Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(31), P. 11723 - 11731
Published: July 26, 2023
Here,
a
novel
rapid
and
ultrasensitive
aptamer
biosensor
was
designed
for
target-induced
activation
of
AIE
effect
followed
by
the
Crispr
Cas12a
(LbCpf1)-mediated
cleavage
to
achieve
dual-signal
detection.
The
prepared
DNA
building
blocks
contain
target
aptamer,
ssDNA-Fc,
Activator1.
In
this
system,
mode
divided
into
two
steps.
First,
when
interacts
with
aptamers,
would
be
disintegrated
rapidly,
releasing
mass
Ac1,
generating
ETTC-dsDNA
aggregated
produce
fluorescence
signal
effect.
Second,
release
Ac2,
LbCpf1-crRNA
activated,
which
greatly
improves
ssDNA-Fc
efficiency
render
amplification
detection
target.
Satisfactorily,
using
approach
detect
gliotoxin,
optimal
conditions
achieved
reducing
time
55
min,
achieving
low
limit
2.4
fM
satisfactory
linear
in
range
50
1
nM,
addressed
shortcoming
weak
electrochemical
previous
sensors.
water-insoluble
material
coupled
obtain
water-soluble
successfully
introduced
sensor
5.6
fM.
Subsequently,
combined
handheld
workstation
applied
gliotoxin
five
actual
samples,
32.0
2.09
×
108
pM.
This
strategy
not
only
provides
effective
platform
mycotoxins
complex
food
matrices
but
also
opens
promising
avenue
various
molecules
imaging
disease
diagnosis.
