Spectroscopically dark phosphate features revealed by chemical exchange saturation transfer DOI Creative Commons
Jiaqi Lu, Joshua Straub, Mesopotamia S. Nowotarski

et al.

NMR in Biomedicine, Journal Year: 2023, Volume and Issue: 37(2)

Published: Oct. 18, 2023

Abstract Phosphate is an essential anion in the human body, comprising approximately 1% of total body weight, and playing a vital role metabolism, cell membranes, bone formation. We have recently provided spectroscopic, microscopic, computational evidence indicating that phosphates can aggregate much more readily solution than previously thought. This prior work indirect through observation unusual P NMR relaxation line‐broadening effects with increasing temperature. Here, we show that, under conditions slow exchange selective RF saturation, additional features become visible chemical saturation transfer (CEST) experiments, which appear to be related reported phosphate clustering. In particular, CEST shows pronounced dips several ppm upfield main resonance at low temperatures, while direct spectroscopy does not produce any signals range. study pH dependence these new spectroscopic present parameters based on fitting data. These findings could importance investigation dynamics, especially biological milieu.

Language: Английский

Single Cell Protein: A Potential Substitute in Human and Animal Nutrition DOI Open Access

Bogdan Constantin Bratosin,

Sorina Dârjan,

Dan Cristian Vodnar

et al.

Sustainability, Journal Year: 2021, Volume and Issue: 13(16), P. 9284 - 9284

Published: Aug. 18, 2021

Single cell protein (SCP) is the first product of fermentation process and has proven to be a good alternative. Food competition becoming more intense as world’s population continues grow. Soon, SCP may able compensate for deficit. Various global businesses are focusing on production, scope its application expanding time knowledge increases. High quantities can produced by microorganisms, such algae, yeast, fungi bacteria, due their fast development rate significant level in chemical structure. Beside proteins, contains carbohydrates, nucleic acids, lipids, minerals, vitamins several important amino acids. been an effective substitute expensive sources fish soybean products. In conclusion, easily replace traditional human animal feed without detrimental effects. Potential substrate candidates optimization strategies production have extensively studied. This review article focuses various aspects SCP, from using different substrates, player microorganisms nutritional benefits, economic aspects.

Language: Английский

Citations

136
ATP and Adenosine Metabolism in Cancer: Exploitation for Therapeutic Gain DOI Open Access
Gennady G. Yegutkin, Detlev Boison

Pharmacological Reviews, Journal Year: 2022, Volume and Issue: 74(3), P. 799 - 824

Published: June 23, 2022

Adenosine is an evolutionary ancient metabolic regulator linking energy state to physiologic processes, including immunomodulation and cell proliferation. Tumors create adenosine-rich immunosuppressive microenvironment through the increased release of ATP from dying stressed cells its ectoenzymatic conversion into adenosine. Therefore, adenosine pathway becomes important therapeutic target improve effectiveness immune therapies. Prior research has focused largely on two major ectonucleotidases, ectonucleoside triphosphate diphosphohydrolase 1/cluster differentiation (CD)39 ecto-5′-nucleotidase/CD73, which catalyze breakdown extracellular adenosine, subsequent activation different subtypes receptors with mixed findings antitumor protumor effects. New findings, needed for more effective approaches, require consideration redundant pathways controlling intratumoral levels, alternative NAD-inactivating CD38-ectonucleotide pyrophosphatase phosphodiesterase (ENPP)1-CD73 axis, counteracting ATP-regenerating pathway, cellular uptake phosphorylation by kinase. This review provides a holistic view intracellular metabolism as integrated complex network summarizes recent data underlying mechanisms precursors ADP control cancer immunosurveillance, tumor angiogenesis, lymphangiogenesis, cancer-associated thrombosis, blood flow, perfusion. Special attention given differences commonalities in purinome cancers, heterogeneity microenvironment, subcellular compartmentalization system, novel roles purine-converting enzymes targets therapy.

Significance Statement

The discovery role checkpoint led development strategies targeting signaling multiple clinical trials preclinical models. Here we identify gaps knowledge that need be filled gain agents key components and, this basis, provide network.

Language: Английский

Citations

92
Alkaline Phosphatase: A Reliable Endogenous Partner for Drug Delivery and Diagnostics DOI Creative Commons
Bao Le‐Vinh, Zeynep Burcu Akkuş‐Dağdeviren, Nguyet‐Minh Nguyen Le

et al.

