines
manufactured
public
pharmacies.
This
article
discusses
trends
updates
analytics
how
information
can
create
value
modern
processes.
first
section
reviews
modalities
that
have
great
potential
on-site
industry
well
research
development.
second
provides
an
overview
innovative
devices
many
terms
analytical
capabilities
sample
properties.
Photonic
been
important
driver
breakthroughs
last
decade
help
standardize
spectral
analysis,
which
discussed
third
section.
In
we
discuss
translate
innovations
into
user-friendly
applications.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(6), P. 3507 - 3515
Published: Feb. 1, 2023
ATP
and
reactive
oxygen
species
(ROS)
are
considered
significant
indicators
of
cell
apoptosis.
However,
visualizing
the
interplay
between
apoptosis-related
ROS
is
challenging.
Herein,
we
developed
a
metal–organic
framework
(MOF)-based
nanoprobe
for
an
apoptosis
assay
using
duplex
imaging
cellular
ROS.
The
was
fabricated
through
controlled
encapsulation
gold
nanorods
with
thin
zirconium-based
MOF
layer,
followed
by
modification
ROS-responsive
molecules
2-mercaptohydroquinone
6-carboxyfluorescein-labeled
aptamer.
enables
visualization
via
fluorescence
surface-enhanced
Raman
spectroscopy,
respectively,
avoiding
mutual
interference
that
often
occurs
in
single-mode
methods.
Moreover,
dual-modal
effectively
showed
dynamic
cancer
cells
treated
various
drugs,
revealing
their
pathways
interactions
differ
from
those
under
normal
conditions.
This
study
provides
method
studying
relationship
energy
metabolism
redox
homeostasis
processes.
The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
902, P. 166003 - 166003
Published: Aug. 6, 2023
The
accumulation
of
microplastics
(MPs)
in
the
environment
as
well
their
presence
foods
and
humans
highlight
urgent
need
for
studies
on
effects
these
particles
humans.
Polylactic
acid
(PLA)
is
most
widely
used
bioplastic
food
industry
medical
field.
Despite
its
biodegradability,
biocompatibility,
"Generally
Recognized
As
Safe"
(GRAS)
status,
recent
animal
model
have
shown
that
PLA
MPs
can
alter
intestinal
microbiota;
however,
to
date,
no
been
reported
possible
gut
health
consequences
intake
by
This
work
simulates
ingestion
a
realistic
daily
amount
pass
through
gastrointestinal
tract
combining
INFOGEST
method
simgi®
evaluate
human
colonic
microbiota
composition
(16S
rRNA
gene
sequencing
analysis)
metabolic
functionality
(lactic
short-chain
fatty
acids
(SCFA)
production).
Although
did
not
clearly
microbial
community
homeostasis,
increased
Bifidobacterium
levels
tended
increase
millimetric
particles.
Furthermore,
shifts
detected
at
functional
level
suggest
an
alteration
metabolism,
biotransformation
community.
Raman
spectroscopy
field
emission
scanning
electron
microscopy
(FESEM)
characterization
revealed
morphological
changes
after
gastric
phase
digestion,
adhesion
organic
matter
biofilm,
with
surface
biodegradation,
phases.
With
this
evidence
emerging
use
bioplastics,
understanding
impact
potential
biodegradation
digestion
merits
critical
investigation.
Smart Medicine,
Journal Year:
2024,
Volume and Issue:
3(1)
Published: Jan. 30, 2024
Cancer
remains
a
major
global
health
threat
necessitating
the
multipronged
approaches
for
its
prevention
and
management.
Traditional
in
form
of
chemotherapy,
surgery,
radiotherapy
are
often
encountered
with
poor
patient
outcomes
evidenced
by
high
mortality
morbidity,
compelling
need
precision
medicine
cancer
patients
to
enable
personalized
targeted
treatment.
There
has
been
an
emergence
smart
multimodal
theranostic
nanoformulation
triple
combination
therapy
last
few
years,
which
dramatically
enhances
overall
safety
vivo
potential
clinical
applications
minimal
toxicity.
However,
it
is
imperative
gain
insight
into
limitations
this
system
terms
translation,
cost-effectiveness,
accessibility,
multidisciplinary
collaboration.
This
review
paper
aims
highlight
compare
impact
recent
nanoformulations
therapeutics
single
nanocarrier
effective
management
provide
new
dimension
diagnostic
treatment
simultaneously.
npj Imaging,
Journal Year:
2025,
Volume and Issue:
3(1)
Published: Jan. 31, 2025
Biological
images
captured
by
microscopes
are
characterized
heterogeneous
signal-to-noise
ratios
(SNRs)
due
to
spatially
varying
photon
emission
across
the
field
of
view
convoluted
with
camera
noise.
State-of-the-art
unsupervised
structured
illumination
microscopy
(SIM)
reconstruction
methods,
commonly
implemented
in
Fourier
domain,
often
do
not
accurately
model
this
Such
methods
therefore
suffer
from
high-frequency
artifacts,
user-dependent
choices
smoothness
constraints
making
assumptions
on
biological
features,
and
unphysical
negative
values
recovered
fluorescence
intensity
map.
On
other
hand,
supervised
algorithms
rely
large
datasets
for
training,
require
retraining
new
sample
structures.
Consequently,
achieving
high
contrast
near
maximum
theoretical
resolution
an
unsupervised,
physically
principled
manner
remains
open
problem.
Here,
we
propose
Bayesian-SIM
(B-SIM),
a
Bayesian
framework
quantitatively
reconstruct
SIM
data,
rectifying
these
shortcomings
incorporating
known
noise
sources
spatial
domain.
To
accelerate
process,
use
finite
extent
point-spread-function
devise
parallelized
Monte
Carlo
strategy
involving
chunking
restitching
inferred
intensity.
We
benchmark
our
both
simulated
experimental
images,
demonstrate
improved
permitting
feature
recovery
at
up
25%
shorter
length
scales
over
state-of-the-art
high-
low
SNR.
B-SIM
enables
quantitative,
accurate
without
need
labeled
training
democratizing
high-quality
expands
capabilities
live-cell
lower
SNR,
potentially
revealing
features
previously
inaccessible
regimes.
Analytica Chimica Acta,
Journal Year:
2024,
Volume and Issue:
1295, P. 342312 - 342312
Published: Feb. 2, 2024
Raman
spectroscopists
are
familiar
with
the
challenge
of
dealing
spikes
caused
by
cosmic
rays.
These
artifacts
may
lead
to
errors
in
subsequent
data
processing
steps,
such
as
for
example
calibration,
normalization
or
spectral
search.
Spike
removal
is
therefore
a
fundamental
step
pre-treatment,
but
access
publicly
accessible
code
spike
tools
limited,
and
their
performance
spectra
correction
often
unknown.
Therefore,
there
need
development
testing
open-source
easy-to-implement
algorithms
that
improve
workflow.
