Nanocrystals for Deep-Tissue In Vivo Luminescence Imaging in the Near-Infrared Region

Chemical Reviews, Journal Year: 2023, Volume and Issue: 124(2), P. 554 - 628

Published: Nov. 22, 2023

In vivo imaging technologies have emerged as a powerful tool for both fundamental research and clinical practice. particular, luminescence in the tissue-transparent near-infrared (NIR, 700–1700 nm) region offers tremendous potential visualizing biological architectures pathophysiological events living subjects with deep tissue penetration high contrast owing to reduced light–tissue interactions of absorption, scattering, autofluorescence. The distinctive quantum effects nanocrystals been harnessed achieve exceptional photophysical properties, establishing them promising category luminescent probes. this comprehensive review, between light tissues, well advantages NIR imaging, are initially elaborated. Subsequently, we focus on achieving improved by optimizing performance nanocrystal fluorophores. ingenious design strategies probes discussed, along their respective biomedical applications versatile modalities. Finally, thought-provoking reflections challenges prospects future translation nanocrystal-based wisely provided.

Language: Английский

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Colorimetric sensing for translational applications: from colorants to mechanisms

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(15), P. 7681 - 7741

Published: Jan. 1, 2024

This review summarizes insights into colorant selection and signal mechanisms for the development of colorimetric sensing POC sensors.

Language: Английский

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Oxidative stress modulating nanomaterials and their biochemical roles in nanomedicine

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(10), P. 1630 - 1682

Published: Jan. 1, 2024

Many pathological conditions are predominantly associated with oxidative stress, arising from reactive oxygen species (ROS); therefore, the modulation of redox activities has been a key strategy to restore normal tissue functions. Current approaches involve establishing favorable cellular environment through administration therapeutic drugs and redox-active nanomaterials (RANs). In particular, RANs not only provide stable reliable means delivery but also possess capacity finely tune various interconnected components, including radicals, enzymes, proteins, transcription factors, metabolites. Here, we discuss roles that engineered play in spectrum conditions, such as cancer, neurodegenerative diseases, infections, inflammation. We visualize dual functions both generator scavenger ROS, emphasizing their profound impact on diverse The focus this review is solely inorganic (inorganic RANs). Additionally, deliberate challenges current RANs-based propose potential research directions for future clinical translation.

Language: Английский

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Advancements in semiconductor quantum dots: expanding frontiers in optoelectronics, analytical sensing, biomedicine, and catalysis

Journal of Materials Chemistry C, Journal Year: 2024, Volume and Issue: 12(28), P. 10330 - 10389

Published: Jan. 1, 2024

Different applications of semiconductor quantum dots with their unique fundamental properties.

Language: Английский

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Targeting Peptides: The New Generation of Targeted Drug Delivery Systems

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1648 - 1648

Published: June 3, 2023

Peptides can act as targeting molecules, analogously to oligonucleotide aptamers and antibodies. They are particularly efficient in terms of production stability physiological environments; recent years, they have been increasingly studied agents for several diseases, from tumors central nervous system disorders, also thanks the ability some them cross blood–brain barrier. In this review, we will describe techniques employed their experimental silico design, well possible applications. We discuss advancements formulation chemical modifications that make even more stable effective. Finally, how use could effectively help overcome various problems improve existing treatments.

Language: Английский

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Evaluating the Catalytic Efficiency of the Human Membrane-type 1 Matrix Metalloproteinase (MMP-14) Using AuNP–Peptide Conjugates

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(8), P. 4570 - 4582

Published: Feb. 20, 2023

Interactions of plasmonic nanocolloids such as gold nanoparticles and nanorods with proximal dye emitters result in efficient quenching the photoluminescence (PL). This has become a popular strategy for developing analytical biosensors relying on this process signal transduction. Here, we report use stable PEGylated nanoparticles, covalently coupled to dye-labeled peptides, sensitive optically addressable sensors determining catalytic efficiency human matrix metalloproteinase-14 (MMP-14), cancer biomarker. We exploit real-time PL recovery triggered by MMP-14 hydrolysis AuNP−peptide-dye extract quantitative analysis proteolysis kinetics. Sub-nanomolar limit detections been achieved using our hybrid bioconjugates. In addition, have used theoretical considerations within diffusion-collision framework derive enzyme substrate inhibition kinetics equations, which allowed us describe complexity irregularity enzymatic nanosurface-immobilized peptide substrates. Our findings offer great development highly detection imaging.

