Activatable fluorescent probes for in situ imaging of enzymes

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 51(2), P. 450 - 463

Published: Dec. 24, 2021

As the main biomarkers of most diseases, enzymes play fundamental but extremely critical roles in biosystems. High-resolution studies using activatable situ fluorescence imaging may help to better elucidate their dynamics living systems. Currently, probes can realize changeable inevitably tend diffuse away from original active site enzyme and even translocate out cells, seriously impairing high-resolution observation enzymes. In be realized by labelling or antibodies with always-on signals that fail enable Thus, fluorescent both "activatable" "in situ" properties will this tutorial review, we summarize existing methods ranging design strategies bioimaging applications could used develop for It is expected review promote new generated such deciphering complex biosystems further extend application these other fields

Language: Английский

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Advanced bioactive nanomaterials for biomedical applications

Exploration, Journal Year: 2021, Volume and Issue: 1(3)

Published: Dec. 1, 2021

Bioactive materials are a kind of with unique bioactivities, which can change the cellular behaviors and elicit biological responses from living tissues. came into spotlight in late 1960s when researchers found that such as bioglass could react surrounding bone tissue for regeneration. In following decades, advances nanotechnology brought new development opportunities to bioactive nanomaterials. nanomaterials not simple miniaturization macroscopic materials. They exhibit bioactivities due their nanoscale size effect, high specific surface area, precise nanostructure, significantly influence interactions systems. Nowadays, have represented an important exciting area research. Current future applications ensure academic clinical importance. This review summaries recent field nanomaterials, evaluate factors bioactivities. Then, range potential biomedical discussed. Furthermore, limitations, challenges, also

Language: Английский

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Fluorescent-based nanosensors for selective detection of a wide range of biological macromolecules: A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 206, P. 115 - 147

Published: Feb. 26, 2022

Thanks to their unique attributes, such as good sensitivity, selectivity, high surface-to-volume ratio, and versatile optical electronic properties, fluorescent-based bioprobes have been used create highly sensitive nanobiosensors detect various biological chemical agents. These sensors are superior other analytical instrumentation techniques like gas chromatography, high-performance liquid capillary electrophoresis for being biodegradable, eco-friendly, more economical, operational, cost-effective. Moreover, several reports also highlighted application in the early detection of biomarkers associated with drug-induced organ damage liver, kidney, or lungs. In present work, we comprehensively overviewed electrochemical that employ nanomaterials (nanoparticles/colloids quantum dots, carbon nanoscaled metal-organic frameworks, etc.) a variety macromolecules based on fluorescent emission spectra. addition, most important mechanisms methods sense amino acids, protein, peptides, enzymes, carbohydrates, neurotransmitters, nucleic vitamins, ions, metals, electrolytes, blood gases, drugs (i.e., anti-inflammatory agents antibiotics), toxins, alkaloids, antioxidants, cancer biomarkers, urinary metabolites urea, uric acid, creatinine), pathogenic microorganisms were outlined compared terms selectivity sensitivity. Altogether, small dimensions capability these nanosensors sensitive, label-free, real-time sensing chemical, biological, pharmaceutical could be array-based screening in-vitro in-vivo diagnostics. Although nanoprobes widely applied determining macromolecules, unfortunately, they many challenges limitations. Efforts must made minimize limitations utilizing an emphasis commercial developments. We believe current review can foster wider incorporation nanomedicine will particular interest researchers working fluorescence technology, material chemistry, coordination polymers, related research areas.

Language: Английский

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Chemical Design of Activatable Photoacoustic Probes for Precise Biomedical Applications

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(6), P. 6850 - 6918

Published: March 2, 2022

Photoacoustic (PA) imaging technology, a three-dimensional hybrid modality that integrates the advantage of optical and acoustic imaging, has great application prospects in molecular due to its high depth resolution. To endow PA with ability for real-time visualization precise biomedical diagnosis, numerous activatable probes which can specifically alter their intensities upon reacting targets or biological events interest have been developed. This review highlights recent developments applications including detection biotargets events. First, generation mechanism signals will be given, followed by brief introduction contrast agents used probe design. Then we particularly summarize general design principles alteration strategies developing probes. Furthermore, give detailed discussion living systems. At last, current challenges outlooks future discussed. We hope this stimulate new ideas explore potentials future.

