Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 26, 2024
A
simple,
rapid,
and
visual
approach
is
developed
to
perform
diagnosis
of
urinary
tract
infection
(UTI)
antimicrobial
susceptibility
testing
(AST)
by
employing
smart
bifunctional
DNA
(bfDNA)
sensors,
exonuclease
III,
concatermers
CuO
nanoparticles
(CuONPs),
gold
NPs
(AuNPs)
aggregation
[AuNPs
agglutination
(AA)],
namely,
the
bfDEC-AA
method.
The
bfDNA
sensors
serve
as
probes
for
identifying
16S
rRNA
genes
bacterium
or
18S
fungus
mediators
connecting
CuONPs.
AA
a
signal
source
triggered
Cu(I)-catalyzed
azide–alkyne
cycloaddition
click
chemistry.
In
this
study,
urine
samples
are
analyzed
directly,
eliminating
need
overnight
incubation
bacterial
isolation.
crucial
color
change
from
red
purple
occurs
at
UTI
diagnostic
threshold
104
CFU
mL–1;
thus
results
can
be
qualitatively
interpreted
unaided
eyes.
Similarly,
AST
was
performed
visually
under
our
optimal
conditions.
shift
also
quantified
using
point-of-care
(POC)
device.
identification,
bacteria
strain
were
achieved
within
55,
145
min
96
samples,
respectively.
Here,
128
suspected
patients
tested.
Notably,
sensitivity
specificity
method
99%
100%
Gram-negative
(G–)
infection,
Gram-positive
(G+)
98%
AST,
Furthermore,
low
cost
POC
device
(US$156)
friendly
underdeveloped
regions.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
2(1)
Published: Jan. 1, 2024
Abstract
Paper‐based
devices
have
attracted
considerable
attention
in
point‐of‐care
testing
(POCT)
due
to
their
simple
operation
and
portability.
The
performance
of
paper‐based
POC
assays
is
further
enhanced
by
coupling
with
functional
nucleic
acids
(FNAs),
which
are
able
selectively
recognize
bind
targets,
amplify
transduce
signals.
Several
high‐performance
been
developed,
resulting
from
the
different
spatial
structures
device
detection
strategies
using
FNAs.
In
this
review,
we
first
introduce
FNAs
devices,
including
concepts,
classifications
advances.
following
section
focuses
on
application
these
taking
into
account
one‐,
two‐
three‐dimensional
structures.
Finally,
challenges
perspectives
POCT
discussed.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(2), P. 68 - 68
Published: Jan. 23, 2025
Multiplex
lateral
flow
assays
are
one
of
the
greatest
advancements
in
world
rapid
diagnostics,
achieving
performance
several
tests
one.
These
meet
basic
requirements
increasing
ease
use,
low
detection
limit,
and
high
specificity,
as
they
combine
use
novel
strategies,
such
exploitation
multiple
labels,
a
variety
amplification
methods.
have
proven
their
usefulness
many
different
areas,
including
clinical
food,
environmental
monitoring.
In
this
review
paper,
we
attempt
to
highlight
discuss
predominant
changes
multianalyte
LFAs,
related
principle,
development,
combination
with
other
Attention
is
paid
flexibility
challenges
associated
LFA
arrays,
strategies
improve
detectability,
sensitivity,
reliability
assays.
Therefore,
emphasizes
current
advances
field
underline
possible
impact
multiplex
LFAs
on
future
diagnostics
analytical
sciences.
