Designing Fluorescent Interfaces at Hotspots in a Plasmonic Nanopore for Homologous Optoelectronic Sensing DOI Open Access

Li‐Dong Chen,

Di Zhang,

Zi‐Hui Li

et al.

Small, Journal Year: 2025, Volume and Issue: 21(9)

Published: Jan. 20, 2025

In this work, a site-selective functionalization strategy is proposed for modifying fluorescent dyes in the plasmonic nanopore, which highlights building optoelectronic dual-signal sensing interfaces at "hotspots" locations to construct multiparameter detection nanosensor. Finite-difference time-domain (FDTD) simulations confirmed high-intensity electromagnetic field due nanostructure. It demonstrated that adjusting distance between nanopore inner wall and fluorophore prevented fluorescence quenching, resulting more than thirty fold enhancement. Upon binding with target analyte, sensor produces homologous yet independent responses cross-validate one another, providing highly accurate analysis even presence of multiple interferences. The platform demonstrates precise, adaptable linear extracellular pH changes single-cell level, making it versatile tool range biosensing applications. By enabling metal nanopores, interface design efficiently exploits enhancement fields achieve high-precision measurements greatly improves sensitivity

Language: Английский

Machine Learning-Based Label-Free SERS Profiling of Exosomes for Accurate Fuzzy Diagnosis of Cancer and Dynamic Monitoring of Drug Therapeutic Processes DOI

Xingkang Diao,

Xinli Li,

Shuping Hou

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(19), P. 7552 - 7559

Published: May 4, 2023

Exosomes are a class of extracellular vesicles secreted by cells, which can be used as promising noninvasive biomarkers for the early diagnosis and treatment diseases, especially cancer. However, due to heterogeneity exosomes, it remains grand challenge distinguish accurately reliably exosomes from clinical samples. Herein, we achieve accurate fuzzy discrimination human serum samples breast cancer cervical through machine learning-based label-free surface-enhanced Raman spectroscopy (SERS), using "hot spot" rich 3D plasmonic AuNPs nanomembranes substrates. Due existence some weak distinguishable SERS fingerprint signals high sensitivity method, analysis precisely identify three (normal cancerous) cell lines, two different types without specific labeling biomarkers. The prediction accuracy based on learning algorithm was up 91.1% lines (H8, HeLa, MCF-7 cell)-derived exosomes. Our model trained with spectra cell-derived could reach 93.3% Furthermore, action mechanism chemotherapeutic process cells revealed dynamic monitoring profiling secreted. method would useful postoperative assessment or other diseases in future.

Language: Английский

Citations

69
Pro-Oxidant Therapeutic Activities of Cerium Oxide Nanoparticles in Colorectal Carcinoma Cells DOI Creative Commons
Aparna Datta,

Snehasis Mishra,

Krishnendu Manna

et al.

ACS Omega, Journal Year: 2020, Volume and Issue: 5(17), P. 9714 - 9723

Published: April 26, 2020

Given that basal levels of reactive oxygen species (ROS) are higher in cancer cells, there is a growing school thought endorses pro-oxidants as potential chemotherapeutic agents. Intriguingly, cerium oxide (CeO2) nanoparticles can manifest either anti- or pro-oxidant activity function differential pH various subcellular localizations. In an acidic environment, for example, extracellular milieu CeO2 would pro-oxidant. Based on this concept, the present study designed to investigate activities human colorectal carcinoma cell line (HCT 116). For comparison, we have also studied effect ceria embryonic kidney (HEK 293) cells. Dose-dependent viability cancerous well normal cells has been assessed by treating them independently with different concentrations (5–100 μg/mL) culture media. The half maximal inhibitory concentration (IC50) nanoceria HCT 116 found be 50.48 μg/mL while HEK 293 92.03 μg/mL. To understand intricate molecular mechanisms CeO2-induced cellular apoptosis, series experiments conducted. apoptosis-inducing ability investigated Annexin V-FITC staining, caspase 3/9 analysis, cytochrome c release, intracellular ROS and mitochondrial membrane analysis using flow cytometry. Experimental data suggest treatment causes DNA fragmentation through enhanced generation ROS, which ultimately leads apoptosis p53-dependent signaling pathway.

