Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 423, P. 136836 - 136836
Published: Oct. 23, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)
Published: June 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Language: Английский
Citations
35
Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 110990 - 110990
Published: Feb. 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Extracellular vesicles (EVs) play crucial roles in intercellular communication and hold great promise as biomarkers for noninvasive disease diagnosis. Intensive research efforts have been devoted to discovering the EV subpopulations responsible specific functions or with enhanced effectiveness markers, through extensive purification content analysis. However, their high heterogeneity size cargo composition poses significant challenges reaching such goals. Isolation methods like ultracentrifugation size-exclusion chromatography, well analysis approaches polymerase chain reaction enzyme-linked immunosorbent assay, made contributions improving our understanding of biology. Nonetheless, these face limitations isolation efficiency, purity, detection sensitivity specificity due issues large sample consumption, unsatisfactory insufficient resolution subtyping. Microfluidic technology presents promising solutions challenges, leveraging intrinsic capabilities precise flow external energy field manipulation, compartmentalization, signal enhancement at micro- nanoscale. Hence, this review summarizes recent developments microfluidics-enabled analysis, paying special attention unique microfluidic features exploited. Strategies viscoelastic inertial flow, fluid mixing, external-field-assisted purification, compartmentalization micro/nanostructures enhancing detection, are examined. Furthermore, current potential future directions discussed inspire advancements rapidly developing field.
Language: Английский
Citations
0
Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 586 - 586
Published: Aug. 2, 2024
Brain tumors, and, in particular, glioblastoma (GBM), are among the most aggressive forms of cancer. In spite advancement available therapies, both diagnosis and treatments still unable to ensure pathology-free survival GBM patients for more than 12–15 months. At basis poor ability cope with brain we can consider: (i) intra-tumor heterogeneity; (ii) heterogeneity tumor properties when compare different patients; (iii) blood–brain barrier (BBB), which makes difficult isolation tumor-specific biomarkers delivering therapeutic drugs brain. Recently, it is becoming increasingly clear that cancer cells release large amounts extracellular vesicles (EVs) transport metabolites, proteins, classes RNAs, DNA, lipids. These structures involved pathological process characterize any particular form Moreover, EVs able cross BBB directions. Starting from these observations, researchers now evaluating possibility use purified organic fluids (first all, blood saliva), order obtain, through non-invasive methods (liquid biopsy), biomarkers, perhaps, also obtaining nanocarriers targeted drugs.
Language: Английский
Citations
3
Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)
Published: Oct. 14, 2024
Cell-derived extracellular vesicles (EVs), which carry diverse biomolecules such as nucleic acids, proteins, metabolites, and lipids reflecting their cell of origin, are released under both physiological pathological conditions. EVs have been demonstrated to mediate cell-to-cell communication serve biomarkers. EV-associated DNA (EV-DNA) comprises genomic mitochondrial (i.e., gDNA mtDNA) fragments. Some studies revealed that EV-DNA can represent the full nuclear genome parental cells. Furthermore, fragments loaded into stable be transferred recipient cells regulate biological functions. In this review, we summarized discussed research advances with an emphasis on detection at population-EV single-EV levels, gene transfer-associated functions, clinical applications biomarkers for disease liquid biopsy. We hope review will provide potential directions or guidance future investigations.
Language: Английский
Citations
2
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 423, P. 136836 - 136836
Published: Oct. 23, 2024
Language: Английский
Citations
1