Construction of a Spatial-Confined Self-Stacking Catalytic Circuit for Rapid and Sensitive Imaging of Piwi-Interacting RNA in Living Cells

Nano Letters, Journal Year: 2024, Volume and Issue: 24(28), P. 8732 - 8740

Published: July 3, 2024

Piwi-interacting RNAs (piRNAs) are small noncoding that repress transposable elements to maintain genome integrity. The canonical catalytic hairpin assembly (CHA) circuit relies on random collisions of free-diffused reactant probes, which substantially slow down reaction efficiency and kinetics. Herein, we demonstrate the construction a spatial-confined self-stacking for rapid sensitive imaging piRNA in living cells based intramolecular intermolecular hybridization-accelerated CHA. We rationally design 3WJ probe not only accelerates kinetics by increasing local concentration probes but also eliminates background signal leakage caused cross-entanglement preassembled probes. This strategy achieves high sensitivity good specificity with shortened assay time. It can quantify intracellular expression at single-cell level, discriminate tissues breast cancer patients healthy persons, situ image cells, offering new approach early diagnosis postoperative monitoring.

Language: Английский

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DNA Nanostructure Disintegration-Assisted SPAAC Ligation for Electrochemical Biosensing

Nano Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

MicroRNAs (MiRNAs) are valuable biomarkers for the diagnosis and prognosis of diseases. The development reliable assays is an urgent pursuit. We herein fabricate a novel electrochemical sensing strategy based on conformation transitions DNA nanostructures click chemistry. Duplex-specific nuclease (DSN)-catalyzed reaction first used disintegration triangular pyramid frustum (DNA TPF). A triangle formed, which in turn assists strain-promoted alkyne-azide cycloaddition (SPAAC) to localize single-stranded probes (P1). After SPAAC ligation, multiple hairpins spontaneously folded, labeled species dragged near electrode interface. By recording analyzing responses, highly sensitive biosensor established, exhibits high sensitivity reproducibility. Clinical applications have been verified with good stability. This relies integration chemistry, may inspire further designs nanotechnology clinical

Language: Английский

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Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

Chemical Research in Chinese Universities, Journal Year: 2024, Volume and Issue: 40(2), P. 237 - 254

Published: March 20, 2024

Language: Английский

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Material-enhanced biosensors for cancer biomarkers detection

Microchemical Journal, Journal Year: 2023, Volume and Issue: 194, P. 109298 - 109298

Published: Sept. 3, 2023

Language: Английский

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Hierarchical DNA Octahedral Nanoplatform for in Situ Biosensing and Clinical Monitoring of Acute Lymphoblastic Leukemia

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Developing nanoscale platforms with high integration, assembly efficiency, and structural stability for performing complex computations in specific cells remains a significant challenge. To address this, the Three‐dimensional Hierarchical Octahedral Robotic (THOR) DNA nanoplatform is introduced, which integrates targeting, logic computation, sensing modules within single framework. This specifically binds to cancer cell surface proteins, releasing aptamer‐linked fuel chains initiate subsequent computational processes. Three gates efficiently compute any arbitrary binary combination of target proteins. The module employs catalytic hairpin detecting miRNAs sensitivity. THOR demonstrates robust functionality both vitro situ. As proof‐of‐concept, this distinguish acute lymphoblastic leukemia (ALL) patients from other subtypes healthy participants, achieving 100% accuracy applied. Additionally, approach reliably monitored therapeutic progress ALL patients, showing strong concordance bone marrow smear results. platform highlights feasibility constructing reliable, hierarchical, multifunctional analytical system based on polyhedron. It offers promising auxiliary tool clinical diagnostics monitoring.

Language: Английский

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Computer-Aided Design of 3D Non-Enzymatic Catalytic Cascade Systems for In Situ Multiplexed mRNA Imaging in Single-Cells

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

mRNA, a critical biomarker for various diseases and promising target cancer therapy, is central to biological medical research. However, the development of multiplexed approaches in situ monitoring mRNA live cells are limited by their reliance on enzyme-based signal amplification, challenges with diffusion, complexity nucleic acid design. In this study, we introduce nonenzymatic catalytic DNA assembly (NEDA) technique address these limitations. NEDA facilitates precise imaging intracellular assembling three free hairpin amplifiers into low-mobility, three-dimensional spherical structure. This approach also enables simultaneous detection four distinct targets via combination fluorescent signals, limit as low 141.2 pM mRNA. To enhance efficiency design, employed computer-aided design (CAD) rapidly generate feasible sequences highly detection. By integrating machine learning algorithms, achieved impressive accuracy nearly 96.66% distinguishing multiple cell types 87.80% identifying same type under different drug stimulation conditions. Notably, our platform can identify stimuli similar mechanisms action, highlighting its potential development. 3D sensing strategy CAD not only enhances ability image simultaneously but provides novel efficient molecular diagnostics personalized therapy.

