Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by using single gold nanoelectrodes DOI
Hao Wang,

Binbin Yang,

Haoran Tang

et al.

The Analyst, Journal Year: 2023, Volume and Issue: 148(22), P. 5636 - 5641

Published: Jan. 1, 2023

A new sensor has been developed to detect miRNA-15 using nanoelectrodes and a hairpin DNA-based electrochemical amplification technique. By utilizing complex DNA cylinder connected with DNA1, the is able absorb more methylene blue (MB) than simple double-stranded DNA. Another DNA2 modified on an Au nanoelectrode surface and, when introduced, it triggers chain reaction. This reaction unlocks two hairpins alternatively polymerize into structure that attaches MB. The then replaced by DNA1 due strand displacement reactions continues react next achieve circular amplification. signal from MB oxidation linear relationship concentrations, making possible miRNA-15. Moreover, this method can be readily adapted for detection of various other miRNA species. newly devised nanosensor holds promising applications in vivo within biological systems, which achieved leveraging advantageous characteristics nanoelectrodes, including their low resistance-capacitance time constant, rapid mass transfer kinetics, small diameter.

Language: Английский

Light-activated CRISPR-Cas12a for amplified imaging of microRNA in cell cycle phases at single-cell levels DOI Creative Commons

Hong Liu,

Jiantong Dong,

Zhijuan Duan

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(30)

Published: July 24, 2024

An ortho-nitrobenzyl phosphate ester-caged nucleic acid hairpin structure coupled to the CRISPR-Cas12a complex is introduced as a functional reaction module for light-induced activation of (LAC12a) machinery toward amplified fluorescence detection microRNA-21 (miRNA-21). The LAC12a applied selective, in vitro sensing miRNA-21 and intracellular imaging different cell lines. system used image cycle phases MCF-7 cells. Moreover, integrated cells enables two-photon laser confocal microscopy-assisted, light-stimulated spatiotemporal, selective at single-cell level evaluation relative expression levels distinct phases. method implemented map distribution an array single

Language: Английский

Citations

13
Aggregation-Enabled Electrochemistry in Confined Nanopore Capable of Complementary Faradaic and Non-Faradaic Detection DOI

Si-Hao Wu,

Shuang-Chen Zhang,

Yu-Han Kang

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(14), P. 4241 - 4247

Published: March 28, 2024

Electrochemistry that empowers innovative nanoscopic analysis has long been pursued. Here, the concept of aggregation-enabled electrochemistry (AEE) in a confined nanopore is proposed and devised by reactive oxygen species (ROS)-responsive aggregation CdS quantum dots (QDs) within functional nanopipette. Complementary Faradaic non-Faradaic operations QDs aggregate could be conducted to simultaneously induce signal-on photocurrents signal-off ionic signals. Such rationale permits cross-checking mutually corroborated signals thus delivers more reliable results for single-cell ROS analysis. Combined with rich biomatter–light interplay, AEE can extended other stimuli-responsive aggregations electrochemical innovations.

Language: Английский

Citations

11
Cathepsin B Activatable Fluorescent Probe for Antitumor Efficiency Feedback: Attempt To Detect Certain Apoptotic Cells DOI
Qiqi Yu, Dongdong Xu, Sina Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

As many treatments kill tumor cells by inducing apoptosis, fluorescent probes that can detect apoptosis are crucial for effective feedback regarding therapy outcomes (in particular, activatable better imaging). Cathepsins enzymes released from lysosomes into the cytoplasm during lysosomal membrane permeabilization-induced of cells, making them potential biomarkers apoptotic cells. Despite their potential, to best our knowledge, no cathepsin-activatable have been reported this purpose. To fill gap, we designed a cathepsin B (CTSB)-activatable probe, A-DCO, which distinguish normal in free state and give enhanced fluorescence signal under drug stimulation when encapsulated nanoparticles (NPs), enabling evaluation treatment efficacy. proof concept, A-DCO NPs were employed rather than Annexin-V/PI double staining 4T1 MCF-7 via flow cytometry. In vivo experiments demonstrated rapidly quickly metabolized after imaging, achieving timely, accurate, safe about effectiveness. The study offers new tool detecting certain provides inspiration direction probe design future.

Language: Английский

Citations

1
Light-activated aptasensor for plug-and-play detection of Aflatoxin B1 in food samples DOI

Yongmei Jia,

Bing Han, Guohua Zhou

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1346, P. 343786 - 343786

Published: Feb. 9, 2025

Language: Английский

Citations

1
Nature-inspired design of droplet-synthesized polymeric nanoelectrode for photoelectrochemical microRNA sensing within single cells DOI
Siyuan Yu, Yili Liu, Zheng Li

et al.

