Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

The year 2024 marks the 50th anniversary of discovery surface-enhanced Raman spectroscopy (SERS). Over recent years, SERS has experienced rapid development and became a critical tool in biomedicine with its unparalleled sensitivity molecular specificity. This review summarizes advancements challenges substrates, nanotags, instrumentation, spectral analysis for biomedical applications. We highlight key developments colloidal solid an emphasis on surface chemistry, hotspot design, 3D hydrogel plasmonic architectures. Additionally, we introduce innovations including those interior gaps, orthogonal reporters, near-infrared-II-responsive properties, along biomimetic coatings. Emerging technologies such as optical tweezers, nanopores, wearable sensors have expanded capabilities single-cell single-molecule analysis. Advances analysis, signal digitalization, denoising, deep learning algorithms, improved quantification complex biological data. Finally, this discusses applications nucleic acid detection, protein characterization, metabolite monitoring, vivo spectroscopy, emphasizing potential liquid biopsy, metabolic phenotyping, extracellular vesicle diagnostics. concludes perspective clinical translation SERS, addressing commercialization potentials tissue sensing imaging.

Language: Английский

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“Three-in-One” Plasmonic Au@PtOs Nanocluster Driven Lateral Flow Assay for Multimodal Cancer Exosome Biosensing

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10686 - 10695

Published: June 17, 2024

Exploiting the multiple properties of nanozymes for multimode lateral flow assay (LFA) is urgently required to improve accuracy and versatility. Herein, we developed a novel plasmonic Au nanostar@PtOs nanocluster (Au@PtOs) as signal tag LFA detection. Based on PtOs bimetallic doping strategy, Au@PtOs can indicate both excellent SERS enhancement nanozyme catalytic activity. Meanwhile, displays better photothermal effect than that nanostars. Therefore, colorimetric/SERS/temperature three-mode signals be read out based nanocomposite. The was combined with applied breast cancer exosome detection limit mode 2.6 × 103/4.1 101/4.6 102 exosomes/μL, respectively, which much superior common nanoparticles (∼105 exosomes/μL). Moreover, fingerprint molecular recognition ability mode, phenotypes derived from different cell lines discriminated easily. convenient visual colorimetric sensitive SERS/temperature quantitative modes, driven satisfy requirements accurate flexible multimodal sensing in application scenarios.

Language: Английский

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Nanozyme-Inhibited SERS Multichannel Paper-Based Sensor Array for the Quantification and Identification of Biothiols and Cancer Cells Based on Three Ag-Based Nanomaterials

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11353 - 11365

Published: July 6, 2024

Biothiols play essential roles in maintaining normal physiological functions, resisting oxidative stress, and protecting cell health. Establishing an effective reliable sensor array for the accurate quantification discrimination of diverse biothiols is extremely meaningful. In this work, Ag/Mn3O4, Ag3PO4, Ag3Cit with excellent oxidase-mimetic activity surface-enhanced Raman scattering (SERS)-enhanced features have been prepared loaded onto Whatman filter paper (WFP) to build SERS chips as three sensing channels, which can induce 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation SERS-active reporters (TMBox) concurrently generate prominent signals. Nevertheless, addition suppress conversion from TMB TMBox, cause reduction signal TMBox. Interestingly, each channel different TMBox signals' variations due differences inhibition abilities exclusive properties chip, be plotted specific fingerprint patterns biothiol further translated into intuitive two-dimensional (2D) clustering profiles through linear discriminant analysis (LDA) hierarchical cluster (HCA) techniques precise identification these six minimum concentration 1 μM. More importantly, exploited intracellular glutathione (GSH), differentiate between cancer cells based on GSH contents even identify types tumor cells, demonstrating its powerful application prospects disease diagnosis.

Language: Английский

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Bifunctional MoO_3–x/CuS Heterojunction Nanozyme-Driven “Turn-On” SERS Signal for the Sensitive Detection of Cerebral Infarction Biomarker S100B

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(44), P. 17711 - 17719

Published: Oct. 25, 2024

The use of nanozymes has become a promising auxiliary approach to "turn on" surface-enhanced Raman scattering (SERS) signals for the label-free detection disease markers. Nevertheless, there are still major challenges develop bifunctional nanomaterials with both excellent enzyme-like activity and high SERS performance. To this end, novel Z-scheme MoO

Language: Английский

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Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(51), P. 71048 - 71059

Published: Dec. 12, 2024

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method identify quantify various accurately. Inspired by iron porphyrin active center natural horseradish peroxidase (HRP), we designed synthesized two covalent organic frameworks (Fe-COF-H Fe-COF-OH) with notable peroxidase-like (POD) activity, capable catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB three distinct absorption peaks. Based on these, six-channel nanozyme colorimetric sensor array was constructed, which could map specific fingerprints thiols. Subsequently, machine learning techniques, including supervised linear discriminant analysis (LDA), decision trees (DT) artificial neural networks (ANN), unsupervised hierarchical cluster (HCA), ensemble random forests (RF), were used for precise identification complex systems, detection limit as low 50 nM. Significantly, demonstrated strong potential practical applications, analyzing homocysteine (Hcy) human serum, mercaptoacetic acid (TGA) depilatory creams, glutathione (GSH) cell lysates, thereby showing promise use disease diagnosis.

Language: Английский

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Cryogenically Induced Highly Ordered Single-Strand DNA-Modified Magnetic Nanoprobes for Rapid and Ultrasensitive Bioanalysis

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

The development of highly sensitive detection methods for bioanalysis is crucial early disease diagnosis. Electrochemical biosensing technology offers unique advantages in this area due to its rapid response, high sensitivity, and low cost. However, achieving efficient transfer signaling molecules the electrode interface facilitate effective interaction between signal sensing surface remains a critical challenge ultrasensitive electrochemical detection. In study, we discovered that single-stranded DNA-modified magnetic nanoprobes (signal probe A) subjected cryogenic treatment can rapidly form an orderly monolayer at under external field, while phenomenon was not observed with double-stranded B). Building on finding, developed protective complementary strand B) that, upon target molecules, converted into A. This transformation, combined treatment, enables molecules. Using miRNA-21 carcinoembryonic antigen (CEA) as model targets, optimized conditions, limit 3.4 aM 0.28 fg/mL CEA excellent versatility. summary, study introduces efficient, rapid, enzyme-free, environmentally friendly amplification strategy. approach only provides innovative solution but also new insights enhancing molecule–sensor interactions biosensors.

Language: Английский

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Recent Advances in Nanozyme Sensors Based on Metal–Organic Frameworks and Covalent–Organic Frameworks

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 520 - 520

Published: Oct. 23, 2024

Nanozymes are nanomaterials that exhibit enzyme-like catalytic activity, which have drawn increasing attention on account of their unique superiorities including very high robustness, low cost, and ease modification. Metal-organic frameworks (MOFs) covalent-organic (COFs) emerged as promising candidates for nanozymes due to abundant activity centers, inherent porosity, tunable chemical functionalities. In this review, we first compare the enzyme-mimicking centers mechanisms between MOF COF nanozymes, then summarize recent research designing modifying with activity. Moreover, typical examples sensing applications based these presented, well translation enzyme into a visible signal response. At last, discussion current challenges is followed by some future prospects provide guidance nanozyme sensors MOFs COFs practical applications.

Language: Английский

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Defect-Engineered CoFe Layered Double Hydroxide Quantum Dots: Oxygen Vacancy-Driven Boost in Peroxidase-Mimic Catalysis for Multiplex Biomarker Sensing

ACS Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1777 - 1785

Published: April 8, 2025

Language: Английский

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