Electrostatic Regulation of Bola-Amphiphilic Peptide Self-Assembly and Nanotube Formation Induced by Salt Ions

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Peptide self-assembly into nanostructures offers substantial potential for applications in catalysis, drug delivery, and nanodevice fabrication. However, controlling the morphology of these assemblies remains a challenge. In this study, we investigate role salt ions regulating bola-amphiphilic peptide KFFFFK, facilitating transition from twisted fibrils to nanotubes. Using transmission electron microscopy, show that addition NaCl induces formation nanotubes, detail time-dependent assembly process. Increased concentrations reduce electrostatic repulsion, promoting lateral merging supporting closed This phenomenon is also observed with several other salts. These findings underscore critical interactions highlight importance concentration directing pathways. Our results provide valuable insights mechanisms offer versatile approach designing advanced biomaterials nanodevices based on well-defined nanostructures.

Language: Английский

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Intrinsically Disordered Peptide Nanofibers from A Structured Motif within Proteins

Angewandte Chemie International Edition, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Intrinsically disordered regions (IDRs) are ubiquitous in proteins, orchestrating complex cellular signaling through higher-order protein assemblies. However, the properties and functions of intrinsically peptide (IDP) assemblies largely underexplored. This work unveiled a facile strategy for engineering IDP We demonstrate that conjugating structured motif derived from protein's phosphorylation site to self-assembling tripeptide unexpectedly yields self-assembled nanofibers with intrinsic disorder. Specifically, by using glycine linker attach pentapeptide within random coil region SRC kinase C-terminus widely used enabler, we generated phosphorylated octapeptide. The octapeptide exhibits cell compatibility forms hydrogel upon dephosphorylation phosphooctapeptide. Cryo-electron microscopy (cryo-EM) structural analysis reveals peptides adopt two types helical arrangements but exhibit disorder at periphery nanofibers. hydrogels decreased adsorption increasing concentration. study represents first instance transitioning into an state nanofibers, expanding pool sequences IDPs providing valuable insights development cell-compatible biomaterials.

Language: Английский

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Atomic Insights Into Self‐Assembly of Zingibroside R1 and its Therapeutic Action Against Fungal Diseases

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract Natural products are a crucial resource for drug discovery, but poor understanding of the molecular‐scale mechanisms their self‐assembly into soluble, bioavailable hydrogels limits applications and therapeutic potential. It is demonstrated that Zingibroside R1 (ZR1), derived from Panax notoginseng, undergoes spontaneous self‐assemble hydrogel comprising helical nanofibrils with potent antifungal activity lacking in its monomeric state. Cryogenic electron microscopy (cryo‐EM) revealed an intricate hydrogen‐bonding network facilitates ZR1 nanofibril formation, characterized by hydrophobic core hydrophilic exterior architecture, which underpin binding cell wall vulvovaginal candidiasis (VVC) pathogen, C. albicans . The interface between gel glucan compromises membrane integrity, inhibiting proliferation vitro VVC model mice vivo. could also deliver probiotic Lactobacillus , synergistically restoring vaginal microenvironment. This study advances mechanistic ZR1's structure‐function relationships, offering valuable insights rational design optimization natural product‐based hydrogels.

Language: Английский

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Peptide Assemblies for Cancer Therapy

ChemMedChem, Journal Year: 2023, Volume and Issue: 18(17)

Published: June 29, 2023

Supramolecular assemblies made by the self-assembly of peptides are finding an increasing number applications in various fields. While early exploration peptide centered on tissue engineering or regenerative medicine, recent development has shown that can act as supramolecular medicine for cancer therapy. This review covers progress applying therapy, with emphasis works appeared over last five years. We start introduction a few seminal assemblies, then discuss combination anticancer drugs. Next, we highlight use enzyme-controlled transformation shapeshifting inhibiting cells and tumors. After that, provide outlook this exciting field promises new kind therapeutics

Language: Английский

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Enzymatic control of intermolecular interactions for generating synthetic nanoarchitectures in cellular environment

Science and Technology of Advanced Materials, Journal Year: 2024, Volume and Issue: 25(1)

Published: June 28, 2024

Nanoarchitectonics, as a technology to arrange nano-sized structural units such molecules in desired configuration, requires nano-organization, which usually relies on intermolecular interactions. This review briefly introduces the development of using enzymatic reactions control interactions for generating artificial nanoarchitectures cellular environment. We begin discussion with early examples and uniqueness enzymatically controlled self-assembly. Then, we describe intracellular nanostructures their relevant applications. Subsequently, discuss cases forming cell surface via reactions. Following that, highlight use creating intercellular nanostructures. Finally, provide summary outlook promises future direction this strategy. Our aim is give an updated introduction reaction regulating interactions, phenomenon ubiquitous biology but relatively less explored by chemists materials scientists. goal stimulate new developments simple versatile approach addressing societal needs.

Language: Английский

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