Protein-Induced Electrochemiluminescence Enhancement of Tetraphenylvinyl Derivatives for Ultrasensitive Bioanalysis DOI
Pan Zhou, Xiaochun Zhu, Xiaofeng Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19473 - 19482

Published: Nov. 29, 2024

Herein, the bovine serum albumin (BSA)-loaded tetrakis[4-(4′-cyanophenyl)phenyl]ethane nanoaggregates (NAs) (BSA@TBPE-(CN)4 NAs) as a novel electrochemiluminescence (ECL) emitter were first prepared, which exhibited superior ECL performance via newly defined protein-induced enhancement. Impressively, BSA not only restricted intramolecular motions by its hydrophobic cavity to improve optical radiation for enhancing efficiency but also promoted electrochemical excitation of BSA@TBPE-(CN)4 NAs in amino acid residues altered surface states and narrowed energy gap further boosting efficiency. Furthermore, displayed more dispersed state due electrostatic repulsion caused considerable negative charges, was conducive reacting fully with coreactants improving emission. Excitingly, threefold stronger intensity 4.8-fold higher compared those TBPE-(CN)4 NAs. Thus, an application, biosensor fabricated based on efficient mismatch-fueled three-dimensional (3D) DNA walker effective signal amplifier rapid ultrasensitive detection microRNA-21 limit (LOD) 19.1 aM, it successfully applied assess expression human cancer cells MCF-7 HeLa. This work developed avenue reasonably synthesize highly organic emitters motivating their potential application biosensing high-resolution bioimaging.

Language: Английский

Visualization of protrusion-localized STAT3 mRNA using a self-powered lipidic nanoflare for predicting hepatocellular carcinoma metastasis DOI Creative Commons
Ya Zhang, Ruichao Zeng, Yuanhang Xia

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 18, 2025

Cancer cell metastasis is one of the major causes patients death with hepatocellular carcinoma (HCC). Previous findings demonstrated that protrusion-accumulated STAT3 mRNA highly related to HCC metastasis, making protrusion-localized an ideal biomarker for evaluating initiation and progression. A self-powered lipidic nanoflare (SLNF) has been developed detecting expression level in individual cells, which enables accurate prediction metastasis. The LNF system a cholesterol micelle decorated two kinds DNA probes, double-stranded response single-stranded fuel probe. can be easily assembled from amphipathic cholesterol-conjugated via hydrophobicity-mediated aggregation, exhibiting efficient internalization. Moreover, compact high-density arrangement probes on surface enhances their biostability. All above features make approach intracellular RNA imaging. assay commences binding DNA, peels off waste exposes toehold domain. This domain serves as proximal holding point probe initiate strand displacement amplification, crucial step enabling detection targets expressed at trace levels, yielding limit (LOD) 100 pM 37 °C within 1.5 h. SLNF expected provide useful insight into development simple degradation-resistant visual showing potential applications tumor diagnosis treatment.

Language: Английский

Citations

0
Near-infrared light-responsive DNA nanosensor with hyperbranched self-stacking exponential amplification for sensitive and precise live-cell and in vivo imaging DOI
Weilin Chen, Zhuo Chen, Tiantian Zhang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 428, P. 137259 - 137259

Published: Jan. 16, 2025

Language: Английский

Citations

0
A Primer-Regulated Rolling Circle Amplification (RCA) for Logic-Controlled Multiplexed Enzyme Analysis DOI
Shuiqin Chai, Weimin Sun, Xin Hou

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

DNA-related enzymes are associated with various diseases and have been potential biomarkers for clinical diagnosis. Developing robust ultrasensitive methods is extremely favorable the detection of these biomarkers. To this purpose, a primer-regulated rolling circle amplification (RCA) strategy was ingeniously proposed. Briefly, RCA primer, which invalidated 3'-inverted dT (locked state) unable to initiate an reaction by phi29 DNA polymerase, embedded recognition substrate specific enzyme. In presence target, cleavage process enzyme prompted release regeneration 3'-OH (unlocked state), satisfying vital prerequisite RCA. By adopting programmable modular design, can be either single base sites or sequence different types enzymes. This also enables us conduct multiple conveniently, relying on logic-controlled manner including YES, OR, AND, AND-OR operations. Overall, proposed uniquely insightful provides universal tool analyses diverse

Language: Английский

Citations

0
Dual-palindrome-incorporated hand-in-hand self-linking bidirectional DNA amplifier within exogenous near-infrared light stimulation for high-performance imaging in living biosystems DOI
Tiantian Zhang, Xiaoming Sun, Weilin Chen

et al.

