High Selectivity Fluorescence and Electrochemical Dual-Mode Detection of Glutathione in the Serum of Parkinson’s Disease Model Mice and Humans

Analytical Chemistry, Journal Year: 2025, Volume and Issue: 97(2), P. 1318 - 1328

Published: Jan. 9, 2025

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and accumulation alpha-synuclein. Glutathione (GSH), key antioxidant, significantly depleted in PD patients. This study presents dual-mode detection strategy for selectively determining GSH using single probe. A series "turn-on" electrochemical fluorescent probes were developed, with resorufin (Re) serving as reporting unit featuring specific recognition sites. Among these, 7-(3,5-dinitrophenoxy)-3H-phenoxazin-3-one (Re-DNP) probe was selected its high selectivity both Its response to superior comparison that observed hydrogen sulfide (H2S) cysteine (Cys). For screen-printed carbon electrode (SPCE)/carbon nanotube (CNT) modified electrodes, limit 5 μM, linear range 25-500 μM. In fluorescence detection, produced 78-fold increase emission at 630 nm presence GSH, strong correlation between intensity concentration 10-700 2 When applied real clinical serum samples, demonstrated lower levels mice human patients compared healthy controls. method provides sensitive accurate tool potential applications understanding GSH's role related diseases.

Language: Английский

---

Enhancing Clinical Precision in Lung Cancer Tissue Biopsy through Elevated Response-Threshold of an Endoplasmic Reticulum-Targeted Fluorogenic Probe

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101654 - 101654

Published: March 11, 2025

Lung carcinoma is the leading cause of mortality globally, posing a significant public health concern. Fluorescent-mediated tumor imaging emerging as novel diagnostic and therapeutic approach in clinical practice. Nevertheless, traditional probes lack accuracy diagnosing tumors due to overlap baseline values certain biomarkers between normal cells both exhibit turn-on fluorescence, rendering it impossible distinguish tissue from with high resolution. We introduce sensing strategy that constructs probe an elevated biomarker response-threshold targeting ability for endoplasmic reticulum (ER), enabling precise distinction cells, successfully develop such probe. Elevating advantageous minimizing interference cells. Additionally, ER ensures probe's response range consistent content ER, collectively enhancing differentiation cancer Using this probe, distinct bright fluorescence signal could be observed confocal tissues tumor-bearing mice after intravenous injection, stark contrast limited emanating tissues. Furthermore, demonstrated exceptional precision distinguishing lung para-cancer tissue. This work presents more reliable detection strategy, capable accurate diagnosis even when highly expressed It promises valuable tool future applications, particularly intraoperative assisted resection.

Language: Английский

---

Smartphone-Based Bimetallic Single-Atom Nanozyme Sensor Array Integrated with Deep Learning for Rapid Biothiol Detection

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Language: Английский

---

Organic Molecules as a Bridge Connecting Photoelectrochemistry and Fluorescence for Dual-Signal Assay

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

We report a strategy based on pyridyl-anchored organic small-molecule fluorescent probes to develop dual-signal sensing platform. The accomplishes an intelligent integration of fluorescence analysis with photoelectrochemical (PEC) sensing, thereby enabling rapid and precise detection hypochlorite. In this work, the natural dye chromone was selected as fluorophore for generating signals. Meanwhile, by using phenothiazine (PTZ) specific recognition group pyridine anchoring moiety, we designed synthesized novel probe. obtained probe used photosensitive material anchored TiO2 surface via N → Ti bonds, form FTO/TiO2/FPTZ-1 heterostructure-based platform This has characteristics high specificity, sensitivity, ease preparation, qualitative readout quantitative hypochlorite, limit 0.288 μM 1.37 nM PEC.

Language: Английский

---

Asymmetric and Symmetric S-zig-zag-Fused BODIPYs: Synthesis and Photophysical and Oxidative Properties

The Journal of Organic Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

We present a new, straightforward, and versatile approach that utilizes regioselective brominated precursors to synthesize both asymmetric symmetric S-zig-zag-fused BODIPYs (s-TFB bis-TFB) in moderate yields (45% 40%, respectively). X-ray structure analyses reveal the planar rigidity of BODIPY skeleton is progressively enhanced with an increasing number thiopyran rings. The annulation S-heteroaromatic rings at zig-zag edge core results blue-shifted absorption emission spectra, bis-TFB exhibiting maxima 530 539 nm elevated LUMO energy levels. In contrast, oxidation s-TFB m-CPBA demonstrates significant site selectivity, affording four products, namely s-s-SFB, s-bis-SFB, bis-s-SFB, bis-bis-SFB, ranging from 22% 36%. These oxidated derivatives display large red-shifted spectra (e.g., 648 735 for s-bis-SFB), along more stable HOMO levels reduced HOMO-LUMO gaps. This cyclization/oxidation strategy enables precise tuning optoelectronic properties, opening new avenues dye design application.

Language: Английский

---

FRET-Based Nanoprobe with Adaptive Background Suppression for Reliable Detection of ONOO^–/ClO^– in Whole Blood: Facilitating Monitoring of Sepsis Progression and Hemolytic Disorders

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abnormal fluctuations in blood biomarker levels serve as critical indicators of the disease. However, detecting endogenous substances whole using fluorescent probes is challenging due to its complex composition. This challenge primarily arises from two factors: high autofluorescence and intrinsic fluorescence probe, both contributing significant background detection system. To overcome these obstacles, we introduced a donor–acceptor "one-to-many" FRET-based sensing strategy integrated with suppression design multifunctional nanoprobe. The donor effectively suppresses through inner filter effect efficiently quenches by adjusting acceptor-to-donor ratio, achieving "zero" detection. Leveraging this excellent quenching effect, successfully detected ONOO– ClO– samples mice sepsis or hemolytic diseases. Furthermore, monitored changes throughout disease course, revealing positive correlation between concentrations severity. innovative for provides valuable insights designing directly detect blood.

Language: Английский

---

Illuminating Cisplatin-induced Ferroptosis in Non-Small-Cell Lung Cancer with Biothiols-Activable Fluorescent/ Photoacoustic Bimodal Probes

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Using activatable NIR fluorescent/PA bimodal probes, we successfully monitored real-time biothiol changes during ferroptosis in NSCLC cells and tumors, revealing enhanced with the combined use of erastin cisplatin.

Language: Английский

---