Tuning Star Polymer Architecture to Tailor Secondary Structures and Mechanical Properties of Diblock Polypeptide Hydrogels for Direct Ink Writing DOI Creative Commons

Muireann Cosgrave,

Kulwinder Kaur, Christopher D. Simpson

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 670 - 678

Published: Dec. 19, 2024

Hydrogel three-dimensional (3D) printing has emerged as a highly valuable fabrication tool for applications ranging from electronics and biomedicine. While conventional hydrogels such gelatin, alginate, hyaluronic acid satisfy biocompatibility requirements, they distinctly lack reproducibility in terms of mechanical properties 3D printability. Aiming to offer high-performance alternative, here we present range amphiphilic star-shaped diblock copolypeptides l-glutamate l-leucine residues with different topologies. Hydrophobic side chains the polymer block drive conformational self-assembly water, spontaneously forming tunable properties, through variation star topology. Their amenable shear-thinning self-recovery render them suitable hydrogel inks direct ink writing. Well-defined 3D-printed structures can be readily generated rapidly photo-cross-linked using visible light (405 nm) after methacrylamide functionalization, while demonstrate good top-seeded encapsulated MC3T3 cells.

Language: Английский

Whole-body aging mediates the association between exposure to volatile organic compounds and osteoarthritis among U.S. middle-to-old-aged adults DOI
Hao‐Long Zhou, Dongsheng Di, Zhangbo Cui

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 907, P. 167728 - 167728

Published: Oct. 11, 2023

Language: Английский

Citations

13
Exploring Local Reactivity of Large Systems through Combining Conceptual DFT and the GFN2-xTB Method DOI
Ricardo Pino‐Rios

The Journal of Physical Chemistry A, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

This study evaluates the ability of GFN2-xTB method and Conceptual Density Functional Theory-derived tools to predict local reactivity in large systems. Carbon-based systems such as C60, C70, Li+@C70, C240, C360, C648, C720 have been used test sets, orbital-weighted dual descriptor was employed identify nucleophilic electrophilic regions, providing a comprehensive analysis their patterns. The results confirm that accurately reproduces profiles observed experimentally at DFT level, particularly well-known fullerenes like C60 C70. addition an endohedral Li+ cation C70 demonstrated enhanced electrophilicity reduced unfavorable consistent with previous studies. For larger less-studied systems, C720, revealed distinct features, including localization regions −C≡C– units C240/C648, ends C360 nanoparticle model, emergence zones due reduction aromaticity benzenoid rings C720. These findings validate computationally efficient alternative for exploring structures contribute valuable insights into potential applications molecular design material science nanotechnology.

Language: Английский

Citations

0
Semi-synthetic flavonoid derivatives from Boesenbergia Rotunda induce extrinsic apoptosis pathway via Caspase-3 and Caspase-8 in HCT116 Colon Cancer cell lines DOI
Atchara Janthong, Kraikrit Utama, Nopawit Khamto

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 159, P. 108343 - 108343

Published: March 9, 2025

Language: Английский

Citations

0
Lycium ruthenicum anthocyanins alleviate acrylamide-induced neurotoxicity in SH-SY5Y cells by mitigating ferroptosis via the Nrf2/GPX4 signaling pathway DOI
Qingqing Luo, Xuemei Qin,

H Y Guo

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106415 - 106415

Published: March 1, 2025

Language: Английский

Citations

0
The effect of polyol co-solvents on the gelation properties of polyvinyl alcohol-based hydrogels for cell immobilization DOI Creative Commons
Grant Keet, Tobias M. Louw, Robert W. M. Pott

et al.