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 128003 - 128003

Published: March 26, 2025

Language: Английский

Citations

0
Controlled activation of a DNA walker driven by mismatched catalytic hairpin assembly for microRNA imaging in cancer cells and clinical samples DOI
Liuting Mo,

Shiya Tang,

Fenghua Meng

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 437, P. 137747 - 137747

Published: April 10, 2025

Language: Английский

Citations

0
Multienzymatic Orthogonal Activation of DNA Codec Enables Tumor-Specific Imaging of Base Excision Repair Activity DOI

Weijia Zeng,

Zhaopeng Chen, Yan‐Mei Lei

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(40), P. 15915 - 15923

Published: Sept. 26, 2024

Accurate monitoring of base excision repair (BER) activity in cancer cells is critical for advancing the comprehension DNA processes, gaining insights into development, and guiding treatment strategies. However, current assay techniques assessing BER face challenges due to heterogeneous origins diversity enzymes. In this work, we present a hi

Language: Английский

Citations

2
Combined Detection of Hepatitis B Virus Surface Antigen and Hepatitis B Virus DNA using DNA sensor DOI
Hang Gong,

Shufen Yao,

Yong Li

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(43), P. 7319 - 7324

Published: Jan. 1, 2024

A multi-detection strategy based on branched DNA nanostructures was developed to achieve the simultaneous high specificity identification and highly sensitive fluorescence responses HBV antigen DNA.

Language: Английский

Citations

1
Acidic Extracellular pH-Activated Allosteric DNA Nanodevice for Fluorescence Imaging of APE1 Activity in Tumor Cells DOI
Hui He, Yuchen Wu, Mingjian Chen

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18079 - 18085

Published: Oct. 30, 2024

Allostery is a phenomenon where the binding of ligand at one allosteric site influences affinity for another an active site. Different from orthosteric regulation, it allows more precise control biomolecular activity and enhances stability molecules. Inspired by regulation natural molecules, we present Y-shaped DNA nanodevice, termed YssAP, that was pH-responsive functionalized with AS1411 aptamer accurate fluorescence imaging human apurinic/apyrimidinic endonuclease (APE1) in tumor cells. With rational design, YssAP could not be cut APE1, Cy5 proximity BHQ2, leading to suppressed signal emission. In contrast, since acidic pH acted as effector, underwent conformational change into activated probe (YdsAP) extracellular pH. After entering cell via specific recognition aptamer, overexpressed APE1 AP on YdsAP. moved far away resulting strong output. Compared direct construction substrate, nanodevices have effects, which can precisely adjusted changing switching state. We anticipate this strategy will applied screening inhibitors diagnosis.

Language: Английский

Citations

1
Protein-Induced Electrochemiluminescence Enhancement of Tetraphenylvinyl Derivatives for Ultrasensitive Bioanalysis DOI
Pan Zhou, Xiaochun Zhu, Xiaofeng Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19473 - 19482

Published: Nov. 29, 2024

Herein, the bovine serum albumin (BSA)-loaded tetrakis[4-(4′-cyanophenyl)phenyl]ethane nanoaggregates (NAs) (BSA@TBPE-(CN)4 NAs) as a novel electrochemiluminescence (ECL) emitter were first prepared, which exhibited superior ECL performance via newly defined protein-induced enhancement. Impressively, BSA not only restricted intramolecular motions by its hydrophobic cavity to improve optical radiation for enhancing efficiency but also promoted electrochemical excitation of BSA@TBPE-(CN)4 NAs in amino acid residues altered surface states and narrowed energy gap further boosting efficiency. Furthermore, displayed more dispersed state due electrostatic repulsion caused considerable negative charges, was conducive reacting fully with coreactants improving emission. Excitingly, threefold stronger intensity 4.8-fold higher compared those TBPE-(CN)4 NAs. Thus, an application, biosensor fabricated based on efficient mismatch-fueled three-dimensional (3D) DNA walker effective signal amplifier rapid ultrasensitive detection microRNA-21 limit (LOD) 19.1 aM, it successfully applied assess expression human cancer cells MCF-7 HeLa. This work developed avenue reasonably synthesize highly organic emitters motivating their potential application biosensing high-resolution bioimaging.

Language: Английский

Citations

1