Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(12), P. 7953 - 8039

Published: June 1, 2023

Whole blood, as one of the most significant biological fluids, provides critical information for health management and disease monitoring. Over past 10 years, advances in nanotechnology, microfluidics, biomarker research have spurred development powerful miniaturized diagnostic systems whole blood testing toward goal monitoring treatment. Among techniques employed whole-blood diagnostics, electrochemical biosensors, known to be rapid, sensitive, capable miniaturization, reagentless washing free, become a class emerging technology achieve target detection specifically directly complex media, e.g., or even living body. Here we are aiming provide comprehensive review summarize over decade sensors analysis. Further, address remaining challenges opportunities integrate sensing platforms.

Language: Английский

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Comprehensive review on the electrochemical biosensors of different breast cancer biomarkers

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 365, P. 131944 - 131944

Published: April 25, 2022

Language: Английский

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Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 333 - 333

Published: March 1, 2023

With its fatal effects, cancer is still one of the most important diseases today’s world. The underlying fact behind this scenario probably due to late diagnosis. That why necessity for detection different types obvious. Cancer studies including diagnosis and therapy have been laborious tasks. Since early significantly affects following steps, very important. Despite researchers’ best efforts, accurate rapid challenging difficult investigate. It known that electrochemical techniques successfully adapted into field. Electrochemical sensor platforms are brought together with excellent selectivity biosensing elements, such as nucleic acids, aptamers or antibodies, put forth successful outputs. One remarkable achievements these biomolecule-attached sensors their lack need additional labeling which bring extra burdens interference effects demanding modification protocols. In review, we aim outline label-free use methods acquire signals. classification sensing generally presented according recognition element, recent by using attractive substrates described in detail. addition, current challenges discussed.

Language: Английский

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Dual-Mode Sensing Platform for Cancer Antigen 15-3 Determination Based on a Silica Nanochannel Array Using Electrochemiluminescence and Electrochemistry

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 317 - 317

Published: Feb. 24, 2023

An electrochemiluminescence-electrochemistry (ECL-EC) dual-mode sensing platform based on a vertically-ordered mesoporous silica films (VMSF) modified electrode was designed here for the sensitive and selective determination of cancer antigen 15-3 (CA 15-3), specific biomarker breast cancer. VMSF assembled through rapid electrochemically assisted self-assembly (EASA) method plays crucial role in signal amplification via strong electrostatic interaction with positively charged bifunctional probe Ru(bpy)32+. To construct biorecognition interface, epoxy functional silane linked to surface further covalent immobilization antibody. As benefit from combination antibody, non-conductive immunocomplex layer formed presence CA 15-3, leading hinderance mass electron transfer probes. Based this strategy, ranging 0.1 mU/mL 100 LOD 9 μU/mL ECL mode, 10 200 U/mL 5.4 EC achieved. The proposed immunosensor successfully employed detection human serum without tedious pretreatment.

Language: Английский

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2D Materials in Advanced Electronic Biosensors for Point‐of‐Care Devices

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 18, 2024

Since two-dimensionalal (2D) materials have distinct chemical and physical properties, they are widely used in various sectors of modern technologies. In the domain diagnostic biodevices, particularly for point-of-care (PoC) biomedical diagnostics, 2D-based field-effect transistor biosensors (bio-FETs) demonstrate substantial potential. Here, this review article, operational mechanisms detection capabilities biosensing devices utilizing graphene, transition metal dichalcogenides (TMDCs), black phosphorus, other 2D addressed detail. The incorporation these into FET-based offers significant advantages, including low limits (LOD), real-time monitoring, label-free diagnosis, exceptional selectivity. also highlights diverse applications biosensors, ranging from conventional to wearable devices, underscoring versatility material-based FET devices. Additionally, provides a comprehensive assessment limitations challenges faced by along with insights future prospects advancements. Notably, detailed comparison is tabulated platforms their working mechanisms. Ultimately, aims stimulate further research innovation field while educating scientific community about latest advancements materials-based biosensors.

Language: Английский

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Review—Systematic Review on Electrochemical Biosensing of Breast Cancer miRNAs to Develop Alternative DCIS Diagnostic Tool

ECS Sensors Plus, Journal Year: 2022, Volume and Issue: 1(2), P. 021602 - 021602

Published: June 1, 2022

Till date, no systematic review is conducted on electrochemical biosensing of multiplexed breast cancer miRNAs to identify their suitability as an alternative diagnostic tool for ductal carcinoma in situ (DCIS). Original articles published English from PUBMED, Science Direct, Scopus, MEDLINE, Cochrane Library, National Centre Biotechnology Information, and Google scholar during Jan 2012–Feb 2022 were searched using set inclusion criteria. The Preferred Reporting Items Systematic Reviews Meta-analysis (PRISMA) 2020 guidelines was followed report this review. Methodological quality the included studies evaluated Risk Bias (ROB) assessment Out 1973 screened articles, 17 eligible wherein, 1 study (5.88%) involving generic neutravidin modified nanolabel probe biotintylated molecular beacons immobilized metal nanoparticles prepared one pot assay detect 21 141 had low ROB 16 (94.12%) medium ROB. Medium majority reveals its limited evidence conclude diagnosing DCIS miRNAs. High with inter assays validation are extensively needed development via

