Fully integrated and automated centrifugal microfluidic chip for point-of-care multiplexed molecular diagnostics

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 255, P. 116240 - 116240

Published: March 28, 2024

Language: Английский

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Revolutionary Point‐of‐Care Wearable Diagnostics for Early Disease Detection and Biomarker Discovery through Intelligent Technologies

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Early-stage disease detection, particularly in Point-Of-Care (POC) wearable formats, assumes pivotal role advancing healthcare services and precision-medicine. Public benefits of early detection extend beyond cost-effectively promoting outcomes, to also include reducing the risk comorbid diseases. Technological advancements enabling POC biomarker recognition empower discovery new markers for various health conditions. Integration wearables with intelligent frameworks represents ground-breaking innovations automation operations, conducting advanced large-scale data analysis, generating predictive models, facilitating remote guided clinical decision-making. These substantially alleviate socioeconomic burdens, creating a paradigm shift diagnostics, revolutionizing medical assessments technology development. This review explores critical topics recent progress development 1) systems solutions physiological monitoring, as well 2) discussing current trends adoption smart technologies within settings developing biological assays, ultimately 3) exploring utilities platforms discovery. Additionally, translation from research labs broader applications. It addresses associated risks, biases, challenges widespread Artificial Intelligence (AI) integration diagnostics systems, while systematically outlining potential prospects, challenges, opportunities.

Language: Английский

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Microfluidics-Assembled Nanovesicles for Nucleic Acid Delivery

Accounts of Chemical Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

ConspectusMicrofluidic technologies have become a highly effective platform for the precise and reproducible production of nanovesicles used in drug nucleic acid delivery. One their key advantages lies one-step assembly multidrug delivery nanovesicles, which improves batch-to-batch reproducibility by minimizing intermediate steps typically required conventional methods. These often involve complex hydrophobic electrostatic interactions, leading to variability nanovesicle composition performance. Microfluidic systems streamline encapsulation diverse therapeutic agents, including hydrophilic acids, proteins, both small molecules, within single chip, ensuring more consistent process. This capability enables codelivery multiple drugs targeting different disease pathways, is particularly valuable reducing risk resistance.Despite promise delivery, clinical translation has been hindered safety concerns, cytotoxicity, overshadowed efforts improve vivo stability efficiency. Positively charged commonly encapsulate negatively tend exhibit significant cytotoxicity. To address this, charge-shifting materials that respond pH changes or surface modifications proposed as promising strategies. Shifting charge from positive neutral negative at physiological can reduce enhancing feasibility these nanovesicle-based therapies.Microfluidic platforms offer control over properties, particle size, rigidity, morphology, Particle size relatively easy adjust controlling flow rates microfluidic channels, with higher generally producing smaller particles. However, continuous tuning rigidity remains challenging. By manipulation interfacial water layer between amphiphilic components during nanoparticle formation, future designs may achieve greater critical improving cellular uptake biodistribution. While shape using chips not yet fully explored biomedical applications, advances science enable this aspect future, offering further customization properties.The integration modification presents challenges due differing speeds processes. Nanovesicle rapid, whereas modifications, such those involving functional biomolecules, occur slowly require purification steps. Recent advances, rotary valve single-axis camshaft mechanisms, mixing stages process, allowing automation modification, thereby reproducibility.In conclusion, represent approach development multifunctional potential precision medicine. obstacles related scalability, remain, innovations chip design, materials, are paving way broader application settings. Future research, potentially incorporating machine learning, could optimize relationship properties biological outcomes, advancing use

Language: Английский

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Nanomaterial-mediated self-calibrating biosensors for ultra-precise detection of food hazards: Recent advances and new horizons

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 522, P. 216204 - 216204

Published: Sept. 10, 2024

Language: Английский

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Magnetofluidic-Assisted Portable Automated Microfluidic Devices for Protein Detection

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

To facilitate on-site detection by nonspecialists, there is a demand for the development of portable "sample-to-answer" devices capable executing all procedures in an automated or easy-to-operate manner. Here, we developed device that integrated magnetofluidic manipulation system and signal acquisition system. Both systems were controllable via smartphone. In device, mixing solutions magnetic beads static chamber was enhanced steel bead agitation, which improved reaction efficiency. We demonstrate performance using myoglobin as example. During process, plasma separated from whole blood sample homemade mini-centrifuge, subsequently, plasma, beads, reagents added to chip with multiple chambers. After loaded, initiated smartphone App Bluetooth. Then, shuttled through different chambers steps completed automatically: first, targets enriched antibody-modified followed washing, binding aptamer-functionalized G-quadruplex, amplifying (optional), chromogenic reaction. Finally, images colored captured processed obtain concentrations myoglobin. The limits depended on mode conversion, 0.1 2.7 nM (with without amplifying). With its simple operation, compact design, low cost, ease scalability, this holds potential applications human health, food safety, environmental monitoring, etc.

