RSC Chemical Biology, Journal Year: 2024, Volume and Issue: 5(4), P. 273 - 292
Published: Jan. 1, 2024
This comprehensive review article discusses the potential of cell – silent Raman region, reporting on tags employed for biomedical applications using variants spectroscopy.
Language: Английский
Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 54(18), P. 11322 - 11332
Published: Aug. 19, 2020
The increasing and simultaneous pollution of plastic debris antibiotic resistance in aquatic environments makes plastisphere a great health concern. However, the development process resistome is largely unknown, impeding risk assessment associated with plastics. Here, we profiled temporal dynamics genes (ARGs), mobile genetic elements (MGEs), microbial composition from initial colonization to biofilm formation urban water. A total 82 ARGs, 12 MGEs, 63 bacterial pathogens were detected categorized as pioneering, intermediate, persistent ones. high number five MGEs six ARGs persistently whole was regarded major concern because their potential role disseminating resistance. In addition genomic analysis, D2O-labeled single-cell Raman spectroscopy employed interrogate ecophysiology culture-independent way demonstrated that inherently more tolerant antibiotics than bacterioplankton. Finally, by combining intensified pathogenic bacteria, increased tolerance antibiotic, trophic transfer into holistic indicated constitute hot spot acquire spread impose long-term ecosystems human health. These findings provide important insights ecological highlight necessity for comprehensive surveillance plastisphere.
Language: Английский
Citations
183
Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(13), P. 9221 - 9230
Published: June 17, 2021
UV irradiation and chlorination have been widely used for water disinfection. However, there are some limitations, such as the risk of generating viable but nonculturable bacteria reactivation when using or alone. This study comprehensively evaluated feasibility UV/chlorine process in drinking disinfection, Pseudomonas aeruginosa was selected target microorganism. The number culturable cells effectively reduced by more than 5 orders magnitude (5-log10) after UV, chlorine, treatments. intact VBNC were detected at 103 to 104 cells/mL chlorine treatments, whereas they undetectable treatment due primary contribution reactive species (Cl•, Cl2•-, ClO•). After treatment, metabolic activity determined single cell Raman spectroscopy much lower that UV. level toxic opr gene P. decreased 99% treatment. Importantly, bacterial dark completely suppressed not chlorination. suggests can damage is promising pathogen inactivation overcome limitations treatments
Language: Английский
Citations
159
Sensors and Actuators B Chemical, Journal Year: 2020, Volume and Issue: 320, P. 128350 - 128350
Published: May 26, 2020
Language: Английский
Citations
131
Current Opinion in Biotechnology, Journal Year: 2020, Volume and Issue: 64, P. 218 - 229
Published: July 17, 2020
Language: Английский
Citations
92
Advanced Science, Journal Year: 2020, Volume and Issue: 7(19)
Published: Aug. 16, 2020
Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with appropriate antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging deuterium oxide (D2 O) metabolism reported. Metabolic incorporation D2 O into biomass in single bacterium metabolic response to are probed as short 10 min after culture 70% medium, fastest among current technologies. Single-cell inactivation concentration (SC-MIC) obtained less than 2.5 h from colony results. The SC-MIC 37 sets bacterial isolate samples, which include 8 major species 14 different often encountered clinic, validated standard minimal inhibitory blindly measured via broth microdilution. Toward clinical translation, determination 1 antibiotic treatment 30 mixture incubation bacteria urine or whole blood demonstrated.
Language: Английский
Citations
91
Critical Reviews in Food Science and Nutrition, Journal Year: 2021, Volume and Issue: 62(16), P. 4294 - 4308
Published: July 12, 2021
The conventional microbial cell analyses are mostly population-averaged methods that conceal the characteristics of single-cell in community. Single-cell analysis can provide information on functional and structural variation each cell, resulting elimination long tedious cultivation techniques. Raman spectroscopy is a label-free, noninvasive, in-vivo method ideal for measurement to obtain spatially resolved chemical information. In current review, recent developments spectroscopic techniques characterization at level presented, focusing imaging single cells study intracellular distribution different components. review also discusses limitation challenges technique put forward some future outlook improving spectroscopy-based analysis. have potential precision measurements, metabolic analysis, antibiotic susceptibility testing, resuscitation capability, correlating phenotypic genomics cells, integration with other such as microfluidics, stable isotope probing (SIP), atomic force microscope further improve resolution extensive Future focuses should be given advance algorithms data standardized reference libraries, automated isolation future.
Language: Английский
Citations
81
TrAC Trends in Analytical Chemistry, Journal Year: 2020, Volume and Issue: 127, P. 115891 - 115891
Published: April 7, 2020
Language: Английский
Citations
76
Nature Food, Journal Year: 2021, Volume and Issue: 2(4), P. 233 - 240
Published: April 21, 2021
Language: Английский
Citations
75
Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(12), P. 5098 - 5106
Published: March 17, 2021
Human health is at great risk due to the spreading of antimicrobial resistance (AMR). The lengthy procedure conventional susceptibility testing (AST) usually requires a few days. We developed fast Raman-assisted antibiotic test (FRAST), which detects single bacterial metabolic activity in presence antibiotics, using Raman single-cell spectroscopy. It was found that spectra (SCRS) would show clear and distinguishable band "silent zone" (2000–2300 cm–1), active incorporation deuterium from heavy water (D2O) by antibiotic-resistant bacteria. This pilot study has compared FRAST AST for six clinical standard quality controls (four Gram-negative two Gram-positive bacteria strains) response 38 antibiotics. In total, 3200 treatments have been carried out approximately 64 000 SCRS acquired analysis. result showed an overall agreement 88.0% between assay. gram-staining classification based on linear discriminant analysis (LDA) model developed, seamlessly coupling with further reduce turnaround time. applied real nine urinary infectious samples three sepsis samples. results were consistent MALDI-TOF identification AST. Under optimal conditions, "sample report" could be reduced 3 h urine 21 provides reliable tests, speed up microbiological practice facilitate stewardship.
Language: Английский
Citations
65
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2022, Volume and Issue: 276, P. 121217 - 121217
Published: April 1, 2022
Metabolic dynamics of bacterial cells is needed for understanding the correlation between changes in environmental conditions and cell metabolic activity. In this study, Raman spectroscopy combined with deuterium labelling was used to analyze activity a single Escherichia coli O157:H7 cell. The incorporation from heavy water into cellular biomolecules resulted formation carbon-deuterium (CD) peaks spectra, indicating broad vibrational corresponding CD CH encompassed different specific shifts macromolecules such as protein, lipids, nucleic acid. utilization tryptophan oleic acid by sole carbon source led lipid composition, indicated new second derivative spectra. Thus, proposed method could semi-quantitatively determine total activity, macromolecule identification, protein metabolism
Language: Английский
Citations
52