Science Bulletin, Journal Year: 2023, Volume and Issue: 69(2), P. 159 - 162

Published: Nov. 10, 2023

Language: Английский

Citations

19
Self‐Assembly Hypoxic and ROS Dual Response Nano Prodrug as a New Therapeutic Approach for Glaucoma Treatments DOI Creative Commons
Xuezhi Zhou, Rong Rong,

Ganghao Liang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(41)

Published: Sept. 4, 2024

Abstract Glaucoma is an irreversible blinding eye disease characterized by retinal ganglion cell (RGC) death.Previous studies have demonstrated that protecting mitochondria and activating the CaMKII/CREB signaling pathway can effectively protect RGC axon. However, currently treatments are often unsatisfactory, pathogenesis of glaucoma requires further elucidation. In this study, a ROS‐responsive dual drug conjugate (OLN monomer) first designed simultaneously bonds nicotinamide oleic acid. The self‐assembled into nanoparticles (uhOLN‐NPs) through aggregation multiple micelles possesses ROS scavenging capability. Then, polymer with hypoxic response function designed, which encapsulates uhOLN‐NPs to form responses (HOLN‐NPs). Under hypoxia in RGCs, azo bond HOLN‐NPs breaks releases uhOLN‐NPs. Meanwhile, under high conditions, thioketone broke, leading dissociation nano‐prodrug. released acid co‐scavenge activate pathway, RGCs. exhibit significantly superior protective effect on R28 cells glutamate models glaucoma. accumulation RGCs lead significant inhibition apoptosis axonal damage vivo. Notably, provide new therapeutic approach for patients neurodegenerative disease.

Language: Английский

Citations

5
θ-Nanopore Ratiometry DOI
Haiyan Wang, Bing Wang, Chao Sun

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(5), P. 4551 - 4558

Published: Jan. 24, 2024

Developing nanoscale ratiometric techniques capable of biochemical response should prove significance for precise applications with stringent spatial and biological restrictions. Here we present devise the concept θ-nanopore ratiometry, which uses signals that could well address serious concerns about device deviation in fabrication nonspecific adsorption detection. As exemplified by a 200 nm toward miRNA detection, ±20 aperture drift be mitigated issue minimized complex cytosolic environment. Practical application this ratiometry realizes measurements miRNA-10b. This work has not only established nanoscopic technique but also enriched extant armory nanotools single-cell studies beyond.

Language: Английский

Citations

4
Bridging Ionic Current Rectification and Resistive-Pulse Sensing for Reliable Wide-Linearity Detection DOI
Xian Zhang, Zeng‐Qiang Wu,

You‐Wei Zheng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(16), P. 6444 - 6449

Published: April 10, 2024

As two mainstream ionic detection techniques, current rectification (ICR) suffers from large fluctuations in trace level detection, while resistive-pulse sensing (RPS) encounters easy clogs high-concentration detection. By rationally matching the nanopore size with DNA tetrahedron (TDN), this work bridges techniques to achieve reliable wide linearity. a representative analyte, miRNA-10b could specifically combine and release TDN interior wall, which thus induced simultaneous generation of distinct ICR RPS signals. The signals be attributed balance between effective orifice surface charge density inner were by complex passing through nanopore. Such an operation contributed range 1 fM-1 nM good feasibility method is also validated single-cell real plasma

Language: Английский

Citations

4
A review of innovative electrochemical strategies for bioactive molecule detection and cell imaging: Current advances and challenges DOI
Hao Zhang, Hui Jiang, Xiaohui Liu

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1285, P. 341920 - 341920

Published: Oct. 19, 2023

Language: Английский

Citations

10
Promoting the transition from pyroptosis to apoptosis in endothelial cells: a novel approach to alleviate methylglyoxal-induced vascular damage DOI Creative Commons

Ruqiang Yuan,

Hu Xu, Mingqi Wang

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 10, 2025

Methylglyoxal (MGO)-induced cell death in vascular endothelial cells (VECs) plays a critical role the progression of diabetic complications (DVCs). Previous studies have shown that MGO can induce inflammatory pyroptosis, leading to VEC damage. However, underlying mechanism remains unclear, and effective interventions are yet be developed. Human umbilical vein (HUVECs) were used for vitro experiments. Cell modes assessed through morphological observations. Mechanistic investigations performed using immunofluorescence, flow cytometry, Western blotting, ELISA. Inhibitors adenoviruses employed validate mechanisms. Vascular organoids conjunction with AngioTool plug-in assays evaluate damage angiogenic capacity. Mouse blood pressure was measured tail-cuff method, morphology examined hematoxylin eosin (H&E) staining as well immunofluorescence staining. Data analyzed GraphPad Prism software. Our study revealed induces pyroptosis VECs via Caspase3/gasdermin E (GSDME) pathway. Furthermore, saponin monomer 13 dwarf lilyturf tuber (DT-13), inhibited MGO-induced promoted generation apoptotic bodies, facilitating transition from apoptosis. Mechanistically, DT-13 suppressed Caspase3-mediated cleavage GSDME non-muscle myosin heavy chain IIA (NMMHC IIA), while increasing phosphorylation light 2 (MLC2), which facilitated body formation. Additionally, mitigate damage, inhibit angiogenesis, reduce remodeling, alleviate hypertension. This uncovers novel highlighting therapeutic significance apoptosis this process. These findings suggest potential strategies managing angiopathy. emerges promising compound intervention, offering new possibilities clinical applications.

Language: Английский

Citations

0