Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(49), P. 19847 - 19854
Published: Nov. 28, 2024
Aging
is
a
critical
global
issue
that
contributes
to
the
high
incidence
of
Alzheimer's
disease
(AD).
Blood
screening
emerges
as
most
promising
measure
for
early
diagnosis
and
intervention
AD
due
its
noninvasive
low
cost.
However,
practical
application
blood
confronts
two
significant
challenges.
First,
blood-brain
barrier,
concentration
biomarkers
in
much
lower
than
cerebrospinal
fluid.
Second,
simultaneous
quantitative
analysis
multiple
necessary
specificity
individual
biomarkers.
Herein,
we
propose
DNAzyme-based
3D
DNA
walkers
sensitive
multiplex
detection
five
AD-associated
miRNA
biomarkers:
hsa-miR-125b,
hsa-miR-342-3p,
hsa-miR-29b,
hsa-miR-191-5p,
hsa-miR-7d-5.
The
provide
highly
efficient
autonomous
amplification
minimal
biomarkers'
quantities.
walking-released
metal
isotopes
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(28), P. 11566 - 11571
Published: June 28, 2024
DNA
walking
machines
have
achieved
significant
breakthroughs
in
areas
such
as
biosensing,
bioimaging,
and
early
cancer
diagnosis,
facilitated
by
the
self-assembly
of
or
its
combination
with
other
materials,
magnetic
beads
metal
nanoparticles.
However,
current
machine
strategies
are
constantly
challenged
inadequate
analytical
sensitivity,
while
sophisticated
signal
amplification
procedures
often
indispensable.
Single-particle
inductively
coupled
plasma
mass
spectrometry
(SP-ICPMS)
provides
superior
sensitivity
can
effectively
discriminate
between
background
noise
detected
signals
due
to
large
number
atoms
a
nanoparticle
concentrating
effect
single
detection.
In
this
study,
we
present
novel
approach
utilizing
counting
duplex-specific
nuclease
(DSN)-assisted
construct
3D
for
detecting
aggressive
prostate
(PCa)
biomarker
miRNA-200c.
The
proposed
strategy
showed
an
improvement
detection
limit
(LOD)
0.93
pM
(28
amol)
was
successfully
applied
human
serum
samples.
To
best
our
knowledge,
is
first
report
study.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(29), P. 10524 - 10530
Published: July 13, 2022
In
this
work,
an
intelligent
multiregion
linear
DNA
walker
(MLDW)
with
a
high
walking
rate
and
amplification
efficiency
was
explored
for
ultrasensitive
detection
of
miRNA.
Significantly,
amounts
functional
domain
could
be
concentrated
in
long
obtained
by
the
target
miRNA-mediated
rolling-circle
to
simultaneously
increase
local
concentration
collision
probability,
resulting
obviously
improved
reaction
rate.
Impressively,
MLDW
can
accomplish
within
30
min,
which
is
at
least
4
times
beyond
that
traditional
single-leg
multiple-leg
walkers.
As
proof
concept,
high-efficiency
used
develop
electrochemical
biosensing
platform
miRNA-21
low
limit
down
36
aM.
Therefore,
we
designed
puts
forward
innovative
insight
construct
nanodevice
promote
investigation
inherent
performance
nucleic
acid
signal
ultimate
application
biomolecules
clinical
disease
diagnosis.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(37), P. 12934 - 12941
Published: Sept. 7, 2022
Inductively
coupled
plasma–mass
spectrometry
(ICP–MS)
with
elemental
labeling
is
a
promising
strategy
for
multiplex
microRNA
(miRNA)
analysis.
However,
it
still
challenging
specific
analysis
of
multiple
miRNAs
high
homology,
and
the
development
assays
always
limited
by
complexity
sequence
design.
Herein,
simple
direct
ICP–MS-based
assay
was
developed
simultaneous
detection
three
combining
lanthanide
entropy-driven
catalytic
(EDC)
amplification.
Owing
to
specificity
EDC
nucleic
acid
recognition,
able
differentiate
single-base
mutation
in
each
circuit.
