Turning Waste into Wealth: Enzyme-Activated DNA Sensor Based on Reactant Recycle for Spatially Selective Imaging MicroRNA toward Target Cells DOI
Yaokun Xia,

Guomin Ju,

Yongting Zhou

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1337, P. 343557 - 343557

Published: Dec. 16, 2024

Language: Английский

Dual miRNA-Triggered DNA Walker Assisted by APE1 for Specific Recognition of Tumor Cells DOI

Qianying Zhou,

Tong Li, Xidong Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(17), P. 6774 - 6783

Published: April 18, 2024

The identification of a specific tumor cell is crucial for the early diagnosis and treatment cancer. However, it remains challenge due to limited sensitivity accuracy, long response time, low contrast recent approaches. In this study, we develop dual miRNA-triggered DNA walker (DMTDW) assisted by APE1 recognition cells. miR-10b miR-155 were selected as research models. Without presence, inactive its walking strand W locked L1 L2. After are input, triggered bind L2 W-L1-L2, respectively, unlocking W. driven endogenous that highly catalytic expressed in cytoplasm cells but barely normal cells, ensuring high reaction efficiency Dual miRNA input required trigger walker, making strategy with accuracy. DMTDW exhibited analysis detection limit 44.05 pM. Living cell-imaging experiments confirmed could effectively respond fluctuation specifically identified MDA-MB-231 from different lines. proposed sensitive, rapid, accurate recognition. We believe can become powerful diagnostic tool

Language: Английский

Citations

9
Highly integrated, self-powered and activatable bipedal DNA nanowalker for imaging of base excision repair in living cells DOI Creative Commons

Rongji Lai,

Xianhui Pan,

Yingfeng Qin

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 18, 2024

Language: Английский

Citations

5
High-Efficient Electrochemiluminescence of DNA-Au Ag Nanoclusters with Au NPs@Ti3C2 as a Novel Coreaction Accelerator for Ultrasensitive Biosensing DOI
Huiling Wu, Xiaochun Zhu, Xiaoyu Shi

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(33), P. 13727 - 13733

Published: Aug. 7, 2024

In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed based on DNA-stabilized Au Ag nanoclusters (DNA-Au NCs) as the efficient luminophore and NPs@Ti

Language: Английский

Citations

4
Enhancing early breast cancer detection with APE1-triggered oligonucleotide probes and graphene oxide: The impact of variable AP site modification on sensitivity and specificity DOI
Xiaoxiao Yu,

Sujian Cao,

Xuexian Deng

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127505 - 127505

Published: Jan. 24, 2025

Language: Английский

Citations

0
Engineering DNA circuit powered by entropy integrated into robust and elegant photoelectrochemical and photothermal dual-mode biosensing DOI
Jing Li,

Bingtao Hu,

Yanxin Zhang

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Citations

0
A dual-signal Amplification Strategy for Electrochemical Biosensing of Transfer RNA-derived Fragments Targeting Drug-Resistant Liver Cancer DOI

Xixiang Xie,

Chaofang Dong, Jiangtao Liu

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137693 - 137693

Published: March 1, 2025

Language: Английский

Citations

0
Target-Triggered Cascaded Self-Feedback DNAzyme Circuit Loaded in ZIF-8 for Highly Sensitive miRNA Imaging in Living Cells DOI
Ting Huang,

Nai-Han Huang,

Qianying Zhou

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

The highly sensitive and rapid imaging of miRNA in living cells promises to advance our understanding diseases promote their diagnosis treatment. To enhance the sensitivity imaging, a series cascade signal amplification strategies based on enzyme-free methods have been developed. However, these cascaded involve complex designs multiple mechanisms, leading potential side effects. Herein, we developed novel hairpins@ZIF-8 nanosystem by rationally integrating ZIF-8 with self-feedback DNAzyme circuit (CSDC), achieving cells. facilitates efficient transfection nucleic acid probes into provides necessary cofactor ions for CSDC. CSDC possesses exponential solely mechanism. Benefiting from efficiency, exhibited higher than traditional DNAzyme-based amplification, detection limit 2.28 fM, approximately 105 times more amplification. This demonstrated strong practical application capabilities, effectively reflecting fluctuations intracellular levels successfully distinguishing between normal tumor expression differences. It could also be applied vivo imaging. proposed strategy is anticipated pave way innovative approaches serve as vital instrument miRNA-related research, diagnosis,

Language: Английский

Citations

0
Lipidic Nanoclusters-Assisted Spatial Confinement Signal Amplification for Ratiometric MicroRNA Sensing DOI

Haiyan Jia,

Ningfang Wu,

Jiang Zhu

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
An innovative “double-locked” CRISPR/Cas12a system based on DNAzyme for the precise imaging of microRNAs in living cells DOI
Lu Lu, Si-Lin Gan,

Shixiu Xiao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143605 - 143605

Published: April 28, 2025

Language: Английский

Citations

0
Ingenious Entropy-Driven DNA Circuit Intercommunicating with DNAzyme-Powered DNA Walker for Dual-Mode Biosensing DOI
Jingling Zhang,

Defu Qian,

Qingqing Tan

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 136668 - 136668

Published: Sept. 1, 2024

Language: Английский

Citations

2