Reverse Transcription Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(8), P. 4126 - 4133

Published: Feb. 11, 2021

The outbreak of the pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) calls for an urgent unmet need developing a facial and cost-effective detection method. requirement well-trained personnel sophisticated instrument current primary mean (reverse transcription polymerase chain reaction, RT-PCR) may hinder practical application worldwide. In this regard, reverse recombinase amplification (RT-RPA) coupled with CRISPR-Cas12a colorimetric assay is proposed SARS-CoV-2 detection. methodology we have described herein utilizes DNA-modified gold nanoparticles (AuNPs) as universal readout can specifically target ORF1ab N regions genome. After virus genome amplified through RT-RPA, resulting abundant dsDNA will bind activate Cas12a. Under trans-cleavage degradation, capped DNA substrate be hydrolyzed gradually from AuNPs, demonstrating change in surface plasmon resonance (SPR), which facially monitored UV-vis absorbance spectroscopy naked eye observation. high efficiency RT-RPA Cas12a process bring sensitivity our method to 1 copy viral sequence per test. Notably, under dual variations inspecting isothermal activation process, false positive events other beta coronavirus members effectively avoided thus significantly improve specificity. Furthermore, reliability validated standard clinical samples hospital laboratory department. Through integration inherently specificity RPA-coupled system intrinsic simplicity AuNP-based assay, increases testing availability SARS-CoV-2.

Language: Английский

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Vapochromic crystals: understanding vapochromism from the perspective of crystal engineering

Chemical Society Reviews, Journal Year: 2020, Volume and Issue: 49(5), P. 1517 - 1544

Published: Jan. 1, 2020

Vapochromic crystals, a specific kind of vapochromic materials, can be investigated from the perspective crystal engineering to understand mechanism vapochromism, which is beneficial design and prepare task-specific materials.

Language: Английский

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Portable Food‐Freshness Prediction Platform Based on Colorimetric Barcode Combinatorics and Deep Convolutional Neural Networks

Advanced Materials, Journal Year: 2020, Volume and Issue: 32(45)

Published: Oct. 1, 2020

Abstract Artificial scent screening systems (known as electronic noses, E‐noses) have been researched extensively. A portable, automatic, and accurate, real‐time E‐nose requires both robust cross‐reactive sensing fingerprint pattern recognition. Few E‐noses commercialized because they suffer from either or pattern‐recognition issues. Here, colorimetric barcode combinatorics deep convolutional neural networks (DCNNs) are combined to form a system for monitoring meat freshness that concurrently provides The barcodes—comprising 20 different types of porous nanocomposites chitosan, dye, cellulose acetate—form fingerprints identifiable by DCNN. fully supervised DCNN trained using 3475 labeled images predicts with an overall accuracy 98.5%. Incorporating into smartphone application forms simple platform rapid scanning identification food in real time. is fast, non‐destructive, enabling consumers all stakeholders the supply chain monitor freshness.

Language: Английский

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Remote near infrared identification of pathogens with multiplexed nanosensors

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Nov. 25, 2020

Abstract Infectious diseases are worldwide a major cause of morbidity and mortality. Fast specific detection pathogens such as bacteria is needed to combat these diseases. Optimal methods would be non-invasive without extensive sample-taking/processing. Here, we developed set near infrared (NIR) fluorescent nanosensors used them for remote fingerprinting clinically important bacteria. The based on single-walled carbon nanotubes (SWCNTs) that fluoresce in the NIR optical tissue transparency window, which offers ultra-low background high penetration. They chemically tailored detect released metabolites well virulence factors (lipopolysaccharides, siderophores, DNases, proteases) integrated into functional hydrogel arrays with 9 different sensors. These hydrogels exposed clinical isolates 6 ( Staphylococcus aureus , Escherichia coli,…) (≥25 cm) imaging allows identify distinguish Sensors also spectrally encoded (900 nm, 1000 1250 nm) differentiate two P. aeruginosa S. penetrate (>5 mm). This type multiplexing enables differentiation potential smart surfaces.

Language: Английский

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Real-time monitoring of plant stresses via chemiresistive profiling of leaf volatiles by a wearable sensor

Matter, Journal Year: 2021, Volume and Issue: 4(7), P. 2553 - 2570

Published: July 1, 2021

Language: Английский

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Carbon dots based on natural resources: Synthesis and applications in sensors

Microchemical Journal, Journal Year: 2020, Volume and Issue: 160, P. 105604 - 105604

Published: Oct. 8, 2020

Language: Английский

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Microfluidic paper-based chips in rapid detection: Current status, challenges, and perspectives

TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 143, P. 116371 - 116371

Published: June 19, 2021

Language: Английский

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Wireless Self-Powered High-Performance Integrated Nanostructured-Gas-Sensor Network for Future Smart Homes

ACS Nano, Journal Year: 2021, Volume and Issue: 15(4), P. 7659 - 7667

Published: April 19, 2021

The accelerated evolution of communication platforms including Internet Things (IoT) and the fifth generation (5G) wireless network makes it possible to build intelligent gas sensor networks for real-time monitoring chemical safety personal health. However, this application scenario requires a challenging combination characteristics sensors small formfactor, low cost, ultralow power consumption, superior sensitivity, high intelligence. Herein, self-powered integrated nanostructured-gas-sensor (SINGOR) systems wirelessly connected SINGOR are demonstrated here. room-temperature operated system can be self-driven by indoor light with Si solar cell, features ultrahigh sensitivity H2, formaldehyde, toluene, acetone record limits detection (LOD) 10, 2, 1, 1 ppb, respectively. Each consisting an array nanostructured has capability pattern recognition classification. Furthermore, multiple as network, which successfully flammable leakage alarm function. They also achieve localization satisfactory precision when deployed in one single room. These successes promote development using wide range applications smart home/building future city.

Language: Английский

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Recent Progress in Amine Gas Sensors for Food Quality Monitoring: Novel Architectures for Sensing Materials and Systems

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(8), P. 2104 - 2131

Published: Aug. 1, 2022

The increasing demand for food production has necessitated the development of sensitive and reliable methods analysis, which allow optimization storage distribution while ensuring safety. Methods to quantify monitor volatile biogenic amines are key minimizing waste high-protein foods enable safe consumption fresh products. Novel materials device designs have allowed portable sensors that make use different transduction amine detection quality monitoring. Herein, we review past decade's advances in First, role as a food-quality index is presented. Moreover, comprehensive overview distinct gas provided according method, operation strategies, (e.g., metal oxide semiconductors, conjugated polymers, carbon nanotubes, graphene its derivatives, transition dichalcogenides, organic frameworks, MXenes, quantum dots, dyes, among others) employed each case. These include chemoresistive, fluorometric, colorimetric, microgravimetric sensors. Emphasis also given sensor arrays record fingerprints wireless devices operate radiofrequency identification (RFID) tags. Finally, challenges future opportunities on new presented aiming encourage further research technological reliable, integrated, remotely accessible

Language: Английский

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Surface-functionalized gold and silver nanoparticles for colorimetric and fluorescent sensing of metal ions and biomolecules

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 459, P. 214461 - 214461

Published: Feb. 15, 2022

Language: Английский

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