Analytical Sciences,
Journal Year:
2024,
Volume and Issue:
40(4), P. 765 - 772
Published: Feb. 15, 2024
As
one
of
the
most
fundamental
thiol
compounds
in
human
body,
cysteine
(Cys)
is
involved
maintaining
redox
balance.
Abnormal
Cys
levels
can
lead
to
various
diseases.
In
this
work,
we
successfully
synthesized
a
fluorescent
probe
(CTBA)
that
specifically
detect
using
acrylate
as
reaction
site,
and
CTBA
has
met
selectivity
anti-interference
for
detection
under
optimized
conditions.
The
linear
range
between
0.05
100
μM
limit
0.0381
μM.
Finally,
used
content
three
bovine
serum
samples
test
results
are
satisfactory.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(10), P. 3882 - 3891
Published: Sept. 22, 2023
Diabetic
cataract
(DC)
surgery
carries
risks
such
as
slow
wound
healing,
macular
edema,
and
progression
of
retinopathy
is
faced
with
a
deficiency
effective
drugs.
In
this
context,
we
proposed
protocol
to
evaluate
the
drug's
efficacy
using
lipid
droplets
(LDs)
marker.
For
purpose,
fluorescent
probe
PTZ-LD
for
LDs
detection
developed
based
on
phenothiazine
unit.
The
displays
polarity-dependent
emission
variations,
i.e.,
lower
polarity
leading
stronger
intensity.
Especially,
exhibits
photostability
superior
that
Nile
Red,
commercial
staining
dye.
Using
probe,
formation
in
DC-modeled
human
lens
epithelial
(HLE)
cells
validated,
interplay
LDs-LDs
LDs-others
are
investigated.
Unexpectedly,
transfer
between
visualized.
Moreover,
therapeutic
various
drugs
HLE
assessed.
Ultimately,
more
were
found
tissues
from
DC
patients
than
first
time.
We
anticipate
work
can
attract
attention
important
roles
during
progression.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(18), P. 7138 - 7144
Published: April 27, 2024
Superoxide
anion
(O2·–)
and
peroxynitrite
(ONOO–),
two
important
oxidants
under
oxidative
stress,
coexist
in
complex
cell
organism
systems,
playing
crucial
roles
various
physiological
pathological
processes,
particularly
neurodegenerative
diseases.
Despite
the
absence
of
robust
molecular
tools
capable
simultaneously
visualizing
O2·–
ONOO–
biosystems,
relationship
between
these
species
remains
understudied.
Herein,
we
present
sequentially
activated
fluorescent
probe,
DHX-SP,
which
exhibits
exceptional
selectivity
sensitivity
toward
ONOO–.
This
probe
enables
precise
imaging
living
PC12
cells
stress
conditions
using
distinct
fluorescence
signal
combinations.
Furthermore,
DHX-SP
has
ability
to
visualize
changes
levels
during
ferroptosis
Parkinson's
disease
model.
These
findings
establish
a
connection
crosstalk
phosphorus
group
stress.
Analytical Sciences,
Journal Year:
2024,
Volume and Issue:
40(4), P. 765 - 772
Published: Feb. 15, 2024
As
one
of
the
most
fundamental
thiol
compounds
in
human
body,
cysteine
(Cys)
is
involved
maintaining
redox
balance.
Abnormal
Cys
levels
can
lead
to
various
diseases.
In
this
work,
we
successfully
synthesized
a
fluorescent
probe
(CTBA)
that
specifically
detect
using
acrylate
as
reaction
site,
and
CTBA
has
met
selectivity
anti-interference
for
detection
under
optimized
conditions.
The
linear
range
between
0.05
100
μM
limit
0.0381
μM.
Finally,
used
content
three
bovine
serum
samples
test
results
are
satisfactory.
