Nano Letters,
Journal Year:
2024,
Volume and Issue:
24(9), P. 2912 - 2920
Published: Feb. 23, 2024
Nanozymes
with
peroxidase-like
activity
have
been
extensively
studied
for
colorimetric
biosensing.
However,
their
catalytic
and
specificity
still
lag
far
behind
those
of
natural
enzymes,
which
significantly
affects
the
accuracy
sensitivity
To
address
this
issue,
we
design
PdSn
nanozymes
selectively
enhanced
activity,
improves
a
immunoassay.
The
is
higher
than
that
Pd
nanozymes.
Theoretical
calculations
reveal
p–d
orbital
hybridization
Sn
not
only
results
in
an
upward
shift
d-band
center
to
enhance
hydrogen
peroxide
(H2O2)
adsorption
but
also
regulates
O–O
bonding
strength
H2O2
achieve
selective
activation.
Ultimately,
nanozyme-linked
immunosorbent
assay
has
successfully
developed
sensitively
accurately
detect
prostate-specific
antigen
(PSA),
achieving
low
detection
limit
1.696
pg
mL–1.
This
work
demonstrates
promising
approach
detecting
PSA
clinical
diagnosis.
Chemical Reviews,
Journal Year:
2019,
Volume and Issue:
119(6), P. 4357 - 4412
Published: Feb. 25, 2019
Because
of
the
high
catalytic
activities
and
substrate
specificity,
natural
enzymes
have
been
widely
used
in
industrial,
medical,
biological
fields,
etc.
Although
promising,
they
often
suffer
from
intrinsic
shortcomings
such
as
cost,
low
operational
stability,
difficulties
recycling.
To
overcome
these
shortcomings,
researchers
devoted
to
exploration
artificial
enzyme
mimics
for
a
long
time.
Since
discovery
ferromagnetic
nanoparticles
with
horseradish
peroxidase-like
activity
2007,
large
amount
studies
on
nanozymes
constantly
emerging
next
decade.
Nanozymes
are
one
kind
nanomaterials
enzymatic
properties.
Compared
enzymes,
advantages
stability
durability,
which
fields.
A
thorough
understanding
possible
mechanisms
will
contribute
development
novel
high-efficient
nanozymes,
rational
regulations
great
significance.
In
this
review,
we
systematically
introduce
classification,
mechanism,
regulation
well
recent
research
progress
field
biosensing,
environmental
protection,
disease
treatments,
past
years.
We
also
propose
current
challenges
their
future
focus.
anticipate
review
may
be
significance
understand
properties
mimicking
activities.
Angewandte Chemie International Edition,
Journal Year:
2019,
Volume and Issue:
59(7), P. 2565 - 2576
Published: June 18, 2019
Nanomaterials
with
enzyme-like
activities,
coined
nanozymes,
have
been
researched
widely
as
they
offer
unparalleled
advantages
in
terms
of
low
cost,
superior
activity,
and
high
stability.
The
complex
structure
composition
nanozymes
has
led
to
extensive
investigation
their
catalytic
sites
at
an
atomic
scale,
in-depth
understanding
the
biocatalysis
occurring.
Single-atom
catalysts
(SACs),
characterized
by
atomically
dispersed
active
sites,
provided
opportunities
for
mimicking
metalloprotease
bridging
gap
between
natural
enzymes
nanozymes.
In
this
Minireview,
we
illustrate
unique
properties
discuss
recent
advances
synthesis,
characterization,
applications
SACs.
Subsequently,
outline
impressive
progress
made
single-atom
sensing,
degradation
organic
pollutants,
therapeutic
roles.
Finally,
present
major
challenges
remaining
a
successful
marriage
Journal of Medicinal Chemistry,
Journal Year:
2019,
Volume and Issue:
63(1), P. 1 - 22
Published: Sept. 10, 2019
Inherently
conducting
polymers
(ICPs)
are
a
specific
category
of
synthetic
with
distinctive
electro-optic
properties,
which
involve
conjugated
chains
alternating
single
and
double
bonds.
Polyaniline
(PANI),
as
one
the
most
well-known
ICPs,
has
outstanding
potential
applications
in
biomedicine
because
its
high
electrical
conductivity
biocompatibility
caused
by
hydrophilic
nature,
low-toxicity,
good
environmental
stability,
nanostructured
morphology.
Some
limitations
use
PANI,
such
low
processability
degradability,
can
be
overcome
preparation
blends
nanocomposites
various
(bio)polymers
nanomaterials,
respectively.
This
review
describes
state-of-the-art
biological
activities
conductive
PANI-based
biomedical
fields,
antimicrobial
therapy,
drug
delivery,
biosensors,
nerve
regeneration,
tissue
engineering.
The
latest
progresses
reviewed
to
provide
background
for
future
research.
Analytical Chemistry,
Journal Year:
2019,
Volume and Issue:
91(3), P. 2447 - 2454
Published: Jan. 4, 2019
This
work
presented
an
innovative
and
rationally
engineered
palindromic
molecular
beacon
(PMB)
based
"Z-scheme"
photoelectrochemical
(PEC)
biosensing
protocol
for
the
selective
screening
of
kanamycin
(Kana)
through
DNA
hybridization-induced
conformational
conversion.
