Molecules,
Journal Year:
2024,
Volume and Issue:
29(20), P. 4857 - 4857
Published: Oct. 13, 2024
Electrochemiluminescence
(ECL)
detection
is
widely
applied
in
many
fields,
including
chemical
measurement,
biological
analysis,
and
clinic
tests,
due
to
its
high
sensitivity.
Currently,
the
fast
development
of
new
electrochemical
luminophores
continuously
improving
ECL-based
ability.
Besides
enhancement
luminescence
emission
for
a
sensitivity,
minimizing
effect
co-reactants
on
ECL
achieving
multiple
analysis
one
sample
are
also
main
directions
this
field.
This
review
focuses
summary
recently
prepared
achieve
three
aims
mentioned
above.
Especially,
composed
by
parts,
focusing
or
materials
with
efficiency,
self-enhancing
properties,
multi-color
luminophores.
The
fabrication
biosensors
using
these
molecules
reviewed
exhibit
advances
applications.
Herein,
a
visual
electrochemiluminescence
(ECL)
luminophore,
6-azido-2-thioxanthine-coated
gold
nanoclusters
(ATT-Au
NCs),
was
prepared
efficiently
in
single
step,
followed
by
comprehensive
characterization
of
their
structural,
optical,
and
ECL
properties
using
diverse
analytical
methodologies.
Concurrently,
nanoparticles,
dimers,
nanorod
(Au
NR)
dispersions,
dimers
(parallel
perpendicular
conformations)
were
synthesized
via
chemical
reduction,
DNA
ligation,
seed
growth,
electrostatic
adsorption
organic
ligands,
respectively.
The
finite
difference
time
domain
(FDTD)
modeling
subsequently
employed
to
analyze
the
electromagnetic
field
distribution
surrounding
these
revealing
that
parallel
notably
enhanced
intensity.
Based
on
this,
we
constructed
novel
biosensor
harnessed
surface-plasmon-coupled
(SPC-ECL)
resonance
energy
transfer
(RET)
between
ATT-Au
NCs
Au
NR
dimers.
sensor
incorporated
Cu2O
nanoparticles
(NPs)
as
quenching
probes
precisely
induce
RET,
leading
signal
being
switched
"off".
This
dual
enhancement
strategy
achieved
high
signal-to-noise
ratio,
facilitating
sensitive
detection
microRNA-21
(miRNA-21)
with
linear
range
1
fM–100
nM
low
limit
0.28
fM.
work
not
only
extends
our
understanding
SPC
effect
application
RET
mechanism
ECL,
providing
theoretical
foundation
for
further
advancements
field,
but
also
highlights
its
considerable
potential
applications
biomedical
research
clinical
diagnostics.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(31), P. 12838 - 12845
Published: July 25, 2024
MicroRNA
(miRNA)
detection
is
a
critical
aspect
of
disease
diagnosis,
and
recent
studies
indicate
that
miRNA-622
could
be
potential
target
for
lung
cancer.
Herein,
Cu
single
atoms
were
anchored
on
graphitic
carbon
nitride
(Cu
SAs@CN)
as
coreaction
accelerator
applied
in
luminol-H
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 24, 2024
The
classical
electrochemiluminescence
(ECL)
reagent
Ru(bpy)32+
was
first
doped
into
CdSe
QDs
to
prepare
novel
dual-potential
color-resolved
luminophore
Ru-CdSe
QDs.
emitted
a
strong
red
ECL
signal
at
positive
potential
with
coreactant
TPrA
and
green
negative
K2S2O8.
As
proof-of-concept
application,
this
work
introduced
dual-channel
closed
bipolar
electrode
(CBPE)
system
construct
an
biosensor
for
simultaneous
detection
of
chloramphenicol
(CAP)
kanamycin
(KAN).
were
dropped
on
BPE
holes
A
B
emission.
Cobalt
single-atom
catalysts
(Co–N–C
SACs)
had
superior
electric
double
layer
(EDL)
performance
conductivity.
It
could
greatly
promote
the
electron
transfer
CBPE
realize
amplification.
Based
characteristic,
Co–N–C
SACs
hole
C
driving
D,
respectively,
using
CAP
KAN
split
dual-aptamer
sandwich
strategy.
During
scanning,
polarity
anode,
signal.
With
increase
concentration,
abundant
surface.
increased
detection.
cathode,
Finally,
zero-background
spatial-potential
CBPE–ECL
developed
dual-mode
KAN.
This
explored
sensor,
which
facilitated
development
assay.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 6, 2025
Traditional
electrochemiluminescence
(ECL)
detection
makes
it
difficult
to
realize
the
spatial
separation
of
sensing
and
reporting
sides,
which
inevitably
causes
mutual
interference
between
target
luminescent
substance.
By
studying
relationship
luminol
luminescence
position
electrode
potential
in
a
three-electrode
system,
this
work
realized
sides
for
first
time.
Experimental
investigations
showed
that
only
emitted
ECL
signals
at
electrodes
with
positive
polarity,
regardless
whether
or
negative
voltage
was
applied.
Inspired
by
this,
we
introduced
carbon
vacancy-modified
iron
single-atom
catalyst
(VC-Fe-N-C
SAC)
excellent
oxygen
reduction
reaction
(ORR)
activity
into
working
electrode,
can
catalyze
dissolved
O2
produce
abundant
reactive
species
(ROS).
ROS
diffused
surface
counter
oxidize
high-intensity
signal
an
ultralow
trigger
potential.
As
proof-of-concept
application,
sensitive
biosensor
constructed
microcystin-LR
(MC-LR)
detection.
This
solved
substance
traditional
system
improved
accuracy
sensitivity.
Furthermore,
introduction
catalysts
(SAC)
avoided
use
coreactant
H2O2
tedious
electrochemical
oxidation
process
luminol,
broadened
application
biosensors.
Biosensors and Bioelectronics X,
Journal Year:
2024,
Volume and Issue:
19, P. 100514 - 100514
Published: July 5, 2024
Nanozyme
cascade
have
garnered
substantial
interest
in
recent
years
due
to
their
distinctive
properties.
However,
the
conventional
stepwise
reaction
undergoes
tedious
two-step
operation
process
owing
incompatibility
of
conditions.
Moreover,
most
reported
nanozymes
exhibit
favorable
catalytic
performance
only
acidic
medium,
which
greatly
restricts
usage
especially
biochemical
analysis.
To
address
above
challenges,
we
developed
gold
nanoparticles/calcium
hexacyanoferrate
(Ⅲ)/nitrogen-doped
graphitic
alkyne
(AuNPs/CaHF
NPs/N-GDY)
nanozyme
with
superior
activity
at
neutral
pH
comparable
that
acidic.
Specifically,
AuNPs/CaHF
NPs/N-GDY
simultaneously
possessed
glucose
oxidase-like
(GOx)
and
peroxidase-like
(HRP)
activities,
could
induce
one-step
presence
glucose,
resulting
5-fold
enhancement
efficiency
compared
reaction.
Besides,
tripedal
DNA
walker
was
equipped
sufficient
walking
legs
walk
on
directional
highly
controllable
stepped
track,
reducing
possibility
derailment
boosting
efficiency.
As
a
proof
concept,
novel
electrochemical
biosensor
constructed
for
miRNA-21
sensitive
detection
physiological
pH,
successfully
applied
human
serum
samples
as
well
practical
intracellular
analysis,
offering
great
potential
biomedical
research
clinical
diagnosis.