Language: Английский

Citations

99
Visualizing Mitochondrial Form and Function within the Cell DOI
Brian Glancy

Trends in Molecular Medicine, Journal Year: 2019, Volume and Issue: 26(1), P. 58 - 70

Published: Nov. 6, 2019

Language: Английский

Citations

77
Construction of a magnetic-fluorescent-plasmonic nanosensor for the determination of MMP-2 activity based on SERS-fluorescence dual-mode signals DOI
Lin Liu, Hongyu Chu,

Jukun Yang

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 212, P. 114389 - 114389

Published: May 20, 2022

Language: Английский

Citations

47
The nanocapsules with dual-targeting of cell and mitochondrial function for enhanced hypoxia-activated drug therapy DOI
Fen Li, Ziyi Li, Jing Zhao

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

CZPA NC, which targets cell and mitochondrial function, can increase intracellular hypoxia activate a drug to produce radicals, causing DNA damage, dysfunction, changes in morphology.

Language: Английский

Citations

1
Recent advances in nanoscale metal-organic frameworks biosensors for detection of biomarkers DOI
Qiuyang Xu, Zheng Tan, Xuewei Liao

et al.

Chinese Chemical Letters, Journal Year: 2021, Volume and Issue: 33(1), P. 22 - 32

Published: June 11, 2021

Language: Английский

Citations

51
Metal-organic frameworks for pharmaceutical and biomedical applications DOI

Tooba Rezaee,

Reihaneh Fazel-Zarandi,

Afsaneh Karimi

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2022, Volume and Issue: 221, P. 115026 - 115026

Published: Sept. 5, 2022

Language: Английский

Citations

34
Optical and Electrochemical Probes for Monitoring Cytochrome c in Subcellular Compartments During Apoptosis DOI Creative Commons
Zhijuan Duan, Yu Ouyang,

Yuzhe Fu

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(18)

Published: March 6, 2023

Abstract Cytochrome c (Cyt. ) is a key initiator of the caspases that activate cell apoptosis. The spatiotemporal evaluation contents Cyt. in cellular compartments and detection delivery between upon apoptosis important for probing viabilities. We introduce an optical probe electrochemical quantitative assessment at single level. or probes are functionalized with photoresponsive o‐nitrobenzylphosphate ester‐caged aptamer constituents. These uncaged by light stimuli compartments, allowing through formation /aptamer complexes non‐apoptotic apoptotic conditions. applied to distinguish epithelial MCF‐10A breast cells malignant MCF‐7 MDA‐MB‐231 under apoptotic/non‐apoptotic

Language: Английский

Citations

21
Plasmonic SERS Au Nanosunflowers for Sensitive and Label-Free Diagnosis of DNA Base Damage in Stimulus-Induced Cell Apoptosis DOI
Guohua Qi, Dandan Wang, Chuanping Li

et al.

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 92(17), P. 11755 - 11762

Published: Aug. 11, 2020

Molecular diagnosis and accurate damage analysis of complex genomic DNAs in tumor cells are crucial to the theranostics cancers but still a huge challenge. Herein, by designed preparation uniform plasmonic sunflower-like assembly gold (Au) nanostructure that is capable efficient DNA capture providing high-density gap-plasmon "hot spots" for adequate surface-enhanced Raman spectroscopy (SERS) enhancement, we succeeded sensitive reliable label-free SERS detection electrostimulus-induced apoptotic cancer at base level first time. The results showed external electrostimulus (at 1.2 V, 5 min) was almost harmless normal healthy cells, it caused pronounced double strand break adenine cell DNAs, which effectively destroyed reproduction transcription ultimately induced apoptosis. developed sensing platform method promising study genetically related diseases.

Language: Английский

Citations

48
Single-cell ATP detection and content analyses in electrostimulus-induced apoptosis using functionalized glass nanopipettes DOI
Dandan Wang, Guohua Qi, Ya Zhou

et al.

Chemical Communications, Journal Year: 2020, Volume and Issue: 56(10), P. 1561 - 1564

Published: Jan. 1, 2020

Functionalized glass nanopipettes were applied for single-cell ATP detection through partial disassembly of gold nanoparticle assemblies after combination with ATP.

Language: Английский

Citations

44