Interestingly,
ingeniously
designed
PMB
integrated
multifunctional
elements
including
recognition
region,
primer-like
fragment,
polymerization-nicking
template.
The
cosensitized
structures
consisted
CdS
quantum
dot
functionalized
hairpin
DNA2
(QD-HP2)
region-selectively
deposited
gold
nanoparticles
onto
{001}
facets
bismuth
oxychloride
(BiOCl-Au).
Compared
with
BiOCl-Au
alone,
attachment
QDs
(i.e.,
BiOCl-Au-CdS
QDs)
exhibited
evidently
enhanced
photocurrent
intensity
thanks
to
synergistic
effect
Z-scheme
QDs.
After
incubation
target
Kana,
Kana-aptamer
binding
could
induce
exposure
region
DNA1
(HP1).
exposed
tails
hybridized
each
other
(like
a
machine)
consume
substrates
(dNTPs)
fuels
(enzyme)
releasing
numerous
nick
fragments
(Nick).
as-generated
specifically
hybridize
complementary
QD-HP2,
thus
resulting
in
decreasing
because
increasing
spatial
distance
electron
transfer
between
two-type
photosensitizers.
Under
optimum
conditions,
PMB-based
sensing
system
satisfying
responses
toward
Kana
within
working
range
from
50
5000
fM
at
low
detection
limit
29
fM.
Impressively,
concept
fragment-mediated
primer-free
strategy
offers
new
avenue
advanced
development
efficient
convenient
biodetection
systems.
Microsystems & Nanoengineering,
Journal Year:
2023,
Volume and Issue:
9(1)
Published: Jan. 1, 2023
Abstract
Flexible
wearable
sweat
sensors
allow
continuous,
real-time,
noninvasive
detection
of
analytes,
provide
insight
into
human
physiology
at
the
molecular
level,
and
have
received
significant
attention
for
their
promising
applications
in
personalized
health
monitoring.
Electrochemical
are
best
choice
due
to
high
performance,
low
cost,
miniaturization,
wide
applicability.
Recent
developments
soft
microfluidics,
multiplexed
biosensing,
energy
harvesting
devices,
materials
advanced
compatibility
electrochemical
sweat-sensing
platforms.
In
this
review,
we
summarize
potential
medical
methods
stimulation
collection.
This
paper
provides
an
overview
components
recent
power
supply
technologies
highlights
some
typical
sensing
platforms
different
types
analytes.
Finally,
ends
with
a
discussion
challenges
view
prospective
development
exciting
field.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(20), P. 7442 - 7448
Published: May 12, 2022
This
work
presented
a
point-of-care
(POC)
photoelectrochemical
(PEC)
biosensing
for
the
detection
of
human
papillomavirus-16
(HPV-16)
on
portable
electrochemical
system
by
using
CRISPR-Cas12a
trans-cleaving
G-quadruplex
biorecognition/amplification
and
hollow
In2O3-In2S3-modified
screen-printed
electrode
(In2O3-In2S3/SPE)
as
photoactive
material.
G-quadruplexes
were
capable
biocatalytic
precipitation
(H2O2-mediated
4-chloro-1-naphthol
oxidation)
In2O3-In2S3/SPE
surface,
resulting
in
weakened
photocurrent,
but
suffered
from
trans-cleavage
when
specifically
recognized
analyte.
The
photocurrent
results
could
be
directly
observed
with
card-sized
device
via
smartphone,
which
displayed
high-value
these
positive
samples,
while
low-value
target-free
samples.
Such
exhibited
satisfying
responses
toward
HPV-16
within
wide
working
range
5.0
to
5000
pM
allowed
at
concentration
low
1.2
pM.
proposed
assay
provided
smartphone
signal
readout
enable
rapid
screening
PEC
determination
without
sophisticated
instruments,
thus
meeting
requirements
remote
areas
resource-limited
settings.
We
envision
that
combining
an
efficient
biometric
sensing
platform
wireless
will
high-throughput
POC
diagnostic
analysis.
Small,
Journal Year:
2019,
Volume and Issue:
15(42)
Published: Sept. 16, 2019
Nucleic
acids
are
considered
as
perfect
programmable
materials
for
cascade
signal
amplification
and
not
merely
genetic
information
carriers.
Among
them,
catalytic
hairpin
assembly
(CHA),
an
enzyme-free,
high-efficiency,
isothermal
method,
is
a
typical
example.
A
CHA
reaction
initiated
by
single-stranded
analytes,
substrate
hairpins
successively
opened,
resulting
in
thermodynamically
stable
duplexes.
circuits,
which
were
first
proposed
2008,
present
dozens
of
systems
today.
Through
in-depth
research
on
mechanisms,
the
circuits
have
been
continuously
enriched
with
diverse
improved
analytical
performance.
After
short
time,
can
realize
exponential
under
conditions.
Under
certain
conditions,
even
achieve
600
000-fold
amplification.
Owing
to
its
promising
versatility,
able
be
applied
analysis
various
markers
vitro
living
cells.
Also,
integrated
nanomaterials
other
molecular
biotechnologies
produce
readouts.
Herein,
varied
designs,
conditions
introduced
detail.
Additionally,
biosensors
based
presented.
Finally,
challenges
outlook
development
considered.