Language: Английский

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Monitoring the Fluctuation of Hydrogen Peroxide in Diabetes and Its Complications with a Novel Near-Infrared Fluorescent Probe

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(6), P. 3301 - 3307

Published: Feb. 4, 2021

Diabetes is one of the metabolic diseases marked by hyperglycemia and often accompanied occurrence some complications. As a biomarker oxidative stress, hydrogen peroxide (H2O2) has close association with development diabetes its Unfortunately, there no fluorescent probe reported for imaging H2O2 in diabetic mice. Here, novel near-infrared (NIR) named QX-B was designed synthesized to detect H2O2. For probe, quinolinium-xanthene dye used as fluorophore borate ester chosen response group. After addition H2O2, strong NIR fluorescence signal at 772 nm observed. The not only shows high sensitivity 10-fold enhancement but also displays excellent selectivity over other possible interfering species. In meantime, mechanism toward proposed verified high-performance liquid chromatography (HPLC) experiment, mass spectra (MS) density functional theory (DFT) calculation. Furthermore, based on low cell cytotoxicity QX-B, it been applied exogenous endogenous HeLa cells, HCT116 4T1 zebrafish successfully. More importantly, inspired performance fluorescence, monitoring mice first time. This provides very important information diagnosis treatment

Language: Английский

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Sensing and Bioimaging of the Gaseous Signaling Molecule Hydrogen Sulfide by Near-Infrared Fluorescent Probes

ACS Sensors, Journal Year: 2020, Volume and Issue: 5(11), P. 3365 - 3391

Published: Nov. 9, 2020

A fluorescent probe for the monitoring of H2S levels in living cells and organisms is highly desirable. In this regard, near-infrared (NIR) probes have emerged as a promising tool. NIR-I NIR-II many significant advantages; instance, NIR light penetrates deeper into tissue than at visible wavelengths, it causes less photodamage during biosample analysis autofluorescence, enabling higher signal-to-background ratios. Therefore, expected that having emission region are more suitable vivo imaging. Consequently, considerable increase reports new H2S-responsive appeared literature. This review highlights advances made developing aimed sensitive selective detection biological samples. Their applications real-time bioimaging cells/animals emphasized. The selection dyes designing probes, along with principles mechanisms involved sensing region, described. discussions focused on small-molecule nanomaterials-based probes.

Language: Английский

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Responsive small-molecule luminescence probes for sulfite/bisulfite detection in food samples

TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 136, P. 116199 - 116199

Published: Jan. 23, 2021

Language: Английский

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Near infrared fluorescent probes for detecting and imaging active small molecules

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 482, P. 215080 - 215080

Published: Feb. 21, 2023

Language: Английский

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Conditionally Activatable Photoredox Catalysis in Living Systems

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 144(1), P. 163 - 173

Published: Dec. 28, 2021

The transformational effect of photoredox catalytic chemistries has inspired new opportunities, enabling us to interrogate nature in ways that are not possible otherwise and unveil biotechnologies therapy diagnosis. However, the deployment artificial catalysis living systems remains challenging, mired by off-target risk safety concerns photocatalyst toxicity. Here, we present an appealing approach, namely conditionally activatable (ConAPC), as a proof concept design first ConAPC architecture (Se-NO2) based upon classic self-immolative chemistry, which inherent photocatalytic properties can be temporarily caged while species becomes active only at tumor sites via sensing specific biomarkers. Such masking strategy allows spatial–temporal control photoresponsivity vitro vivo. In particular, for design, biologically benign metal-free (Se-NH2), is able initiate NIR manipulate cellular electron pool O2-independent mechanism action, identified. With this unique strategy, potent tumor-specific targeting eradication (TGI: 95%) obtained mouse model. Impressively, favorable features such high-resolution recognition (SBR: 33.6) excellent biocompatibility also achieved. This work therefore offers possibility chemists leverage reactions toward development facile intelligent theranostics.

Language: Английский

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Excited-State Intramolecular Proton Transfer Dyes with Dual-State Emission Properties: Concept, Examples and Applications

Molecules, Journal Year: 2022, Volume and Issue: 27(8), P. 2443 - 2443

Published: April 10, 2022

Dual-state emissive (DSE) fluorophores are organic dyes displaying fluorescence emission both in dilute and concentrated solution the solid-state, as amorphous, single crystal, polycrystalline samples or thin films. This comes contrast to vast majority of fluorescent which typically show intense but quenched media solid-state owing π-stacking interactions; a well-known phenomenon called aggregation-caused quenching (ACQ). On contrary, molecular rotors with significant number free rotations have been engineered strong aggregated-state thanks restriction intramolecular motions. is concept aggregation-induced (AIE). DSE far less explored despite fact that they at crossroad ACQ AIE phenomena allow targeting applications (bio-conjugation, sensing, imaging) (organic electronics, data encryption, lasing, luminescent displays). Excited-State Intramolecular Proton Transfer (ESIPT) particularly suitable engineer dyes. Indeed, ESIPT fluorescence, relies on phototautomerism between normal tautomeric species, characterized by along large Stokes’ shift, an enhanced photostability sensitivity close environment, feature prone be used bio-sensing. A drawback needs overcome their weak intensity solution, detrimental motions excited-state. Several strategies proposed regard. In past few years, growing examples DSE-ESIPT indeed emerged literature, enriching database such attractive review aims brief concise overview exploitation luminescence for optimization properties. perspective, synergistic approach synthesis, spectroscopy ab initio calculations has proven efficient tool construction fluorophores.

Language: Английский

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