Language: Английский

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Construction of a Quantum-Dot-Based FRET Nanosensor through Direct Encoding of Streptavidin-Binding RNA Aptamers for N6-Methyladenosine Demethylase Detection

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(35), P. 13201 - 13210

Published: Aug. 21, 2023

N6-Methyladenosine (m6A) demethylases can catalyze the removal of methyl modification on m6A, and it is closely associated with occurrence, proliferation, differentiation, metastasis malignancies. The m6A (e.g., fat mass obesity-associated protein (FTO)) may act as a cancer biomarker are crucial for anticancer drug screening early clinical diagnosis. Herein, we demonstrate construction quantum-dot-based Förster resonance energy-transfer (FRET) nanosensor through direct encoding streptavidin-binding RNA aptamers (SA aptamers) demethylase detection. This employs multiple Cy5-molecule-labeled SA building materials to construct 605QD-RNA-Cy5 nanoassembly, exploits hinder effect upon elongation ligation reactions distinguish m6A-containing probes from demethylated probes. When present, generate probes, initiating strand extension yield complete transcription template aptamers. Subsequently, T7-assisted cascade amplification reaction activated transcribe abundant incorporation Cy5 fluorophores. Cy5-incorporated self-assembly onto streptavidin-coated 605QD surface obtain 605QD-SA aptamer-Cy5 nanoassemblies, resulting in generation distinct FRET signals. exhibits ultrahigh sensitivity excellent specificity, detect endogenous FTO at single-cell level. Furthermore, this precisely measure enzyme kinetic parameters, screen inhibitors, differentiate expression between breast patients healthy individual tissues, offering versatile platform diagnostic discovery.

Language: Английский

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Ultrasensitive quantifying copper ions in serum by tapered optical fiber fluorescent sensor based on static quenching effect

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 396, P. 134557 - 134557

Published: Sept. 5, 2023

Language: Английский

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Advancements in SARS-CoV-2 detection: Navigating the molecular landscape and diagnostic technologies

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e29909 - e29909

Published: April 21, 2024

According to information from the World Health Organization, world has experienced about 430 million cases of COVID-19, a world-wide health crisis caused by SARS-CoV-2 virus. This outbreak, originating China in 2019, led nearly 6 deaths worldwide. As number confirmed infections continues rise, need for cutting-edge techniques that can detect early and accurately become more critical. To address this, Federal Drug Administration (FDA) issued emergency use authorizations (EUAs) wide range diagnostic tools. These include tests based on detecting nucleic acids antigen-antibody reactions. The quantitative real-time reverse transcription PCR (qRT-PCR) assay stands out as gold standard virus detection. However, despite its accuracy, qRT-PCR limitations, such complex testing protocols risk false negatives, which drive continuous improvement acid serological approaches. emergence highly contagious variants coronavirus, Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529), increased specifically identify these mutations. article explores both acid-based assays, assessing performance recently approved FDA those documented scientific research, especially identifying new coronavirus strains.

Language: Английский

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Peptide Multifunctionalization via Modular Construction of Trans‐AB₂C Porphyrin on Resin

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract Peptide multifunctionalization is a crucial technique to develop peptide‐based agents for various purposes. Porphyrin‐peptide conjugates are class of popular multifunctional peptides renowned their and multimodal properties. However, the tedious synthetic works porphyrin building blocks involved in most previous studies. In this work, modular solid‐phase approach reported construct trans ‐AB 2 C on peptide chains without presynthesized blocks. The products from approach, which inherit both functionalities porphyrins modules employed constructing porphyrins, show potential biomedical biomaterial applications. Furthermore, by extending first example “resin‐to‐resin” reaction link two together along construction motifs give porphyrin‐peptide with different chains.

Language: Английский

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