Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(27), P. 9724 - 9731
Published: June 28, 2022
As
a
golden
partner
of
recombinase
polymerase
amplification
(RPA),
CRISPR/Cas12a
has
been
proven
to
solve
the
false-positive
problem
caused
by
nonspecific
perfectly;
meanwhile,
its
trans-cleave
activity
further
enhanced
sensitivity.
However,
solution
transfer
operation
after
tube
cap
opening
greatly
increases
risk
aerosol
contamination
amplicon,
which
is
inconsistent
with
point-of-care
(POC)
diagnostics
requirements.
This
study
proposes
photoactivated
strategy
achieve
one-pot
high-sensitivity
nucleic
acid
detection.
Using
photocleavable
complementary
ssDNA
block
crRNA,
RPA
can
smoothly
pass
through
exponential
interval
without
being
affected
activated
Cas12a
in
critical
early
stage.
After
enough
amplicons
were
produced,
test
was
short
bursts
ultraviolet
radiation
at
365
nm.
method
achieved
sensitivity
2.5
copies
within
40
min.
simple
and
sensitive
effectively
avoid
amplicon
lower
threshold
for
molecular
POC.
Microsystems & Nanoengineering,
Journal Year:
2023,
Volume and Issue:
9(1)
Published: Jan. 1, 2023
Abstract
Flexible
wearable
sweat
sensors
allow
continuous,
real-time,
noninvasive
detection
of
analytes,
provide
insight
into
human
physiology
at
the
molecular
level,
and
have
received
significant
attention
for
their
promising
applications
in
personalized
health
monitoring.
Electrochemical
are
best
choice
due
to
high
performance,
low
cost,
miniaturization,
wide
applicability.
Recent
developments
soft
microfluidics,
multiplexed
biosensing,
energy
harvesting
devices,
materials
advanced
compatibility
electrochemical
sweat-sensing
platforms.
In
this
review,
we
summarize
potential
medical
methods
stimulation
collection.
This
paper
provides
an
overview
components
recent
power
supply
technologies
highlights
some
typical
sensing
platforms
different
types
analytes.
Finally,
ends
with
a
discussion
challenges
view
prospective
development
exciting
field.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(20), P. 7442 - 7448
Published: May 12, 2022
This
work
presented
a
point-of-care
(POC)
photoelectrochemical
(PEC)
biosensing
for
the
detection
of
human
papillomavirus-16
(HPV-16)
on
portable
electrochemical
system
by
using
CRISPR-Cas12a
trans-cleaving
G-quadruplex
biorecognition/amplification
and
hollow
In2O3-In2S3-modified
screen-printed
electrode
(In2O3-In2S3/SPE)
as
photoactive
material.
G-quadruplexes
were
capable
biocatalytic
precipitation
(H2O2-mediated
4-chloro-1-naphthol
oxidation)
In2O3-In2S3/SPE
surface,
resulting
in
weakened
photocurrent,
but
suffered
from
trans-cleavage
when
specifically
recognized
analyte.
The
photocurrent
results
could
be
directly
observed
with
card-sized
device
via
smartphone,
which
displayed
high-value
these
positive
samples,
while
low-value
target-free
samples.
Such
exhibited
satisfying
responses
toward
HPV-16
within
wide
working
range
5.0
to
5000
pM
allowed
at
concentration
low
1.2
pM.
proposed
assay
provided
smartphone
signal
readout
enable
rapid
screening
PEC
determination
without
sophisticated
instruments,
thus
meeting
requirements
remote
areas
resource-limited
settings.
We
envision
that
combining
an
efficient
biometric
sensing
platform
wireless
will
high-throughput
POC
diagnostic
analysis.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(11), P. 4859 - 4865
Published: March 9, 2022
Exploiting
innovative
sensing
mechanisms
and
their
rational
implementation
for
selective
sensitive
detection
has
recently
become
one
of
the
mainstream
research
directions
photoelectrochemical
(PEC)
bioanalysis.
In
contrast
to
existing
conventional
strategies,
this
study
presents
a
new
liposome-mediated
method
via
in
situ
combining
ZnInS
nanosheets
(ZIS
NSs)
with
SnS2
form
ZIS
NSs/SnS2
type-I
heterojunction
on
fluorine-doped
tin
oxide
(FTO)
electrodes
highly
PEC
immunoassays.