Language: Английский

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Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(1), P. 420 - 432

Published: Jan. 10, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTElectrochemical Aptamer-Based Sensors with Tunable Detection RangeJing PanJing PanState Key Laboratory of Biogeology and Environmental Geology, Faculty Materials Science Chemistry, China University Geosciences, Wuhan 430074, ChinaMore by Jing PanView Biography, Wenxia XuWenxia XuState XuView Wanlu LiWanlu LiState LiView Shuwen ChenShuwen ChenState ChenView Yu DaiYu DaiState DaiView Shanwu YuShanwu YuState YuView Qitao Zhou*Qitao ZhouState China*[email protected]More ZhouView Fan XiaFan XiaState XiaView Biographyhttps://orcid.org/0000-0001-7705-4638Cite this: Anal. Chem. 2023, 95, 1, 420–432Publication Date (Web):January 10, 2023Publication History Received12 October 2022Published online10 January 2023Published inissue 10 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c04498https://doi.org/10.1021/acs.analchem.2c04498review-articleACS PublicationsCopyright © 2023 American Chemical SocietyRequest reuse permissionsArticle Views2207Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Electrodes,Particulate matter,Peptides proteins,Redox reactions,Sensors Get e-Alerts

Language: Английский

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An electrochemical biosensor for HER2 detection in complex biological media based on two antifouling materials of designed recognizing peptide and PEG

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1252, P. 341075 - 341075

Published: March 8, 2023

Language: Английский

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Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis

Molecules, Journal Year: 2023, Volume and Issue: 28(18), P. 6719 - 6719

Published: Sept. 20, 2023

Graphene is an emerging nanomaterial increasingly being used in electrochemical biosensing applications owing to its high surface area, excellent conductivity, ease of functionalization, and superior electrocatalytic properties compared other carbon-based electrodes nanomaterials, enabling faster electron transfer kinetics higher sensitivity. biosensors may have the potential enable rapid, sensitive, low-cost detection cancer biomarkers. This paper reviews early-stage research proof-of-concept studies on development graphene for future diagnostic applications. Various synthesis methods are outlined along with common functionalization approaches using polymers, biomolecules, synthetic chemistry facilitate immobilization recognition elements improve performance. Major sensor configurations including field-effect transistors, modified nanocomposites, 3D networks highlighted their principles operation, advantages, capabilities. Strategies biorecognition like antibodies, aptamers, peptides, DNA/RNA probes onto platforms impart target specificity summarized. The use labels, hybrid nanocomposites graphene, chemical modification signal enhancement also discussed. Examples provided illustrate sensitive a broad range biomarkers proteins, circulating tumor cells, DNA mutations, non-coding RNAs miRNA, metabolites, glycoproteins. Current challenges opportunities elucidated guide ongoing efforts towards transitioning from promising lab tools into mainstream clinical practice. Continued addressing issues reproducibility, stability, selectivity, integration, validation, regulatory approval could wider adoption. Overall, present powerful versatile diagnosis at point care.

Language: Английский

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Homogeneous Electrochemical Aptamer Sensor Based on Two-Dimensional Nanocomposite Probe and Nanochannel Modified Electrode for Sensitive Detection of Carcinoembryonic Antigen

Molecules, Journal Year: 2023, Volume and Issue: 28(13), P. 5186 - 5186

Published: July 3, 2023

A rapid and convenient homogeneous aptamer sensor with high sensitivity is highly desirable for the electrochemical detection of tumor biomarkers. In this work, a demonstrated based on two-dimensional (2D) nanocomposite probe nanochannel modified electrode, which can realize sensitive carcinoembryonic antigen (CEA). Using π-π stacking electrostatic interaction, CEA (Apt) cationic redox (hexaammineruthenium(III), Ru(NH3)63+) are co-loaded graphite oxide (GO), leading to 2D (Ru(NH3)63+/Apt@GO). Vertically ordered mesoporous silica-nanochannel film (VMSF) easily grown supporting indium tin (ITO) electrode (VMSF/ITO) using assisted self-assembly (EASA) method within 10 s. The ultrasmall nanochannels VMSF exhibits enrichment towards Ru(NH3)63+ size exclusion material. When added in Ru(NH3)63+/Apt@GO solution, DNA recognizes binds releases be enriched detected by VMSF/ITO electrodes. Based mechanism, an ranging from 60 fg/mL 100 ng/mL limit (LOD) 14 fg/mL. Detection human serum also realized. constructed system does not require fixation recognitive surface or magnetic separation before detection, demonstrating potential applications rapid, sensing

Language: Английский

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