Phytotherapy Research,
Journal Year:
2024,
Volume and Issue:
38(7), P. 3401 - 3416
Published: April 26, 2024
Abstract
Our
previous
research
confirmed
that
rutin
reduced
ventilator‐induced
lung
injury
(VILI)
in
mice.
Ferroptosis
has
been
reported
to
participate
the
pathogenic
process
of
VILI.
We
will
explore
whether
inhibits
ferroptosis
alleviate
A
mouse
model
VILI
was
constructed
with
or
without
pretreatment
perform
a
multiomics
analysis.
Hematoxylin–eosin
(HE)
staining
and
transmission
electron
microscopy
were
used
evaluate
Dihydroethidium
(DHE)
malondialdehyde
(MDA)
superoxide
dismutase
(SOD)
levels
detected.
Molecular
docking
performed
determine
binding
affinity
between
ferroptosis‐related
proteins.
Western
blot
analysis,
real‐time
PCR
(RT‐PCR)
immunohistochemical
(IHC)
conducted
detect
expression
GPX4,
XCT,
ACSL4,
FTH1,
AKT
p‐AKT
tissues.
Microscale
thermophoresis
(MST)
AKT1.
Transcriptomic
proteomic
analyses
showed
may
play
key
role
Metabolomic
analysis
demonstrated
affect
via
pathway.
indicated
regulate
Moreover,
upregulated
GPX4
downregulated
ACSL4
FTH1
Rutin
also
increased
ratio
p‐AKT/AKT
expression.
MST
binds
activate
signaling
pathway,
contributing
inhibit
ferroptosis,
thus
preventing
study
elucidated
possible
novel
strategy
involving
use
for
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(31), P. 11785 - 11792
Published: July 7, 2023
The
research
on
lipid
droplets
(LDs)
has
attracted
great
attention
in
the
field
of
biomedical
science
recent
years.
LD
malfunction
is
found
to
be
associated
with
development
acute
kidney
injury
(AKI).
To
monitor
this
biological
process
and
explain
related
pathological
behavior,
excellent
fluorescent
probes
a
polarity-sensitive
character
would
provide
desirable
strategy.
Herein,
we
designed
new
polarity-susceptible
probe
named
LD-B
targetability,
which
exhibits
very
weak
fluorescence
highly
polar
solvents
based
twisted
intramolecular
charge
transfer
effect
but
enhanced
low
environments,
enabling
us
visualize
polarity
alteration.
also
possesses
merits
intense
near-infrared
(NIR)
emission,
good
photostability,
large
Stokes
shift,
toxicity,
faster
metabolic
rate,
wash-free
ability;
thereby,
it
contribute
efficient
visualization
application.
Using
via
confocal
laser
scanning
imaging
small-animal
system
vivo,
first
manifested
prominent
rise
contrast-induced
AKI
(CI-AKI),
not
only
at
cellular
level
animals
vivo.
Furthermore,
vivo
studies
suggest
that
could
accumulate
kidney.
In
addition,
normal
cell
lines
(including
cells)
exhibiting
greater
LDs
than
cancer
cells
have
been
demonstrated
systemically.
Altogether,
our
work
presents
an
effective
approach
for
medical
diagnosis
CI-AKI
identification
potential
therapeutic
markers.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(11), P. 4570 - 4579
Published: March 5, 2024
Ferroptosis,
as
a
new
form
of
regulated
cell
death,
is
implicated
in
various
physiological
and
pathological
processes.
Developing
single
probe
for
an
independent
analysis
multiple
analytes
related
to
ferroptosis
can
provide
more
accurate
information
simplify
the
detection
procedures,
but
it
faces
great
challenges.
In
this
work,
we
develop
fluorescent
simultaneous
GSH
through
ratiometric
fluorescence
response
microviscosity
via
lifetime
model.
Based
on
reversible
Michael
addition
reaction
between
unsaturated
C═C
bond,
responds
reversibly
with
variation
fast
time
(t1/2
=
4.7
s).
At
same
time,
sensitive
environmental
viscosity
by
changing
its
lifetimes.
The
was
applied
monitor
drug-induced
process
both
classical
Xc–/GSH/GPX4-
DHODH-mediated
defense
mechanisms.
We
hope
that
will
useful
molecular
tool
real-time
live-cell
imaging
dynamics,
which
benefit
unveiling
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Jan. 23, 2024
Glioma,
as
the
most
frequently
occurring
primary
malignancy
in
central
nervous
system,
significantly
impacts
patients'
quality
of
life
and
cognitive
abilities.
Ferroptosis,
a
newly
discovered
form
cell
death,
is
characterized
by
significant
iron
accumulation
lipid
peroxidation.
This
process
fundamentally
dependent
on
iron.
Various
factors
inducing
ferroptosis
can
either
directly
or
indirectly
influence
glutathione
peroxidase,
leading
to
reduced
antioxidant
capabilities
an
increase
reactive
oxygen
species
(ROS)
within
cells,
culminating
oxidative
death.
Recent
research
indicates
strong
connection
between
range
pathophysiological
conditions,
including
tumors,
neurological
disorders,
ischemia-reperfusion
injuries,
kidney
damage,
hematological
diseases.
The
regulation
intervene
progression
these
diseases
has
emerged
major
area
interest
etiological
therapy.
However,
exact
functional
alterations
molecular
mechanisms
underlying
remain
be
extensively
studied.
review
firstly
explores
intricate
relationship
glioma,
highlighting
how
contributes
glioma
pathogenesis
cells
may
resist
this
Then,
we
discuss
recent
studies
that
have
identified
potential
inducers
inhibitors,
which
could
serve
novel
therapeutic
strategies
for
glioma.
We
also
examine
current
challenges
targeting
treatment,
complexity
its
need
precise
delivery
methods.
aims
provide
comprehensive
overview
state
offering
insights
into
future
broader
implications
death
pathway
cancer
biology.