Language: Английский

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Hourglass-inspired total analysis system for the sensitive colorimetric detection of exosomal miRNA using the CRISPR-Cas12a system with isothermal amplification

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162729 - 162729

Published: April 1, 2025

Language: Английский

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Quality Control of Mass‐Encoded Nanodevices by Compartmented DNA Origami Frames for Precision Information Coding and Logic Mapping

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(4)

Published: Dec. 1, 2023

Abstract Encoded nanostructures afford an ideal platform carrying multi‐channel signal components for multiplexed assay and information security. However, with the demand on exclusivity reproducibility of coding signals, precise control structure composition nanomaterials featuring fully distinguishable signals remains challenging. By using multiplexing capability mass spectrometry (MS) spatial addressability DNA origami nanostructures, we herein propose a quality methodology constructing mass‐encoded nanodevices (namely MNTs‐TDOFs) in scaffold compartmented tetrahedral frames (TDOFs), which arrangement stoichiometry four types nanotags (MNTs) can be finely regulated customized to generate characteristic MS patterns. The programmability combinatorial MNTs orthogonality individual compartments allows further evolution MNTs‐TDOFs static tagging agents dynamic nanoprobes labeling sensing multiple targets. More importantly, at single TDOF level ensures constancy prescribed outputs, by high‐capacity system was established secure encryption decryption. In addition outputs parallel, could also map logic circuits interconnected complexity events c‐Met recognition dimerization cell surface signaling regulation interrogation.

Language: Английский

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Smart DNA sensors‐based molecular identification for cancer subtyping

Smart Molecules, Journal Year: 2023, Volume and Issue: 1(3)

Published: Dec. 1, 2023

Abstract Molecular subtyping of cancer can greatly help to understand the development disease and predict tumor behavior. Exploring detection methods for precise is appealing prognosis personalized therapy. During past decades, DNA‐based biosensors have exhibited great potential in diagnosis due their structural programmability functional diversity. Despite encouraging progress that has been made, there remains an issue improving accuracy sensitivity complex process disease, especially preclinical or clinical applications. To accelerate DNA sensors identification subtypes, this review, we summarized advances molecular by analyzing heterogeneity categories levels biomarkers between subtypes. The strategies toward genomic proteomic cells on cell surface, as well excretions including extracellular vesicles (EVs) microRNA (miRNAs) serum, are summarized. Current challenges opportunities field also discussed.

Language: Английский

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DNA Walker-Driven Mass Nanotag Assembly System for Simultaneously Profiling Dual Markers of Oxidative Stress at Different Cellular Locations

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(21), P. 8754 - 8762

Published: May 13, 2024

Simultaneous profiling of redox-regulated markers at different cellular sublocations is great significance for unraveling the upstream and downstream molecular mechanisms oxidative stress in living cells. Herein, by synchronizing dual target-triggered DNA machineries one nanoentity, we engineered a walker-driven mass nanotag (MNT) assembly system (w-MNT-AS) that can be sequentially activated stress-associated mucin 1 (MUC1) apurinic/apyrimidinic endonuclease (APE1) from plasma membrane to cytoplasm induce recycled MNTs multiplex detection two matrix-assisted laser desorption ionization spectrometry (MALDI MS). In working cascade, sensing process governs separate activation w-MNT-AS MUC1 APE1 diverse locations, while contributes parallel amplification ion signal characteristic tags. this manner, differences between MCF-7, HeLa, HepG2, L02 cells expression cytoplasmic were fully characterized. Furthermore, level dynamics caused exogenous H2O2, doxorubicin, simvastatin comprehensively demonstrated tracking fate across locations. The proposed coupled MS method provides an effective route probe multiple functional molecules lie locations participating same event, facilitating mechanistic studies on response other disease-related processes.

Language: Английский

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