Language: Английский

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An amplification-free digital droplet assay for influenza A viral RNA based on CRISPR/Cas13a

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Scheme of the amplification-free digital droplet assay. Two innovations: (1) polystyrene capture beads to concentrate target RNA from a dilute sample and (2) “confinement” effect amplifies concentration molecule.

Language: Английский

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Logic-Measurer: A Multienzyme-Assisted Ultrasensitive Circuit for Logical Detection of Exosomal MicroRNAs

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

The logic profiling of exosomal microRNAs (miRNAs) offers broad potential applications in the accurate diagnosis and staging cancer. However, logical detection low-abundance miRNAs complex clinical samples remains challenging. This study introduces a analysis system termed "Measurer" (a multi-enzyme-assisted ultrasensitive circuit) that versatile method for detecting multiple miRNAs. Logic-Measurer comprises three modules: stem-loop hairpin-enhanced CRISPR/Cas13a, polymerase-driven primer exchange reaction, an exonuclease III-mediated fluorescence output. efficient was switched by faster rate trans-cleavage activity Cas13a due to its improved affinity hairpin RNA structures. mechanistic model CRISPR/Cas13a confirmed molecular dynamics simulations. accurately detected miRNA-21 or miRNA-375 down 2.1 4.4 fM, with superior specificity, enabled situ as low 1.4 × 102 particles/mL exosomes via membrane fusion. In addition, this demonstrated 87.3 82.1% accuracy early breast cancer, respectively, among cohort 315 individuals. Subsequent subgroup further method's ability differentiate estrogen receptor-positive patients from healthy Therefore, valuable insights into development CRISPR/Cas-based enhanced diagnostic platform next generation technology based on enzyme circuits.

Language: Английский

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Temperature-programmed microfluidic CRISPR diagnostics enable rapid and automatous point-of-care testing for syphilis

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154174 - 154174

Published: July 21, 2024

Language: Английский

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Rapid detection of mexX in Pseudomonas aeruginosa based on CRISPR-Cas13a coupled with recombinase polymerase amplification

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 31, 2024

The principal pathogen responsible for chronic urinary tract infections, immunocompromised hosts, and cystic fibrosis patients is Pseudomonas aeruginosa , which difficult to eradicate. Due the extensive use of antibiotics, multidrug-resistant P. has evolved, complicating clinical therapy. Therefore, a rapid efficient approach detecting strains their resistance genes necessary early diagnosis appropriate treatment. This study combines recombinase polymerase amplification (RPA) clustered regularly interspaced short palindromic repeats-association protein 13a (CRISPR-Cas13a) establish one-tube two-step reaction systems mexX gene in . test times RPA-Cas13a methods were 5 40 min (including 30 RPA reaction), respectively. Both outperform Quantitative Real-time Polymerase Chain Reactions (qRT-PCR) traditional PCR. limit detection (LoD) genome 10 aM 1 aM, Meanwhile, designed primers have high specificity gene. These two also verified with actual samples isolated from industrial settings demonstrated great accuracy. Furthermore, results assay could be visualized using commercial lateral flow dipstick LoD fM, useful adjunt gold-standard qRT-PCR field detection. Taken together, procedure developed this CRISPR-Cas13a provides simple fast way genes.

Language: Английский

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Exonuclease III/Cas12a Cascade Amplification Strategy and Smartphone-Based Portable Fluorescence Detector to Repurpose the Commercial AFP Strip for the POCT of Multiple RNAs

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(32), P. 13252 - 13259

Published: July 31, 2024

Point of care testing (POCT) nucleic acid (NA) contributes to the timely disease diagnosis, like bacteria and virus screening in households or resource-constrained areas, but its development has always been stagnant. Herein, we proposed an exonuclease III cascaded with CRISPR/Cas12a (Exo-III/Cas12a) amplification strategy constructed a smartphone-based portable fluorescence detector (SPFD) repurpose commercial alpha-fetoprotein (AFP) strip for ultrasensitive hand-held detection NA samples. In detail, target-initiated-Exo-III/Cas12a realizes signal liberates AFP from magnetic beads through trans-cleavages activated Cas12a toward aptamer. After separation migration, signals test (FT) control (FC) lines on were digitally output by SPFD, FT/FC was employed quantitative analysis minimize external disturbances improve accuracy. We experimentally assessed universe applicability NA-POCT platform miRNA-155, 16S rRNA Staphylococcus aureus, ORF1a/b RNA Covid-19 pseudovirus, achieving favorable limits 42 aM, 18 CFU/mL, 87 copies/μL, respectively. Moreover, simplicity, universality, admirable performance demonstrate great potential aspect rapidly transforming existing POCT devices multiple new applications at time need.

Language: Английский

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