Published: April 4, 2024

Neurotransmitters are released by neuronal cells to exchange information. Resolving their spatiotemporal patterns is crucial understand chemical neurotransmission. Here, we present a ratiometric sensor for the neurotransmitter dopamine that combines Egyptian Blue (CaCuSi4O10) nanosheets (EB-NS) and single-walled carbon nanotubes (SWCNTs). They both fluoresce in near infrared (NIR), which beneficial due ultra-low background phototoxicity. (GT)10-DNA functionalized monochiral (6,5)-SWCNTs increase fluorescence (1000 nm) response dopamine, while EB-NS serves as stable reference (936 nm). A robust imaging scheme implemented directing these signals on two different NIR sensitive cameras. Additionally, demonstrate stability against mechanical perturbations image release from differentiated dopaminergic Neuro 2a cells. Therefore, this technique enables non-invasive of cellular responses.

Language: Английский