New Frontiers in CRISPR: Addressing Antimicrobial Resistance with Cas9, Cas12, Cas13, and Cas14

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e42013 - e42013

Published: Jan. 1, 2025

The issue of antimicrobial resistance (AMR) poses a major challenge to global health, evidenced by alarming mortality predictions and the diminishing efficiency conventional drugs. CRISPR-Cas system has proven be powerful tool in addressing this challenge. It originated from bacterial adaptive immune mechanisms gained significant recognition scientific community. This review aims explore applications technologies combating AMR, evaluating their effectiveness, challenges, potential for integration into current strategies. We conducted comprehensive recent literature databases such as PubMed Web Science, focusing on studies that employ against AMR. offer transformative approach combat with extend beyond traditional Integrating these existing methods could significantly enhance our ability manage potentially reverse growing problem resistance. Future research should address technical ethical barriers facilitate safe effective clinical environmental applications. underscores necessity interdisciplinary collaboration international cooperation harness full fight superbugs.

Language: Английский

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Dual-OR Logic-Gated Lateral Flow Strip Assay Based on Colorimetric-Fluorescence Dual Indication for Screening of HPV16/18 in Multiple Scenarios

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

The incidence of cervical cancer continues to rise in underdeveloped regions due low human papillomavirus (HPV) vaccination rates and inadequate screening systems. To achieve convenient, rapid, accurate detection HPV, we developed a three-wire lateral flow strip assay system based on dual-OR logic gates for rapid simultaneous HPV subtypes 16 18 single test. combines three-branch-catalytic hairpin assembly (TCHA)-mediated signal amplification with simple OR gate-based output improve while enabling 16/18 subtype identification. limit the method was calculated be 10 aM selected target sequences. Meanwhile, showed excellent specificity no false-positive real-world detection. sensitivity colorimetric test strips exceeded 90%, fluorescence-based surpassed 95% detecting clinical samples, demonstrating high degree concordance results obtained from real-time quantitative polymerase chain reaction (qPCR). This provides easy HPV.

Language: Английский

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Fluorescence detection of the genetic risk factor ApoE4 gene associate with Alzheimer’s disease based on a CRISPR-Cas12a system

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112831 - 112831

Published: Jan. 1, 2025

Language: Английский

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Fast-Responsive HClO-Activated Near-Infrared Fluorescent Probe for In Vivo Diagnosis of Inflammatory Bowel Disease and Ex Vivo Optical Fecal Analysis

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(29), P. 12065 - 12073

Published: July 10, 2024

Inflammatory bowel disease (IBD) is an idiopathic intestinal inflammatory disease, whose etiology intimately related to the overproduction of hypochlorous acid (HClO). Optical monitoring HClO in living body favors real-time diagnosis diseases. However, HClO-activated near-infrared (NIR) fluorescent probes with rapid response and high cell uptake are still lacking. Herein, we report activatable acceptor-π-acceptor (A-π-A)-type NIR probe (

Language: Английский

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Engineering Acid-Promoted Two-Photon Ratiometric Nanoprobes for Evaluating HClO in Lysosomes and Inflammatory Bowel Disease

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 11, 2025

HClO is considered a potential contributing factor and biomarker of inflammatory bowel disease (IBD). Accurate monitoring lysosomal important for further developing specific diagnostic therapeutic schedules IBD. However, only rare types fluorescent probes have been reported detecting in IBD so far. Herein, an acid-promoted two-photon semiconducting polymer dot (Lyso-RS Pdot) with dual emission green red channels dual-sensing sites successfully fabricated two newly designed polymers NADE-PSMA PFNA-10TBT as precursors. The conjugated pH-inert HClO-sensitive units employed to evaluate the concentration turn-off fluorescence. Meanwhile, amphiphilic sensitive pH value or turn-on resultant Lyso-RS Pdots not display satisfactory performances but also achieve accurate imaging lysosomes colon mice based on distinguished properties such ratiometric signal output, amplification, ultrafast response, excitation. results demonstrate that level elevated, fast early diagnosis can be achieved through fluorescence by proposed Pdots. This work may provide some solid perspectives H

Language: Английский

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Detection of Chloramphenicol by nucleic acid cyclic amplification fluorescence analysis and lateral flow assay assisted with CRISPR/Cas12a and Exonuclease III

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106477 - 106477

Published: March 1, 2025

Language: Английский

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BE-CATCH: Bioamplifier-Equipped CRISPR-Cas12a Transduction System Coupled with Commercial Pregnancy Test Strips to Harness Signal-on Point-of-Care Detection

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Repurposing existing commercial diagnostic equipment to enable portable analysis of diverse targets is driving the development affordable point-of-care testing (POCT). Interestingly, we found that goat antimouse IgG could replace human chorionic gonadotropin (hCG) make T line pregnancy test strips (PTS) appear red color and accordingly synthesized a novel signal output probe, which eliminated intricate hCG covalent coupling steps, meet multiple needs expanded POCT. Given this, introduced separation-free universal POCT strategy termed bioamplifier-equipped CRISPR-Cas12a transduction system coupled with PTS harness signal-on detection (BE-CATCH). Specifically, target inputs were converted amplified by multiplied strand displacement amplification-based bioamplifier, thereby activating Cas12a's trans-cleavage activity. Then, activated Cas12a would cleave connector indiscriminately, ultimately kept probe in free state; thus, be translated into colorimetric on PTS. This not only provided boosted sensitivity specificity but also enhanced user-friendliness maintaining mode. We demonstrated versatility BE-CATCH through selectively detecting miR-155 flap endonuclease 1. its broad adaptability, provide an appealing option broaden application biomedical diagnostics.

Language: Английский

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Recent advances in the CRISPR/Cas system-based visual detection method

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Reviews the development of visual detection methods in recent years based on different Cas and analyzes their advantages disadvantages as well challenges future research.

Language: Английский

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Refining nanoprobes for monitoring of inflammatory bowel disease

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 177, P. 37 - 49

Published: Feb. 15, 2024

Language: Английский

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Commercial Strip‐Inspired One‐Pot CRISPR‐Based Chip for Multiplexed Detection of Respiratory Viruses

Small Methods, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 19, 2024

The absence of sensitive, multiplexed, and point-of-care assays poses a critical obstacle in promptly responding to emerging human respiratory virus (HRV) pandemics. Herein, RECOGNIZER (re-building commercial pregnancy strips via large-size nanoflowers), an innovative one-pot CRISPR assay, is presented that employs commercially available identify several types HRVs. superiority the assay mainly relies on two aspects: (i) DNA nanoflowers possessing high surface-to-volume ratio well-defined surface allow for considerable probe loading density minimized non-specific interaction, achieving impressive signal-to-noise proportion exceeding tenfold at 1 nM target. (ii) design reaction, multi-channel chip, custom-made app enables rapid, sample-to-answer, multiplexed analysis four HRVs 25 min. This demonstrates sensitivity 5.42 pM synthetic SARS-CoV-2 RNA 10 copies µL-1 plasmids after pre-amplification. Finally, proposed approach indicated 100% accuracy 50 clinical swab samples, demonstrating robust performance distinguishing from other versatility scalability renders it user-friendly platform infection monitoring, offering significant potential improving pandemic response efforts.

Language: Английский

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