A
universal
biotin-labeled
DNA
strand
designed
hybridize
substrates
circuits,
targeting
miRNA-21,
miRNA-155,
miRNA-10b,
respectively.
All
were
loaded
on
surface
streptavidin
magnetic
beads.
In
presence
target
miRNA,
reaction
initiated,
dissociated,
continuously
releasing
reporter
strands
that
labeled
lanthanides
(Tb/Ho/Lu).
After
separation,
supernatant
containing
released
introduced
into
an
ICP–MS
system
159Tb/165Ho/175Lu
quantification
The
limits
7.4,
7.5,
11
pmol
L–1
Overall,
this
study
provides
powerful
miRNAs,
advantages
flexible
probe
design,
good
sensitivity,
excellent
specificity.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(49), P. 17248 - 17254
Published: Nov. 30, 2022
The
occurrence
of
cancers
is
often
accompanied
by
the
abnormal
expression
several
sorts
biomarkers
(e.g.,
nucleic
acids
and
proteins).
multiplex
assessment
them
would
substantially
aid
in
early
detection
precise
diagnosis,
which
hampered
their
different
schemes,
reaction
matrix
reagents,
spectral
overlapping.
Herein,
we
propose
a
simple
sensitive
mass
spectrometric
method
for
acid
protein,
liver
cancer-related
miRNA
223
alpha-fetoprotein
(AFP)
were
selected
as
model
analytes.
self-amplification
effect
metal
atom-based
nanoparticle
probes
can
provide
high
sensitivity
complex
serum
samples
without
any
additional
amplification
procedure.
limits
simultaneous
AFP
103
(2.1
pM)
219
amol
(0.15
ng/mL),
respectively,
with
specificity
selectivity.
proposed
potentially
useful
rapid
screening
cancers.
Nano Letters,
Journal Year:
2023,
Volume and Issue:
23(5), P. 1820 - 1829
Published: Feb. 15, 2023
Multiplexed
profiling
of
RNAs
aids
in
a
comprehensive
understanding
multiparameter-defined
cellular
processes
and
pathological
states.
We
herein
present
mass
nanotags-enabled
interfacial
assembly
system
(MNTs-AS)
with
parallel
amplification
motors
for
simultaneous
assaying
multiple
biosystems
by
matrix-assisted
laser
desorption/ionization
spectrometry
(MALDI
MS).
Four
kinds
MNTs
encoding
corresponding
RNA
can
be
cyclically
assembled
on
magnetic
beads
target-triggered
catalytic
hairpin
(CHA)
machineries
nanointerfaces,
generating
multiplexed
amplified
characteristic
ion
signals
assigned
to
target
upon
MALDI
MS
interrogation.
By
virtue
high
sensitivity
multiplexing
capability,
the
MNTs-AS-based
assay
allows
precision
subtyping
diverse
breast
cancer
cells
their
exosomes
miRNA-21,
miRNA-373,
miRNA-155,
manganese
superoxide
dismutase
mRNA
via
single
inquiry.
This
method
provides
promising
tool
unraveling
RNA-involved
biological
events
fundamental
research
distinguishing
different
subtypes
clinical
practice.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 6, 2025
DNA
logic
circuits
have
gained
great
success
in
the
past,
thanks
to
their
distinct
performance
regarding
scalability
and
correctness
of
computation.
However,
there
are
still
two
challenges
often
considered
for
circuit-based
First,
mainstream
optical
probes
subject
spectral
overlapping
interference
complex
multitask
analysis
outputs.
Second,
absolute
quantification
results
traceable
primary
international
system
units
mission
impossible,
especially
interlaboratory
comparisons
quality
assurances.
Herein,
we
constructed
encoded
with
lanthanide
isotopes
decoded
by
elemental
mass
spectrometry.
The
155Gd-enriched
isotope
145Nd-enriched
were
incorporated
dilution-based
microRNAs.
proposed
isotopic
greatly
enhance
multiplexity
computation
accuracy,
which
poses
a
potential
cancer
biomarker-related
diagnosis.