Soft Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: March 24, 2025

Hydrogel materials have a variety of applications, ranging from biomedical and drug delivery systems, to their employment as immobilization matrices in bioprocessing applications. Polyvinyl alcohol (PVA) is synthetic, water-soluble, biocompatible polymer. Due its physical properties when gelled, this polymer has been highlighted suitable candidate for application the industry an matrix microorganisms. This study investigates effects using various polyols co-solvent on gelation PVA-based hydrogels formed at ambient conditions, resulting properties. In addition glycerol, previously known capabilities improve used co-solvent, including erythritol (C4H10O4), xylitol (C5H12O5), sorbitol (C6H14O6) were investigated. Key investigated, including: transparency; tensile compressive strengths; diffusion coefficients; rates gelation; method gelation. It was determined that are through crosslinking rather than chemical crosslinking. The glycerol-PVA tended exhibit more photosynthetic organisms, although differences between glycerol-PVA, xylitol-PVA, sorbitol-PVA comparable often not significantly different after rehydration. investigation showed simple polyols, solid could be conditions without requirement cytotoxic chemicals, harsh or unfavorable intermediate chemicals.

Language: Английский

Citations

0
Polysaccharides from Brasenia schreberi alleviated the toxicity induced by acrylamide on cells and Caenorhabditis elegans DOI
Yujie Wang,

Bangguo Wang,

Kuan Yan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142831 - 142831

Published: April 1, 2025

Language: Английский

Citations

0
Associations of exposure to volatile organic compounds with biological aging: a cross-sectional study DOI Creative Commons
X M Shi,

YiNi Wang,

Fei Yang

et al.

BMC Public Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 22, 2025

Language: Английский

Citations

0
A responsive living material prepared by diffusion reveals extracellular enzyme activity of cyanobacteria DOI Creative Commons
Lisa Tang, Nathan T. Soulier, Rebecca Wheeler

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(18)

Published: May 1, 2025

Stimuli-responsive engineered living materials (ELMs) can respond to environmental or biochemical cues and have broad utility in biological sensors machines, but traditionally been limited biocompatible scaffolds. This is because they are typically made by mixing cells into a precursor solution before crosslinking. Here, we demonstrate diffusion mechanism for incorporating of the cyanobacterium Synechococcus elongatus sp. PCC 7942 (S. elongatus) nanoclay-poly-N-isopropylacrylamide (NC-PNIPAm), hydrogel with cytotoxic precursor, exploiting its temperature-dependent shape-morphing behavior. Subsequent growth S. caused decrease bending curvature stiffness (local Young's modulus) NC-PNIPAm due partial degradation an unannotated enzyme. Creation observation this cyanobacteria-hydrogel ELM showcases method diffusing as well characterizing extracellular

Language: Английский

Citations

0
Species Difference? Bovine, Trout, and Human Plasma Protein Binding of Per- and Polyfluoroalkyl Substances DOI Creative Commons
Weiping Qin, Beate I. Escher, Julia Huchthausen

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(23), P. 9954 - 9966

Published: May 28, 2024

Per- and polyfluoroalkyl substances (PFAS) strongly bind to proteins lipids in blood, which govern their accumulation distribution organisms. Understanding the plasma binding mechanism species differences will facilitate quantitative

Language: Английский

Citations

1
3D Bioprintable Self-Healing Hyaluronic Acid Hydrogel with Cysteamine Grafting for Tissue Engineering DOI Creative Commons

Kasula Nagaraja,

Amitava Bhattacharyya,

Minsik Jung

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(12), P. 780 - 780

Published: Nov. 28, 2024

The abundance of hyaluronic acid (HA) in human tissues attracts its thorough research tissue regenerating scaffolds and 3D bioprintable hydrogel preparation. Though methacrylation HA can lead to photo-crosslinkable hydrogels, the catalyst has toxicity concerns, is not suitable for creating stable complex structures using extrusion bioprinting. In this study, a dual crosslinking on methacrylated introduced, cysteamine-grafted varying concentrations 2-hydroxy ethyl acrylate. resultant printing (or bioprinting), mechanically robust, self-standing, phosphate-buffered saline at 37 °C more than 42 days, high water absorption capacity with low swelling ratio (1.5), exhibits self-healing adhesive properties. Complex like ears pyramid shapes 2 cm height are printed optimized composition. All synthesized hydrogels have shown nontoxicity cell-supportiveness. Loading cells, tetracycline, bovine serum albumin into led better bioink properties such as cell attachment, growth, proliferation osteoblast cells. test results suggest that biocompatible potential bioprinting self-standing form engineering regenerative medicine.

Language: Английский

Citations

1