Engineering an Ionic Aggregation-Induced Luminescence-Labeled Fluorescence Lateral Flow Immunoassay for C-Reactive Protein in Human Plasma DOI

Pei-Yuan Sun,

Q. Li, Ning Gao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 565 - 573

Published: Dec. 27, 2024

The surge of lateral flow immunoassays (LFAs) stimulates researchers to explore the novel vibrant aggregation-induced emission luminogen (AIEgen)-doped nanoparticles improve accuracy and reliability LFAs. However, loading amount AIEgens currently used for LFA in microspheres is limited due their symmetrical large conjugated skeleton structure, which significantly reduces fluorescence brightness signal reporter LFA. Herein, an ionic with a donor–acceptor type was developed as C-reactive protein (CRP). Ionic are unable enter hydrophobic cavity polystyrene (PS) because low nature. By altering extended alkyl chains, it possible increase hydrophobicity, thereby potentially increasing capacity within PS. Notably, fluorescent (denoted AIETPANPs) formed by embedding (E)-4-(4-(diphenylamino)styryl)-1-octadecylpyridin-1-ium iodide (TPA) PS showed orange-red have high quantum yield. Anti-CRP antibody (mAb1) could be effectively surface AIETPANPs amino-carboxyl reaction, resulting AIETPANPs-mAb1. AIETPANPs-mAb1 exhibited at 613 nm, point detectable naked eye minimal background interference. entire analysis accomplished just 10 min, achieving limit detection 4.06 ng/mL CRP. AIETPANPs-mAb1-based demonstrates excellent stability specificity fully meets requirements clinical diagnosis.

Language: Английский

Research progress on detection of foodborne pathogens: The more rapid and accurate answer to food safety DOI

Run-Jian Gao,

Xinxin Liu,

Zhiqiang Xiong

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 193, P. 114767 - 114767

Published: July 29, 2024

Language: Английский

Citations

21
Nucleic Acid Aptamer‐Based Sensors for Bacteria Detection: A Review DOI Open Access

Yalan Tang,

Yun Li, Ping Chen

et al.

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Bacteria have a significant impact on human production and life, endangering life health, so rapid detection of infectious agents is essential to improve health. Aptamers, which are pieces oligonucleotides (DNA or RNA) been applied biosensors for bacteria due their high affinity, selectivity, robust chemical stability, compatibility with various signal amplification transduction mechanisms. In this review, we summarize the different bacterial aptamers selected in recent years using SELEX technology discuss differences optical electrochemical aptamer sensors. addition technological developments innovations sensor introduced. Combining new materials methods, efficiency stability sensors also improved. This review summarizes progress current based practical application status provides an outlook future development.

Language: Английский

Citations

0
Smartphones as a platform for molecular analysis: concepts, methods, devices and future potential DOI Creative Commons
D. G. Baker,

Jasmine Bernal-Escalante,

Christine Traaseth

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Smartphones have the potential to transform chemical and biological analysis by functioning as portable, versatile, democratized platforms for molecular diagnostics. Recent advances in optical detection devices are reviewed.

Language: Английский

Citations

0
Functionalized magnetic nanoparticles enrichment and nanoelectrospray ionization coupled with a miniature mass spectrometer: A broad-spectrum rapid bacterial discrimination platform DOI
Meng Chen, Baoqiang Li, Zhongyao Zhang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113024 - 113024

Published: Feb. 1, 2025

Language: Английский

Citations

0
Boronic acid-functionalized Fe3O4 nanoparticles for activity-preserved enrichment of low-abundance bacteria from real samples DOI Creative Commons
Jingwen Chen, Shaobo Li,

Bin Deng

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(7), P. 5507 - 5522

Published: Jan. 1, 2025

Fe 3 O 4 @poly(PEGDA- co -MAAPBA) NPs prepared for selective capture and enrichment of Gram-positive bacteria.

Language: Английский

Citations

0
Bifunctional phage@isoluminol microgels coupled with peroxidase-like activity phagomagnetic nanoparticles for ultrasensitive chemiluminescence detection and in-situ inactivation of Shewanella in seafood matrices DOI
Xi Liu,

Zixin Ming,

Yanchun Shao

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162267 - 162267

Published: April 1, 2025

Language: Английский

Citations

0
Developing oxygen vacancy-rich CuMn2O4/carbon dots dual-function nanozymes via Chan-Lam coupling reaction for the colorimetric/fluorescent determination of D-penicillamine DOI
Min Feng, Xiaodan Zhang, Yuming Huang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 267, P. 116864 - 116864

Published: Oct. 19, 2024

Language: Английский

Citations

2
Engineering an Ionic Aggregation-Induced Luminescence-Labeled Fluorescence Lateral Flow Immunoassay for C-Reactive Protein in Human Plasma DOI

Pei-Yuan Sun,

Q. Li, Ning Gao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 565 - 573

Published: Dec. 27, 2024

The surge of lateral flow immunoassays (LFAs) stimulates researchers to explore the novel vibrant aggregation-induced emission luminogen (AIEgen)-doped nanoparticles improve accuracy and reliability LFAs. However, loading amount AIEgens currently used for LFA in microspheres is limited due their symmetrical large conjugated skeleton structure, which significantly reduces fluorescence brightness signal reporter LFA. Herein, an ionic with a donor–acceptor type was developed as C-reactive protein (CRP). Ionic are unable enter hydrophobic cavity polystyrene (PS) because low nature. By altering extended alkyl chains, it possible increase hydrophobicity, thereby potentially increasing capacity within PS. Notably, fluorescent (denoted AIETPANPs) formed by embedding (E)-4-(4-(diphenylamino)styryl)-1-octadecylpyridin-1-ium iodide (TPA) PS showed orange-red have high quantum yield. Anti-CRP antibody (mAb1) could be effectively surface AIETPANPs amino-carboxyl reaction, resulting AIETPANPs-mAb1. AIETPANPs-mAb1 exhibited at 613 nm, point detectable naked eye minimal background interference. entire analysis accomplished just 10 min, achieving limit detection 4.06 ng/mL CRP. AIETPANPs-mAb1-based demonstrates excellent stability specificity fully meets requirements clinical diagnosis.

Language: Английский

Citations

2