Specifically,
alkaline
phosphatase
(ALP)-encapsulated
liposomes
were
confined
within
96-well
plates
by
sandwich
immunorecognition
subsequently
subjected
lysis
treatment.
Enzymatically
produced
H2S
released
ALP
was
then
directed
react
Sn(IV)
engender
FTO/ZIS
NSs-Sn(IV)
electrode,
resulting
change
photogenerated
electron–hole
transfer
path
photoelectrode
reduction
current
signaling.
Exemplified
heart-type
fatty
acid
binding
protein
(h-FABP)
as
target,
constructed
sensor
showed
good
stability
selectivity
biosensing
system.
Under
optimal
conditions,
as-prepared
platform
displayed
high
sensitivity
h-FABP
dynamic
linear
response
range
0.1–1000
pg/mL
lower
limit
55
fg/mL.
This
immunoassay
based
establishment,
providing
protocol
analyzing
various
targets
interest.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(39), P. 13389 - 13397
Published: Sept. 23, 2021
Early
diagnosis
of
cancers
relies
on
the
sensitive
detection
specific
biomarkers,
but
most
current
testing
methods
are
inaccessible
to
home
healthcare
due
cumbersome
steps,
prolonged
time,
and
utilization
toxic
hazardous
substances.
Herein,
we
developed
a
portable
self-powered
photoelectrochemical
(PEC)
sensing
platform
for
rapid
prostate-specific
antigen
(PSA,
as
model
disease-related
protein)
by
integrating
photoelectric
signal
output
system
catalyzed
with
chemiluminescence-functionalized
Au
nanoparticles
(AuNPs)
phosphomolybdic
acid
(PMA)-based
photochromic
visualization
platform.
TiO2-g-C3N4-PMA
photosensitive
materials
were
first
synthesized
functionalized
sensor
chip.
The
consisted
filter
paper
modified
photocatalytic
material
regional
laser-etched
FTO
electrode
an
alternative
conventional
PEC
glass-based
electrode.
targeting
involved
monoclonal
anti-PSA
capture
antibody-functionalized
Fe3O4
magnetic
bead
(mAb1-MB)
polyclonal
antibody
(pAb2)-N-(4-aminobutyl)-N-ethylisoluminol-AuNP
(ABEI-AuNP).
Based
intensity
chemiluminescent
system,
device
color
changed
from
light
yellow
heteropoly
blue
through
PMA
integrated
into
output.
In
addition,
electrical
in
was
amplified
sandwich-type
capacitor
readout
handheld
digital
multimeter.
Under
optimum
conditions,
exhibited
high
sensitivity
relative
PSA
range
0.01–50
ng
mL–1
low
limit
6.25
pg
mL–1.
flow-through
chemiluminescence
reactor
semiautomatic
injection
separation
avoid
unstable
source
inherent
process.
Therefore,
our
strategy
provides
new
horizon
point-of-care
analysis
cost-effective
clinical
diagnosis.
Electroanalysis,
Journal Year:
2021,
Volume and Issue:
33(8), P. 1827 - 1851
Published: May 19, 2021
Abstract
Electroanalysis
has
obtained
considerable
progress
over
the
past
few
years,
especially
in
field
of
electrochemical
sensors.
Broadly
speaking,
sensors
include
not
only
conventional
biosensors
or
non‐biosensors,
but
also
emerging
electrochemiluminescence
(ECL)
and
photoelectrochemical
(PEC)
which
are
both
combined
with
optical
methods.
In
addition,
various
sensing
devices
have
been
developed
for
practical
purposes,
such
as
multiplexed
simultaneous
detection
disease‐related
biomarkers
non‐invasive
body
fluid
monitoring.
For
further
performance
improvement
sensors,
material
is
crucial.
Recent
a
kind
two‐dimensional
(2D)
nanomaterial
MXene
containing
transition
metal
carbides,
nitrides
carbonitrides,
unique
structural,
mechanical,
electronic,
optical,
thermal
properties,
attracted
lot
attention
form
analytical
chemists,
widely
applied
Here,
we
reviewed
based
on
from
Nov.
2014
(when
first
work
about
sensor
published)
to
Mar.
2021,
dividing
them
into
different
types
biosensors,
flexible
We
believe
this
review
will
be
help
those
who
want
design
develop
MXene,
hoping
new
inspirations
could
sparked.