Advanced Therapeutics, Journal Year: 2022, Volume and Issue: 5(2)

Published: Jan. 10, 2022

Abstract Since the 1960s membrane‐bound enzyme alkaline phosphatase (ALP) has been utilized in drug delivery. As it cleaves phosphate substructures from drugs, auxiliary agents, and even surface of nanocarriers, this enables design delivery systems that can alter their properties body on demand. Anionic nanocarriers exhibiting bioinert to interactive once having reached target site as due an ALP‐triggered cleavage anionic groups charge converts cationic improving for instance cellular uptake. Moreover, features such accumulation at or a targeted release triggered by ALP be introduced. In addition, is improve potential numerous diagnostic systems. Within review, one provides overview about activity, selectivity, distribution enzyme, well great variety applications diagnostics making use it.

Language: Английский

Citations

71
Multimetallic transition metal complexes:Luminescent probes for biomolecule sensing, ion detection, imaging and therapeutic application DOI
Bishnu Das, Parna Gupta

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 504, P. 215656 - 215656

Published: Jan. 19, 2024

Language: Английский

Citations

26
Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection DOI Creative Commons
Lucia Ruiz-Haddad, Muhammad Ali, Mario Pronk

et al.

Environmental Science and Ecotechnology, Journal Year: 2024, Volume and Issue: 21, P. 100387 - 100387

Published: Jan. 4, 2024

Currently, the most cost-effective and efficient method for phosphorus (P) removal from wastewater is enhanced biological P (EPBR) via polyphosphate-accumulating organisms (PAOs). This study integrates a literature review with genomic analysis to uncover phylogenetic metabolic diversity of relevant PAOs treatment. The findings highlight significant differences in capabilities Notably,

Language: Английский

Citations

19
History of ectonucleotidases and their role in purinergic signaling DOI
Herbert Zimmermann

Biochemical Pharmacology, Journal Year: 2020, Volume and Issue: 187, P. 114322 - 114322

Published: Nov. 6, 2020

Language: Английский

Citations

73
Targeting tumor microenvironment using tumor-infiltrating lymphocytes as therapeutics against tumorigenesis DOI
Hina Qayoom,

Shazia Sofi,

Manzoor Ahmad Mir

et al.

Immunologic Research, Journal Year: 2023, Volume and Issue: 71(4), P. 588 - 599

Published: April 1, 2023

Language: Английский

Citations

42
Discovering untapped microbial communities through metagenomics for microplastic remediation: recent advances, challenges, and way forward DOI Open Access
Atif Khurshid Wani, Nahid Akhtar,

Nafiaah Naqash

et al.

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(34), P. 81450 - 81473

Published: Jan. 13, 2023

Language: Английский

Citations

39
The cytoplasmic synthesis and coupled membrane translocation of eukaryotic polyphosphate by signal-activated VTC complex DOI Creative Commons
Zeyuan Guan,

Juan Chen,

Ruiwen Liu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Feb. 9, 2023

Inorganic polyphosphate (polyP) is an ancient energy metabolite and phosphate store that occurs ubiquitously in all organisms. The vacuolar transporter chaperone (VTC) complex integrates cytosolic polyP synthesis from ATP membrane translocation into the lumen. In yeast other eukaryotes, regulated by inositol pyrophosphate (PP-InsP) nutrient messengers, directly sensed VTC complex. Here, we report cryo-electron microscopy structure of signal-activated at 3.0 Å resolution. Baker's subunits Vtc1, Vtc3, Vtc4 assemble a 3:1:1 Fifteen trans-membrane helices form novel channel enabling transport newly synthesized PP-InsP binding orients catalytic polymerase domain entrance channel, both activating enzyme coupling translocation. Together with biochemical cellular studies, our work provides mechanistic insights biogenesis metabolite.

Language: Английский

Citations

35
Widespread occurrence and relevance of phosphate storage in foraminifera DOI Creative Commons
Nicolaas Glock, Julien Richirt, Christian Woehle

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Foraminifera are ubiquitous marine protists that intracellularly accumulate phosphate 1 , an important macronutrient in ecosystems and fertilizer potentially leaked into the ocean. Intracellular concentrations can be 100–1,000 times higher than surrounding water . Here we show storage foraminifera is widespread, from tidal flats to deep sea. The total amount of intracellular stored benthic foraminifer Ammonia confertitesta Wadden Sea during a bloom as high around 5% annual consumption phosphorus (P) Germany. Budget calculations for Southern North Peruvian Oxygen Minimum Zone indicate may buffer riverine P runoff approximately 37 days at about 21 margin. This indicates these organisms probably relevant cycling—they anthropogenic eutrophication coastal environments. Phosphate polyphosphate cell organelles acidocalcisomes. Their metabolic functions range regulation osmotic pressure pH calcium energy storage. In addition, energetic compounds, such creatine polyphosphate, adaptation O 2 depletion.

Language: Английский

Citations

1